A5077536869- Autophagy in Disease and Therapy
- Neurogenesis and neuroplasticity mechanisms
- Cannabis and Cannabinoid Research
- Neuroscience and Neuropharmacology Research
- Nerve injury and regeneration
- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Pluripotent Stem Cells Research
- Parkinson's Disease Mechanisms and Treatments
- Sleep and Wakefulness Research
- Spaceflight effects on biology
- Heat shock proteins research
- MicroRNA in disease regulation
- Cell death mechanisms and regulation
- Axon Guidance and Neuronal Signaling
- Nanoparticles: synthesis and applications
- Molecular Biology Techniques and Applications
- Muscle Physiology and Disorders
- Phagocytosis and Immune Regulation
- Thermoregulation and physiological responses
- Genetics and Physical Performance
- Epigenetics and DNA Methylation
University of Lausanne
2020-2025
Foundation for Research and Technology Hellas
2011-2020
FORTH Institute of Molecular Biology and Biotechnology
2013-2016
University of Crete
2012
University of Basel
2007-2011
Topoisomerases are essential for DNA replication in dividing cells, but their genomic targets and function postmitotic cells remain poorly understood. Here we show that a switch the expression from IIα (Top2α) to IIβ (Top2β) occurs during neuronal differentiation vitro vivo. Genome-scale location analysis stem cell–derived neurons reveals Top2β binding chromosomal sites methylated at lysine 4 of histone H3, feature regulatory regions. Indeed Top2β-bound preferentially promoters become...
Long-term depression (LTD) of synaptic strength can take multiple forms and contribute to circuit remodeling, memory encoding or erasure. The generic term LTD encompasses various induction pathways, including activation NMDA, mGlu P2X receptors. However, the associated specific molecular mechanisms effects on physiology are still unclear. We here compare how NMDAR- P2XR-dependent affect nanoscale organization function in rodents. While both LTDs with a loss reorganization AMPARs, only...
The pruning of dendritic spines during development requires autophagy. This process is facilitated by long-term depression (LTD)-like mechanisms, which has led to speculation that LTD, a fundamental form synaptic plasticity, also Here, we show the induction LTD via activation NMDA receptors or metabotropic glutamate initiates autophagy in postsynaptic dendrites mice. Dendritic autophagic vesicles (AVs) act parallel with endocytic machinery remove AMPA receptor subunits from membrane for...
The nervous system becomes increasingly vulnerable to insults and prone dysfunction during ageing. Age-related decline of neuronal function is manifested by the late onset many neurodegenerative disorders, as well reduced signalling processing capacity individual neuron populations. Recent findings indicate that impairment Ca2+ homeostasis underlies increased susceptibility neurons damage, associated with ageing process. However, impact on in remains largely unknown. Here, we survey...
Abstract Autophagy provides nutrients during starvation and eliminates detrimental cellular components. However, accumulating evidence indicates that autophagy is not merely a housekeeping process. Here, by combining mouse models of neuron‐specific ATG5 deficiency in either excitatory or inhibitory neurons with quantitative proteomics, high‐content microscopy, live‐imaging approaches, we show protein functions to regulate cAMP‐dependent kinase A (PKA)‐mediated phosphorylation...
Autophagy disorders prominently affect the brain, entailing neurodevelopmental and neurodegenerative phenotypes in adolescence or aging, respectively. Synaptic behavioral deficits are largely recapitulated mouse models with ablation of autophagy genes brain cells. Yet, nature temporal dynamics autophagic substrates remain insufficiently characterized. Here, we immunopurified LC3-positive vesicles (LC3-pAVs) from proteomically profiled their content. Moreover, characterized LC3-pAV content...
Here we show a novel function for Retinoblastoma family member, p107 in controlling stem cell expansion the mammalian brain. Adult p107-null mice had elevated numbers of proliferating progenitor cells their lateral ventricles. In vitro neurosphere assays revealed striking increases number forming from p107−/− brains that exhibited enhanced capacity self-renewal. An expanded population p107-deficient was shown vivo by (a) increased slowly cycling ventricles; and (b) accelerated rates neural...
ABSTRACT The aging central nervous system (CNS) is often marked by myelin degeneration, yet the underlying mechanisms remain elusive. This study delves into previously unexplored role of autophagy in maintaining CNS during aging. We generated transgenic mouse line plpCre ERT2 ; atg5 f/f , enabling selective deletion core autophagic component Atg5 oligodendrocytes (OLs) following tamoxifen administration adulthood, while analysis was conducted on aged mice. Our findings reveal that...
(Macro)autophagy is a major lysosome-dependent degradation mechanism which engulfs, removes and recycles unwanted cytoplasmic material, including damaged organelles toxic protein aggregates. Although few studies implicate autophagy in CNS demyelinating pathologies, its role, particularly mature oligodendrocytes myelin, remains poorly studied. Here, using both pharmacological genetic inhibition of the autophagic machinery, we provide evidence that an essential for oligodendrocyte maturation...