A5045442881- Prostate Cancer Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- Urologic and reproductive health conditions
- Urinary Bladder and Prostate Research
- Radiomics and Machine Learning in Medical Imaging
- Cancer Cells and Metastasis
- Cardiac, Anesthesia and Surgical Outcomes
- Surgical Simulation and Training
- Bladder and Urothelial Cancer Treatments
- Colorectal Cancer Surgical Treatments
- Urological Disorders and Treatments
- Advanced Radiotherapy Techniques
- Hormonal and reproductive studies
- MRI in cancer diagnosis
- Peripheral Nerve Disorders
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- PARP inhibition in cancer therapy
- Pelvic floor disorders treatments
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Sexual function and dysfunction studies
- Genital Health and Disease
- Kidney Stones and Urolithiasis Treatments
University College London
2016-2025
University College London Hospitals NHS Foundation Trust
2007-2025
Royal London Hospital
2009-2025
University College Hospital
2015-2024
University College Hospital at Westmoreland Street
2016-2024
National Health Service
2019-2023
University of Virginia
2000-2023
Queen Mary University of London
2007-2023
Barts Health NHS Trust
2017-2023
The London College
2021
Abstract Emerging data demonstrate homologous recombination (HR) defects in castration-resistant prostate cancers, rendering these tumours sensitive to PARP inhibition. Here we a direct requirement for the androgen receptor (AR) maintain HR gene expression and activity cancer. We show that PARP-mediated repair pathways are upregulated cancer following androgen-deprivation therapy (ADT). Furthermore, upregulation of is essential survival cells synthetic lethality between ADT inhibition vivo....
Castration-resistant prostate cancer (CRPC) is the major cause of death from cancer. Biomarkers to improve early detection and prediction CRPC especially using non-invasive liquid biopsies could outcomes. Therefore, we investigated plasma exosomal miRNAs associated with their potential for development into biomarkers resistance treatment. RNA-sequencing, which generated approximately five million reads per patient, was performed identify differentially expressed in 24 treatment-naive...
The androgen receptor (AR) is the dominant growth factor in prostate cancer (PCa). Therefore, understanding how ARs regulate human transcriptome of paramount importance. early effects castration on PCa have not previously been studied 27 patients medically castrated with degarelix 7 d before radical prostatectomy. We used mass spectrometry, immunohistochemistry, and gene expression array (validated by reverse transcription-polymerase chain reaction) to compare resected tumour matched,...
The Prostate Imaging Quality (PI-QUAL) score assesses the quality of multiparametric MRI (mpMRI). A 1 means all sequences are below minimum standard diagnostic quality, 3 implies that scan is sufficient and 5 three optimal quality. We investigated inter-reader reproducibility PI-QUAL in patients enrolled NeuroSAFE PROOF trial.We analysed scans 103 on different MR systems vendors from 12 hospitals. Two dedicated radiologists highly experienced prostate mpMRI independently assessed for each...
Aberrant DNA methylation plays a pivotal role in carcinogenesis and its mapping is likely to provide biomarkers for improved diagnostic risk assessment prostate cancer (PCa). We quantified compared absolute levels among 28 candidate genes 48 PCa 29 benign hyperplasia (BPH) samples using the pyrosequencing (PSQ) method identify with prognostic potential. RARB, HIN1, BCL2, GSTP1, CCND2, EGFR5, APC, RASSF1A, MDR1, NKX2-5, CDH13, DPYS, PTGS2, EDNRB, MAL, PDLIM4, HLAa, ESR1 TIG1 were highly...
Aberrant DNA methylation plays a pivotal role in carcinogenesis and its mapping is likely to provide biomarkers for improved diagnostic risk assessment prostate cancer (PCa). We quantified compared absolute levels among 28 candidate genes 48 PCa 29 benign hyperplasia (BPH) samples using the pyrosequencing (PSQ) method identify with prognostic potential. RARB, HIN1, BCL2, GSTP1, CCND2, EGFR5, APC, RASSF1A, MDR1, NKX2-5, CDH13, DPYS, PTGS2, EDNRB, MAL, PDLIM4, HLAa, ESR1 TIG1 were highly...
Identification of men harbouring insignificant prostate cancer (PC) is important in selecting patients for active surveillance. Tools have been developed PSA-screened populations to identify such based on clinical and biopsy parameters.Prospectively collected case series 848 was treated with radical prostatectomy between July 2007 October 2011 at an English tertiary care centre. Tumour volume assessed by pathological examination. For each tool, receiver operator characteristics were...
Abstract Many human cancers present as multifocal lesions. Understanding the clonal origin of is both etiological and clinical importance. The molecular basis prostate cancer has previously been explored using a limited number isolated markers and, although independent widely believed, still debatable. We attempted to address genome‐wide copy‐number analysis individual high‐grade prostatic intraepithelial neoplasia (HGPIN) Using Affymetrix array 6.0 analysis, we compared genomic changes...
Purpose: To develop an approach for the investigation of different subtypes circulating tumor cells (CTC) and other to evaluate their potential prognostic value prostate cancer.Experimental Design: Malignancy CTCs undergoing epithelial-to-mesenchymal transition (EMT) was confirmed by repeated FISH. Subgroups in 81 patients with cancer (43 castration resistant 38 untreated localized) were correlated disease aggressiveness parameters. AUC analysis applied compare performance metastasis...
The androgen receptor (AR) is a major drug target in prostate cancer (PCa). We profiled the AR-regulated kinome to identify clinically relevant and druggable effectors of AR signaling. Using genome-wide approaches, we interrogated all regulated kinases. Among these, choline kinase alpha (CHKA) expression was evaluated benign (n = 195), prostatic intraepithelial neoplasia (PIN) 153) (PCa) lesions 359). how CHKA regulates signaling using biochemical assays investigated regulation men with PCa,...
Background In men suspected of having prostate cancer (PCa), up to 50% with positive multiparametric MRI (mpMRI) findings (Prostate Imaging Reporting and Data System [PI-RADS] or Likert score 3 higher) have no clinically significant (Gleason ≤3+3, benign) biopsy findings. Vascular, Extracellular, Restricted Diffusion for Cytometry in Tumor (VERDICT) analysis could improve the stratification mpMRI Purpose To evaluate VERDICT MRI, mpMRI-derived apparent diffusion coefficient (ADC),...
Background: The accuracy of multi-parametric MRI (mpMRI) in the pre-operative staging prostate cancer (PCa) remains controversial. Objective: purpose this study was to evaluate ability mpMRI accurately predict PCa extra-prostatic extension (EPE) on a side-specific basis using risk-stratified 5-point Likert scale. This also aimed assess influence scan quality diagnostic accuracy. Patients and Methods: We included 124 men who underwent robot-assisted RP (RARP) as part NeuroSAFE PROOF at our...
The COVID-19 pandemic has posed daunting challenges when conducting clinical research. Adopting new technologies such as remote electronic consent (e-Consent) can help overcome them. However, guidelines for e-Consent implementation in ongoing trials are currently lacking. NeuroSAFE PROOF trial is a randomized evaluating the role of frozen section analysis during RARP prostate cancer. In response to crisis, recruitment was halted, and solution designed. aim this paper describe process...
Background Docetaxel improves overall survival (OS) in castration-resistant prostate cancer (PCa) (CRPC) and metastatic hormone-sensitive PCa (mHSPC). However, not all patients respond due to inherent and/or acquired resistance. There remains an unmet clinical need for a robust predictive test stratify treatment. Liquid biopsy of circulating tumour cell (CTCs) is minimally invasive, can provide real-time information the heterogeneous therefore may be potentially ideal docetaxel response...
Overdiagnosis is arguably the greatest challenge in management of men with prostate cancer. Multi-parametric (mp)-MRI prior to biopsy may have a role ruling-in and ruling-out clinically significant disease.114 consecutive at risk cancer previous underwent mp-MRI transrectal ultrasound (TRUS)-guided biopsies. Standard systematic biopsies were carried out targeting suspicious areas.59.6% had detected by TRUS-guided biopsy. Mean age was 63.6 years (SD 9.0). If not been biopsied because negative...
To propose and evaluate a technique for automatic quantification of fissural completeness from chest computed tomography (CT) in database subjects with severe emphysema.Ninety-six CT studies patients emphysema were included. The lungs, fissures lobes automatically segmented. the was calculated as percentage lobar border defined by fissure. score method compared visual consensus read three radiologists using boxplots, rank sum tests ROC analysis.The found 49% (47/96), 15% (14/96) 67% (64/96)...
Early detection and treatment of asymptomatic men with advanced high-risk prostate cancer (PCa) may improve survival rates. To determine outcomes for diagnosed PCa following prostate-specific antigen (PSA) testing who were excluded from the ProtecT randomised trial. Mortality was compared 492 followed up a median 7.4 yr to contemporaneous cohort UK Anglia Cancer Network (ACN) matched subset ACN. PCa-specific all-cause mortality using Kaplan-Meier analysis Cox's proportional hazards...
Objectives To report on the methods, peri‐operative outcomes and histopathological concordance between frozen final section from NeuroSAFE PROOF feasibility study (NCT03317990). Patients Methods Between May 2018 March 2019, 49 patients at two UK centres underwent robot‐assisted radical prostatectomy (RARP). Twenty‐five patient were randomized to RARP (intervention arm) 24 standard (control arm). Frozen was compared paraffin margin assessment in 25 arm. Operation timings complications...
Sparing the periprostatic neurovascular bundles during robot-assisted radical prostatectomy (RARP) improves postoperative erectile function and early urinary continence recovery. The NeuroSAFE technique, a standardised frozen section analysis, enables accurate real-time detection of positive surgical margins nerve-sparing, increasing likelihood successful nerve preservation. However, impact technique on patient outcomes remains uncertain. We aimed to assess effect NeuroSAFE-guided RARP...