A5045657404- Alzheimer's disease research and treatments
- Parkinson's Disease Mechanisms and Treatments
- Neurological disorders and treatments
- Neurological diseases and metabolism
- Mitochondrial Function and Pathology
- Cell death mechanisms and regulation
- ATP Synthase and ATPases Research
- Cancer therapeutics and mechanisms
- RNA Interference and Gene Delivery
- Synthesis and biological activity
- Neuroinflammation and Neurodegeneration Mechanisms
- Autophagy in Disease and Therapy
- Prion Diseases and Protein Misfolding
- Cholinesterase and Neurodegenerative Diseases
- Botulinum Toxin and Related Neurological Disorders
- Photoreceptor and optogenetics research
- Advanced biosensing and bioanalysis techniques
- Spanish History and Politics
- Bioactive Compounds and Antitumor Agents
- Extracellular vesicles in disease
- RNA and protein synthesis mechanisms
- Nerve injury and regeneration
- Ginkgo biloba and Cashew Applications
- Philosophy, Ethics, and Existentialism
- Quinazolinone synthesis and applications
Institut des Maladies Neurodégénératives
2017-2024
Centre National de la Recherche Scientifique
2017-2024
Université de Bordeaux
2008-2024
Inserm
2007-2021
Université de Poitiers
2017-2021
Laboratoire de Neurosciences Expérimentales et Cliniques
2017-2020
Institut Bergonié
2005-2012
Bordeaux Population Health
2011
Centre Hospitalier Universitaire de Bordeaux
2009
University of Padua
1999-2009
The dentate gyrus of the hippocampus is one few regions mammalian brain where new neurons are generated throughout adulthood. This adult neurogenesis has been proposed as a novel mechanism that mediates spatial memory. However, data showing causal relationship between and memory controversial. Here, we developed an inducible transgenic strategy allowing specific ablation adult-born hippocampal neurons. resulted in impairment relational memory, which supports capacity for flexible,...
Mitochondria are key players of apoptosis and can irreversibly commit the cell to death by releasing cytochrome c (Cyt.c) cytosol, where caspases 9 3 subsequently get activated. Under conditions oxidative stress, opening mitochondrial permeability transition pore (PTP) represents an early trigger is crucial in causing Cyt.c release. To account for latter, current models propose that PTP gating would result, as case vitro, rupture outer membrane caused matrix swelling. Using live imaging...
Abstract Transportation of key proteins via extracellular vesicles has been recently implicated in various neurodegenerative disorders, including Parkinson’s disease, as a new mechanism disease spreading and source biomarkers. Extracellular likely to be derived from the brain can isolated peripheral blood have reported contain higher levels α-synuclein (α-syn) patients. However, very little is known about multiple system atrophy, that, like involves pathological α-syn aggregation, though...
Stealth α-synuclein amyloids proliferate in preparations of preformed fibrils and form aggressive self-replicating strains.
Aggregated alpha-synuclein (α-syn) is a principal constituent of Lewy bodies (LBs) and glial cytoplasmic inclusions (GCIs) observed respectively inside neurons in Parkinson's disease (PD) oligodendrocytes multiple system atrophy (MSA). Yet, the cellular origin, pathophysiological role, mechanism formation these (IBs) remain to be elucidated. It has recently been proposed that α-syn IBs eventually cause demise host cell by virtue cumulative sequestration partner proteins organelles. In...
α-Synucleinopathies are spreading neurodegenerative disorders characterized by the intracellular accumulation of insoluble aggregates populated α-Synuclein (α-Syn) fibrils. In Parkinson’s disease (PD) and dementia with Lewy bodies, intraneuronal α-Syn referred to as bodies in somata neurites neuronal processes. multiple system atrophy (MSA) also found within mature oligodendrocytes (OLs) where they form Glial Cytoplasmic Inclusions (GCIs). However, origin GCIs remains enigmatic: (i) OLs do...
The antioxidant properties of α-tocopherol have been proposed to play a beneficial chemopreventive role against cancer. However, emerging data also indicate that it may exert contrasting effects on the efficacy chemotherapeutic treatments when given as dietary supplement, being in case harmful for patients. This dual and, particular, its anticancer drugs remains poorly documented. For this purpose, we studied here, using high throughput flow cytometry, direct impact apoptosis and cell cycle...
Polyphenolic ellagitannins are natural compounds that often associated with the therapeutic activity of plant extracts used in traditional medicine. They display cancer-preventing animal models by a mechanism remains unclear. Potential targets have been proposed, including DNA topoisomerases II (Top2). Top2α and Top2β, two isoforms human Top2, play crucial role regulation replication, transcription, chromosome segregation. target anticancer agents clinic such as anthracyclines (e.g.,...
Fas triggers apoptosis via the caspase cascade when bound to its ligand FasL. In type I cells, is concentrated into plasma membrane lipid rafts, and these domains are required for apoptotic signal occur. contrast, excluded from microdomains in II cells. We report that coligation with of receptor CD28 strongly increases Fas-induced T lymphocytes, whereas it has no effect a cell line. The independent intracellular region requires recruitment microdomains. Indeed, upon costimulation,...
The synucleinopathy underlying multiple system atrophy (MSA) is characterized by the presence of abundant amyloid inclusions containing fibrillar α-synuclein (α-syn) aggregates in brains patients and associated with an extensive neurodegeneration. In contrast to Parkinson’s disease (PD) where pathological α-syn are almost exclusively neuronal, MSA principally observed oligodendrocytes (OLs) they form glial cytoplasmic (GCIs). This intriguing because differentiated OLs express low levels...
The distinct neuropathological features of the different α-Synucleinopathies, as well diversity α-Synuclein (α-Syn) intracellular inclusion bodies observed in post mortem brain sections, are thought to reflect strain characterizing invasive α-Syn amyloids. However, this "one strain, one disease" view is still hypothetical, and date, a possible disease-specific contribution non-amyloid factors has not been ruled out. In Multiple System Atrophy (MSA), buildup inclusions oligodendrocytes seems...
Abstract The neuropathology of α-Synucleinopathies (αSP) is characterized by the spread subcellular inclusions containing fibrils made stacked-up α-Synuclein (α-Syn) monomers. repetitive amyloid fold adopted α-Syn has now been at atomic scale. However, direct observation using routine immuno-histological procedures remains an issue. In particular, widely used phosphorylated (pS129) only a surrogate marker aggregation. We report here that pS129 misleading in overexpression-based models which...
The progressive accumulation of misfolded α-synuclein (α-syn) in the brain is widely considered to be causal for debilitating clinical manifestations synucleinopathies including, most notably, Parkinson's disease (PD). Immunotherapies, both active and passive, against α-syn have been developed are promising novel treatment strategies such disorders. To increase potency specificity PD vaccination, we created 'Win Skin Immune System Trick' (WISIT) vaccine platform designed target skin-resident...
Recombinant fluorescent probes allow the detection of molecular events inside living cells. Many them exploit intracellular space to provide positional signals and, thus, require by single cell imaging. We describe here a novel strategy based on capable encoding spatial dimension into "all-or-none" fluorescence intensity changes (differential anchorage probes, DAPs). The resulting can be acquired in cells at high throughput automated flow cytometry, (i) bypassing image acquisition and...