A5101969787- Advanced Fluorescence Microscopy Techniques
- Salivary Gland Tumors Diagnosis and Treatment
- Photoreceptor and optogenetics research
- Cancer Diagnosis and Treatment
- Near-Field Optical Microscopy
- Head and Neck Cancer Studies
- Advanced Electron Microscopy Techniques and Applications
- TiO2 Photocatalysis and Solar Cells
- Head and Neck Surgical Oncology
- Neuroscience and Neuropharmacology Research
- Cell Image Analysis Techniques
- Neural dynamics and brain function
- Plant Molecular Biology Research
- Chemical Synthesis and Analysis
- Collagen: Extraction and Characterization
- Ear and Head Tumors
- Tracheal and airway disorders
- Diagnosis and Treatment of Venous Diseases
- Vascular anomalies and interventions
- Photochemistry and Electron Transfer Studies
- Orbital Angular Momentum in Optics
- Sperm and Testicular Function
- Bone Tumor Diagnosis and Treatments
- Spectroscopy and Quantum Chemical Studies
- Advanced Fiber Optic Sensors
Sainsbury Laboratory
2022-2024
University of Cambridge
2018-2024
Bridge University
2024
Université de Bordeaux
2019
University of Pittsburgh Medical Center
2019
Institut Interdisciplinaire de Neuroscience
2019
Imperial College London
2011-2013
Goethe University Frankfurt
2005-2010
Bundeswehrkrankenhaus
2009
Jena University Hospital
2007
Abstract The plant hormone abscisic acid (ABA) accumulates under abiotic stress to recast water relations and development. To overcome a lack of high-resolution sensitive reporters, we developed ABACUS2s—next-generation Förster resonance energy transfer (FRET) biosensors for ABA with high affinity, signal-to-noise ratio orthogonality—that reveal endogenous patterns in Arabidopsis thaliana . We mapped stress-induced dynamics resolution the cellular basis local systemic functions. At reduced...
During gastrulation, embryonic cells become specified into distinct germ layers. In mouse, this continues throughout somitogenesis from a population of bipotent stem called neuromesodermal progenitors (NMps). However, the degree self-renewal associated with NMps in fast-developing zebrafish embryo is unclear. Using genetic clone-tracing method, we labelled early and found strong clonal similarity between spinal cord mesoderm tissues. We followed individual cell lineages using light-sheet...
At the flick of a switch: Two side chains collagen peptide containing (2S,4S)-mercaptoproline at two defined positions are linked with diiodo azobenzene derivative. With trans isomer clamp (orange), folds into triple helix (green, blue, gray), which unfolds upon irradiation 330 nm. The light-controlled folding/unfolding processes fully reversible, making this system well-suited for ultrafast spectroscopic analysis. Supporting information article is available on WWW under...
We introduce single molecule light field microscopy (SMLFM), a new class of three-dimensional (3D) localization microscopy. By segmenting the back focal plane microscope objective with an array microlenses to generate multiple 2D perspective views, same fluorophore can be imaged from different angles. These in combination bespoke fitting algorithm, enable 3D positions fluorophores determined parallax. SMLFM achieves up 20 nm precision throughout extended <mml:math...
The early steps in the photocycle of bacterial proton pump proteorhodopsin (PR) were analyzed by ultrafast pump/probe spectroscopy to compare rate retinal isomerization at alkaline and acidic pH values. At 9, functionally important primary acceptor (Asp97, pK(a) = 7.7) is negatively charged; consequently, a reaction cycle analogous archaeal bacteriorhodopsin (BR) observed. excited electronic state PR displays pronounced biphasic decay with time constants 400 fs 8 ps. 6 where Asp97 protonated...
Summary— Between May 1981 and October 1982, 259 varicoceles were identified by phlebography. It was possible to occlude 217 with Varicocid™ using a percutaneous Seldinger catheterisation technique under local anaesthesia. This method is an alternative standard surgical techniques has several advantages. cheap, it can be carried out on outpatient basis, lacks the risks associated general anaesthesia there are few complications.
The results of 230 retrograde phlebographies the V. spermatica int., 165 percutaneous sclerotherapies and 60 follow-up on subfertile patients are presented. In 24 (10%) complications occurred, yet in none cases clinical damage became evident. Percutaneous sclerotherapie is contraindicated, when selective probing int. impossible or massive collaterals to other venous systems could lead an uncontrolled spread sclerosing agent. Treatment idiopathic varicocele men by sclerotherapy a safe...
The dependence of the interfacial electron transfer in alizarin-sensitized TiO2 nanoparticles on sample pH has been examined via transient absorbance spectroscopy visible spectral region (443-763 nm). Excitation alizarin/TiO2 system with pump pulses (lambdaexc = 500 nm) leads to a very fast injection (tauinj < 100 fs) over wide range. Back shows complicated multiphasic kinetics and strongly depends acidity solution. strong back-electron-transfer dynamics ambient value is explained by...
A critical review of the experience with extracorporeal shockwave lithotripsy (ESWL) gallbladder stones is needed to clarify whether this method should continue be applied patients.Patients symptomatic were treated by piezoelectric ESWL according a prospective protocol between 1988 and 1997. treatment was limited maximum three (solitary <20 mm diameter) five sessions (larger solitary or multiple stones) 3000 pulses per session. Univariate multivariate analyses pretreatment variables...
Strukturen aufgedröselt: Die beiden Seitenketten eines Collagenpeptids mit (2S,4S)-Mercaptoprolin an zwei definierten Positionen wurden durch ein Diiodazobenzol als Verbindungsstück verknüpft. Das Peptid faltet der trans-konfigurierten Azobenzolklammer (orange) in eine Tripelhelix (grün, blau und grau) entfaltet bei Belichtung 330 nm. lichtgesteuerten Faltungs- Entfaltungsvorgänge sind völlig reversibel, sodass dieses System für ultraschnelle Spektroskopie prädestiniert ist.
The first steps of the photocycle D97N mutant proteorhodopsin (PR) have been investigated by means ultrafast transient absorption spectroscopy. A comparison with primary dynamics native PR and D85N bacteriorhodopsin is given. Upon photoexcitation covalently bound all-trans retinal excited state decays biexponentially time constants 1.4 20 ps via a conical intersection, resulting in 13-cis isomerized retinal. Neither two-deactivation channels significantly preferred. slowed down at pH 9...
ABSTRACT The shoot apical meristem (SAM), located at the plant apex, is accountable for formation of above-ground organs such as leaves, stem and flowers. Although transcriptional profiling has elucidated some cell-types observed within stems or flowers, differentiation dynamics from cells to multiple cell identities remain unknown. We employed a single-nucleus RNA-sequencing approach assess heterogeneity processes SAM. By collecting dissected inflorescence meristems, we constructed an SAM...
We present a stimulated emission depletion (STED) fluorescence lifetime imaging (FLIM) microscope, excited by microstructured optical fibre supercontinuum source that is pumped femtosecond Ti:Sapphire-laser, which also used for depletion. Implemented using piezo-scanning stage on laser scanning confocal microscope system with FLIM realised time correlated single photon counting (TCSPC), this provides convenient switching between and STED-FLIM spatial resolution down to below 60 nm. will our...
Specific control of photoinduced molecular reactions is desired for various reasons, not least to increase photostability by avoiding harmful, reactive intermediate states. In this paper we will present a way specifically deactivate the excited-state merocyanines selectively addressing additional pathways. The photodynamics different in solution as well coupled semiconductor nanoparticles have primarily been investigated transient absorption spectroscopy. For free dye, two main fast...
The early steps in the photocycle of sensory rhodopsin II mutant D75N are investigated a comprehensive study using femtosecond visible pump/probe spectroscopy. An overall slower response dynamics after photoexcitation is observed compared to wild-type II, which assigned changed electrostatics and an altered hydrogen-bonding network within retinal binding pocket. Furthermore, influence azide on primary reaction analyzed. addition accelerates sub-10 ps nearly rates found wild-type. Moreover,...
Super-resolution techniques that localize single molecules in three dimensions through point spread function (PSF) engineering are very sensitive to aberrations and optical alignment. Here we show how double-helix is affected by such mis-alignment aberration. Specifically, demonstrate simulation experiment misplacement of phase masks infinity corrected systems a common source significant loss accuracy. We also describe an optimal alignment calibration procedure correct for these errors. In...