A5009893675- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Advanced Fluorescence Microscopy Techniques
- Neural dynamics and brain function
- Lipid Membrane Structure and Behavior
- Neuroscience and Neural Engineering
- Cellular transport and secretion
- Neurogenesis and neuroplasticity mechanisms
- Retinal Development and Disorders
- Adenosine and Purinergic Signaling
- Photosynthetic Processes and Mechanisms
- Cell Image Analysis Techniques
- Medical Imaging and Pathology Studies
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Advanced Electron Microscopy Techniques and Applications
- Radiopharmaceutical Chemistry and Applications
- Advanced Memory and Neural Computing
- Electrochemical Analysis and Applications
- Memory and Neural Mechanisms
- Medical Imaging Techniques and Applications
Université de Bordeaux
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Institut Interdisciplinaire de Neuroscience
2015-2024
King's College London
2022-2024
Guy's Hospital
2023
ORCID
2020
The nanoscale organization of neurotransmitter receptors regarding pre-synaptic release sites is a fundamental determinant the synaptic transmission amplitude and reliability. How modifications in pre- post-synaptic machinery alignments affects currents, has only been addressed with computer modelling. Using single molecule super-resolution microscopy, we found strong spatial correlation between AMPA receptor (AMPAR) nanodomains adhesion protein neuroligin-1 (NLG1). Expression truncated form...
Despite the constant advances in fluorescence imaging techniques, monitoring endogenous proteins still constitutes a major challenge particular when considering dynamics studies or super-resolution imaging. We have recently evolved specific protein-based binders for PSD-95, main postsynaptic scaffold at excitatory synapses. Since synthetic recombinant recognize epitopes not directly involved target protein activity, we consider them here as tools to develop PSD-95 probes. After confirming...
Short-term plasticity of AMPAR currents during high-frequency stimulation depends not only on presynaptic transmitter release and postsynaptic recovery from desensitization, but also fast diffusion. How diffusion within the synapse regulates synaptic transmission millisecond scale remains mysterious. Using single-molecule tracking, we found that, upon glutamate binding, diffuse faster. stabilized in different conformational states by point mutations pharmacology, show that desensitized...
Long-term depression (LTD) of synaptic strength can take multiple forms and contribute to circuit remodeling, memory encoding or erasure. The generic term LTD encompasses various induction pathways, including activation NMDA, mGlu P2X receptors. However, the associated specific molecular mechanisms effects on physiology are still unclear. We here compare how NMDAR- P2XR-dependent affect nanoscale organization function in rodents. While both LTDs with a loss reorganization AMPARs, only...
Excitatory synapses are typically described as single synaptic boutons (SSBs), where one presynaptic bouton contacts a postsynaptic spine. Using serial section block-face scanning electron microscopy, we found that this textbook definition of the synapse does not fully apply to CA1 region hippocampus. Roughly half all excitatory in stratum oriens involved multi-synaptic (MSBs), containing multiple active zones contacted many spines (from 2 7) on basal dendrites different cells. The fraction...
Abstract Plasticity at excitatory synapses can be induced either by synaptic release of glutamate or the gliotransmitters such as ATP. Recently, we showed that postsynaptic P2X2 receptors activated ATP released from astrocytes downregulate AMPAR, providing a novel mechanism which glial cells modulate activity. ATP- and lNMDA-induced depression in CA1 region hippocampus are additive, suggesting distinct molecular pathways. AMPARs homo-or hetero-tetramers composed GluA1-A4. Here, first show...
ABSTRACT Despite the constant advances in fluorescence imaging techniques, monitoring endogenous proteins still constitutes a major challenge particular when considering dynamics studies or super-resolution imaging. We have recently evolved specific protein-based binders for PSD-95, main postsynaptic scaffold at excitatory synapses. Since synthetic recognize epitopes not directly involved target protein activity, we consider them here as tools to develop PSD-95 probes. After confirming their...
Summary The nanoscale organization of neurotransmitter receptors relative to pre-synaptic release sites is a fundamental determinant both the amplitude and reliability synaptic transmission. How modifications in alignment between pre- post-synaptic machineries affect current properties has only been addressed with computer modeling, therefore remains hypothetical. Using dual-color single molecule based super-resolution microscopy, we found strong spatial correlation AMPA receptor (AMPAR)...
Information transfer at synapses occurs when vesicles fuse with the plasma membrane to release neurotransmitters, which then bind receptors postsynaptic membrane. The process of neurotransmitter varies dramatically between different synapses, but little is known about how this heterogeneity emerges. development super-resolution microscopy has revealed that synaptic proteins are precisely organised within and two parts synapse precise spatiotemporal organisation fine-tunes neurotransmission....
Chemical synapses control their strength through the nanoscale clustering of postsynaptic receptors into sub-synaptic domains (SSDs). Despite importance in synapse function, properties and plasticity these are not well understood vivo, particularly inhibitory synapses. We used direct Stochastic Optical Resolution Microscopy (dSTORM) to show that Gephyrin, main receptor scaffold protein, is organised SSDs with distinct arrangements depending on sub-cellular location presynaptic partner....
Abstract Excitatory synapses are typically described as single synaptic boutons (SSBs), where one presynaptic bouton contacts a postsynaptic spine. Using serial section block face scanning electron microscopy, we found that this textbook definition of the synapse does not fully apply to CA1 region hippocampus. Roughly half all excitatory in stratum oriens involved multi-synaptic (MSBs), containing multiple active zones contacted many spines (from 2 7) on basal dendrites different cells. The...
Long-Term Potentiation (LTP) and Depression (LTD) of excitatory synaptic transmission are considered as cellular basis learning memory. These two forms plasticity have been mainly attributed to global changes in the number AMPA-type glutamate receptor (AMPAR) through a regulation diffusion/trapping balance at PSD, exocytosis endocytosis. While precise molecular mechanisms base LTP intensively investigated, ones involved LTD remains elusive. Here we combined super-resolution imaging...