A5075473661- Advanced NMR Techniques and Applications
- Prion Diseases and Protein Misfolding
- Alzheimer's disease research and treatments
- Lipid Membrane Structure and Behavior
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Legume Nitrogen Fixing Symbiosis
- Protein Structure and Dynamics
- Advanced MRI Techniques and Applications
- Solid-state spectroscopy and crystallography
- Muon and positron interactions and applications
- Photosynthetic Processes and Mechanisms
- Supramolecular Self-Assembly in Materials
- Trace Elements in Health
- Escherichia coli research studies
- NMR spectroscopy and applications
- Bacterial biofilms and quorum sensing
- Parkinson's Disease Mechanisms and Treatments
- Amyotrophic Lateral Sclerosis Research
- Caveolin-1 and cellular processes
- Neurological diseases and metabolism
- Plant Reproductive Biology
- RNA and protein synthesis mechanisms
- Synthesis and Properties of Aromatic Compounds
Centre National de la Recherche Scientifique
2016-2025
Institut Européen de Chimie et Biologie
2015-2025
Université de Bordeaux
2016-2025
Institut Polytechnique de Bordeaux
2016-2025
Inserm
2024
Chimie et Biologie des Membranes et des Nanoobjects
2014-2022
Institut de Chimie
2016-2021
Institut de Biologie et de Chimie des Protéines
2009-2019
Université Claude Bernard Lyon 1
2009-2019
Max Planck Institute for Biophysical Chemistry
2012-2017
α-synuclein aggregation is implicated in a variety of diseases including Parkinson’s disease, dementia with Lewy bodies, pure autonomic failure and multiple system atrophy. The association protein aggregates made single clinical phenotypes has been explained for prion by the existence different strains that propagate through infection pathway. Here we structurally functionally characterize two polymorphs α-synuclein. We present evidence forms indeed fulfil molecular criteria to be identified...
One of the hallmarks Alzheimer's disease is self-assembly microtubule-associated protein tau into fibers termed "paired helical filaments" (PHFs). However, structural basis PHF assembly at atomic detail largely unknown. Here, we applied solid-state nuclear magnetic resonance (ssNMR) spectroscopy to investigate in vitro assembled PHFs from a truncated three-repeat isoform (K19) that represents core PHFs. We found rigid fibrils formed by amino acids V306 S324, only 18 out 99 residues, and...
Plasma Membrane is the primary structure for adjusting to ever changing conditions. PM sub-compartmentalization in domains thought orchestrate signaling. Yet, mechanisms governing membrane organization are mostly uncharacterized. The plant-specific REMORINs proteins regulating hormonal crosstalk and host invasion. REMs best-characterized nanodomain markers via an uncharacterized moiety called REMORIN C-terminal Anchor. By coupling biophysical methods, super-resolution microscopy physiology,...
We demonstrate sensitive detection of alpha protons fully protonated proteins by solid-state NMR spectroscopy with 100-111 kHz magic-angle spinning (MAS). The excellent resolution in the Cα-Hα plane is demonstrated for 5 proteins, including microcrystals, a sedimented complex, capsid and amyloid fibrils. A set 3D spectra based on block was developed applied sequence-specific backbone aliphatic side-chain resonance assignment using only 500 μg sample. These developments accelerate structural...
The sequence-specific resonance assignment of a protein forms the basis for studies molecular structure and dynamics, as well to functional assay by NMR spectroscopy. Here we present protocol sequential 13C 15N uniformly [15N,13C]-labeled proteins, based on suite complementary three-dimensional solid-state spectroscopy experiments. It is directed towards application proteins with more than about 100 amino acid residues. assignments rely walk along backbone using combination three experiments...
Significance Programmed cell death plays a central role in host defense plants, animals, and fungi, but the extent to which modalities are evolutionarily related mechanistically similar different kingdoms is unclear. The involvement of prion-like mechanisms cascades has been reported animals fungi. In death-inducing pore-forming protein termed HET-S activated by amyloid templating. Here we characterize HELLP, behaves analogously as membrane-targeting regulated We find that HELLP homologous...
We structurally compare, using solid-state NMR, two different polymorphs of α-synuclein which, as established recently, display contrasting biochemical properties, toxicity, and tropism for cells. show that both forms, which can each be produced a pure polymorph, are greatly in secondary structure. While β-sheets the dominating structure elements polymorphs, they markedly divergent terms number elements, well their distribution. demonstrate all identified feature an in-register parallel...
Plants respond to pathogens through dynamic regulation of plasma membrane-bound signaling pathways. To date, how the plant membrane is involved in responses viruses mostly unknown. Here, we show that cells sense Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins subsequently trigger activation a calcium-dependent kinase. We Arabidopsis thaliana CALCIUM-DEPENDENT KINASE 3-interacts with group REMORINs vivo, phosphorylates intrinsically disordered N-terminal domain Group...
While establishing an invasive infection, the dormant conidia of Aspergillus fumigatus transit through swollen and germinating stages, to form hyphae. During this morphotype transition, conidial cell wall undergoes dynamic remodeling, which poses challenges host immune system antifungal drugs. However, such reorganization during germination has not been studied so far. Here, we explored molecular rearrangement polysaccharides different stages germination. We took advantage magic-angle...
Abstract The transactive response DNA-binding protein-43 (TDP-43) is a multi-facet protein involved in phase separation, RNA-binding, and alternative splicing. In the context of neurodegenerative diseases, abnormal aggregation TDP-43 has been linked to amyotrophic lateral sclerosis frontotemporal lobar degeneration through its C-terminal domain. Here, we report cryo-electron microscopy (cryo-EM)-based structural characterization fibrils obtained from full-length protein. We find that are...
Pathogenic fungal and bacterial cells are enveloped within a cell wall, molecular barrier at their surface, critical architecture that constantly evolves during pathogenesis. Understanding the composition, structural organization, mobility of polysaccharides constituting this envelope is crucial to correlate wall organization with its role in pathogenicity identify potential antifungal targets. For pathogen Cryptococcus neoformans, characterization has been complexified by presence an...
In the fungus Podospora anserina, [Het-s] prion induces programmed cell death by activating HET-S pore-forming protein. The HET-s β-solenoid fold serves as a template for converting prion-forming domain into same fold. This conversion, in turn, activates domain. gene immediately adjacent to het-S encodes NWD2, Nod-like receptor (NLR) with an N-terminal motif similar elementary repeat unit of NLRs are immune receptors controlling and host defense processes animals, plants fungi. We have...
Stealth α-synuclein amyloids proliferate in preparations of preformed fibrils and form aggressive self-replicating strains.
The bacterial plasma membrane is an important cellular compartment. In recent years it has become obvious that protein complexes and lipids are not uniformly distributed within membranes. Current hypotheses suggest flotillin proteins required for the formation of including cell-wall synthetic proteins. We show here flotillins factors fluidity homeostasis. Loss leads to a decrease in turn alterations MreB dynamics and, as consequence, peptidoglycan synthesis. These reverted when restored by...
Significance Amyloid folds, while performing functional roles in most domains of life, remain a key factor the emergence and development multiple neurodegenerative disorders humans. The significance our study is twofold: first, by structurally characterizing highly divergent natural prion amyloids, we uncovered that amyloids can evolve regime fold conservation, withstanding extreme sequence diversification. Second, found virtually identical amyloid backbone structures might not be sufficient...
Significance Bactofilins are a new class of cytoskeletal proteins that involved in key cellular processes. For instance, the human pathogen Helicobacter pylori , they responsible for maintaining its characteristic helical cell shape, feature required cells to efficiently colonize gastric mucus. So far atomic structure bactofilin filaments has remained elusive, as large assemblies not amenable standard methods 3D determination. Here, we have applied combination solid-state NMR and electron...
Abstract Nanodiscs offer a very promising tool to incorporate membrane proteins into native‐like lipid bilayers and an alternative liposomes maintain protein functions protein–lipid interactions in soluble nanoscale object. The activity of the incorporated appears be correlated its dynamics bilayer by interactions. These two parameters depend on internal surrounded lipid‐encircling discoidal scaffold that might differ from more unrestricted observed vesicles or cellular extracts. A...
The formation of biofilms provides structural and adaptive bacterial response to the environment. In Bacillus species, biofilm extracellular matrix is composed exopolysaccharides, hydrophobins, several functional amyloid proteins. We report, using multiscale approaches such as solid-state NMR (SSNMR), electron microscopy, X-ray diffraction, dynamic light scattering, attenuated total reflection Fourier transform infrared (FTIR), immune-gold labeling, molecular architecture B. subtilis...
The Type Three Secretion System (T3SS) is a macromolecular protein nano-syringe used by different bacterial pathogens to inject effectors into host cells. extracellular part of the syringe needle-like filament formed polymerization 9-kDa whose structure and proper localization on surface are key determinants for efficient toxin injection. Here, we combined in vivo, vitro, silico approaches characterize Pseudomonas aeruginosa T3SS needle its major component PscF. Using combination...