A5044517069- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Endoplasmic Reticulum Stress and Disease
- Genetic Neurodegenerative Diseases
- Alzheimer's disease research and treatments
- Advanced NMR Techniques and Applications
- Ubiquitin and proteasome pathways
- Nicotinic Acetylcholine Receptors Study
- Neural dynamics and brain function
- Protein Structure and Dynamics
- RNA Research and Splicing
- Molecular Communication and Nanonetworks
- Neurological diseases and metabolism
- Prion Diseases and Protein Misfolding
Max Planck Institute of Biochemistry
2011-2019
Goethe University Frankfurt
2006-2011
Amyloid-like protein aggregation is associated with neurodegeneration and other pathologies. The nature of the toxic aggregate species their mechanism action remain elusive. Here, we analyzed compartment specificity toxicity using artificial β-sheet proteins, as well fragments mutant huntingtin TAR DNA binding protein-43 (TDP-43). Aggregation in cytoplasm interfered nucleocytoplasmic RNA transport. In contrast, same proteins did not inhibit transport when forming inclusions nucleus at or...
The proteorhodopsin (PR) family found in bacteria near the ocean's surface consists of hundreds PR variants color-tuned to their environment. contains a highly conserved single histidine at position 75, which is not most other retinal proteins. Using 13C and 15N MAS NMR, we were able prove for green that His75 forms pH-dependent H-bond with primary proton acceptor Asp97, explains its unusually high pKa. functional role has been studied using site-directed mutagenesis time-resolved optical...
The first steps of the photocycle D97N mutant proteorhodopsin (PR) have been investigated by means ultrafast transient absorption spectroscopy. A comparison with primary dynamics native PR and D85N bacteriorhodopsin is given. Upon photoexcitation covalently bound all-trans retinal excited state decays biexponentially time constants 1.4 20 ps via a conical intersection, resulting in 13-cis isomerized retinal. Neither two-deactivation channels significantly preferred. slowed down at pH 9...