A5062308894- Protein Structure and Dynamics
- Enzyme Structure and Function
- Advanced NMR Techniques and Applications
- Bacteriophages and microbial interactions
- Reproductive tract infections research
- Solid-state spectroscopy and crystallography
- Bacterial Genetics and Biotechnology
- Advanced Proteomics Techniques and Applications
- Plant-Microbe Interactions and Immunity
- Alzheimer's disease research and treatments
- Plant Pathogens and Resistance
- Crystallography and Radiation Phenomena
- Urinary Tract Infections Management
- Scientific Computing and Data Management
- Supramolecular Self-Assembly in Materials
- Prion Diseases and Protein Misfolding
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Escherichia coli research studies
- Plant Parasitism and Resistance
- Peptidase Inhibition and Analysis
- Plant and fungal interactions
- NMR spectroscopy and applications
- Protein Tyrosine Phosphatases
- Veterinary medicine and infectious diseases
University of East Anglia
2012-2025
Norwich Research Park
2018-2025
European Bioinformatics Institute
2019-2024
Université Claude Bernard Lyon 1
2023-2024
Worldwide Protein Data Bank
2024
Institut Polytechnique de Bordeaux
2016-2023
Université de Bordeaux
2016-2023
Centre National de la Recherche Scientifique
2016-2023
Institut Européen de Chimie et Biologie
2017-2023
Centre de RMN à Très Hauts Champs de Lyon
2023
Abstract The Protein Data Bank in Europe (PDBe), a founding member of the Worldwide (wwPDB), actively participates deposition, curation, validation, archiving and dissemination macromolecular structure data. PDBe supports diverse research communities their use structures by enriching PDB data providing advanced tools services for effective access, visualization analysis. This paper details enrichment at PDBe, including mapping RNA to Rfam, identification molecules that act as cofactors. has...
While establishing an invasive infection, the dormant conidia of Aspergillus fumigatus transit through swollen and germinating stages, to form hyphae. During this morphotype transition, conidial cell wall undergoes dynamic remodeling, which poses challenges host immune system antifungal drugs. However, such reorganization during germination has not been studied so far. Here, we explored molecular rearrangement polysaccharides different stages germination. We took advantage magic-angle...
Abstract The archiving and dissemination of protein nucleic acid structures as well their structural, functional biophysical annotations is an essential task that enables the broader scientific community to conduct impactful research in multiple fields life sciences. Protein Data Bank Europe (PDBe; pdbe.org ) team develops maintains several databases web services address this fundamental need. From data a member Worldwide PDB consortium (wwPDB; wwpdb.org ), PDBe Knowledge Base (PDBe‐KB;...
ModelCIF (github.com/ihmwg/ModelCIF) is a data information framework developed for and by computational structural biologists to enable delivery of Findable, Accessible, Interoperable, Reusable (FAIR) users worldwide. describes the specific set attributes metadata associated with macromolecular structures modeled solely methods provides an extensible representation deposition, archiving, public dissemination predicted three-dimensional (3D) models macromolecules. It extension Protein Data...
The formation of biofilms provides structural and adaptive bacterial response to the environment. In Bacillus species, biofilm extracellular matrix is composed exopolysaccharides, hydrophobins, several functional amyloid proteins. We report, using multiscale approaches such as solid-state NMR (SSNMR), electron microscopy, X-ray diffraction, dynamic light scattering, attenuated total reflection Fourier transform infrared (FTIR), immune-gold labeling, molecular architecture B. subtilis...
The Type Three Secretion System (T3SS) is a macromolecular protein nano-syringe used by different bacterial pathogens to inject effectors into host cells. extracellular part of the syringe needle-like filament formed polymerization 9-kDa whose structure and proper localization on surface are key determinants for efficient toxin injection. Here, we combined in vivo, vitro, silico approaches characterize Pseudomonas aeruginosa T3SS needle its major component PscF. Using combination...
Oomycete pathogens cause large economic losses in agriculture through diseases such as late blight (Phytophthora infestans), and stem root rot of soybean sojae). The effector protein AVR3a, from P. infestans, its homologue AVR3a11 Phytophthora capsici, are host-translocated effectors that interact with plant proteins to evade defense mechanisms enable infection. Both belong the family RXLR contain an N-terminal secretion signal, motif for translocation into host cell, a C-terminal domain....
Biomolecular structure analysis from experimental NMR studies generally relies on restraints derived a combination of and knowledge-based data. A challenge for the structural biology community has been lack standards representing these restraints, preventing establishment uniform methods model-vs-data validation against limiting interoperability between restraint-based modeling programs. The NEF NMR-STAR formats provide standardized approach commonly used restraints. Using restraint formats,...
Type III protein-secretion machines are essential for the interactions of many pathogenic or symbiotic bacterial species with their respective eukaryotic hosts. The core component these is injectisome, a multiprotein complex that mediates selection substrates, passage through envelope, and ultimately delivery into target cells. injectisome composed large cytoplasmic sorting platform, multiring base embedded in needle-like filament protrudes several nanometers from surface capped at its...
More than 61,000 proteins have up-to-date correspondence between their amino acid sequence (UniProtKB) and 3D structures (PDB), enabled by the Structure Integration with Function, Taxonomy Sequences (SIFTS) resource. SIFTS incorporates residue-level annotations from many other biological resources. data is available in various formats like XML, CSV TSV format or also accessible via PDBe REST API but always maintained separately structure (PDBx/mmCIF file) PDB archive. Here, we extended wwPDB...
Abstract Under appropriate conditions, peptides and proteins can assemble from their native state into prefibrillar oligomers then mature fibrillar aggregates. This transition forms the molecular basis of several pathologies, often related to deposition these amyloid fibrils. Several hormone involved in fundamental biological processes have tendency self‐assemble fibrils, resulting a loss functions, more importantly, entailing devastating consequences, such as formation depositions....
Tarp (translocated actin recruiting phosphoprotein) is an effector protein common to all chlamydial species that functions remodel the host-actin cytoskeleton during initial stage of infection. In C. trachomatis, direct binding monomers has been broadly mapped a 100-residue region (726-825) which predicted be predominantly disordered, with exception ~10-residue α-helical patch homologous other WH2 actin-binding motifs. Biophysical investigations demonstrate Tarp726-825 construct behaves as...
WWP2 is an E3 ubiquitin ligase that differentially regulates the contextual tumour suppressor/progressor TGFβ signalling pathway by alternate isoform expression. isoforms select signal transducer Smad2/3 or inhibitor Smad7 substrates for degradation through different compositions of protein-protein interaction WW domains. The WW4 domain-containing WWP2-C induces turnover in vivo and positively metastatic epithelial-mesenchymal transition programme. This activity overexpression these human...
We have recently reported on the preparation of a membrane-associated β-barrel Pore-Forming Aβ42 Oligomer (βPFOAβ42). It corresponds to stable and homogeneous oligomer that inserts into lipid bilayers as well-defined pore adopts specific structure with characteristics arrangement. As follow-up this work, we aim establish βPFOAβ42's relevance in Alzheimer's disease (AD). However, βPFOAβ42 is formed under dodecyl phosphocholine (DPC) micelle conditions—intended mimic hydrophobic environment...
(+)-2,3-trans-3,4-cis-Leucocyanidin was produced by acidic epimerization of (+)-2,3-trans-3,4-trans-leucocyanidin synthesized reduction (+)-dihydroquercetin with NaBH4, and structures the two stereoisomers purified C18- phenyl-reverse-phase high-performance liquid chromatography (HPLC) were confirmed NMR spectroscopy. We confirm that only 3,4-cis-leucocyanidin is used leucoanthocyanidin reductase as substrate. The are quite stable in aqueous solution at -20 °C. Characterization also...
Somatostatin (SST14) is strongly related to Alzheimer's disease (AD), as its levels decline during aging, it regulates the proteolytic degradation of amyloid beta peptide (Aβ), and binds Aβ oligomers in vivo. Recently, 3D structure a membrane-associated β-sheet pore-forming tetramer (βPFOAβ(1–42) tetramer) has been reported. Here, we show that SST14 selectively βPFOAβ(1–42) with KD value ∼40 μM without binding monomeric Aβ(1–42). Specific NMR chemical shift perturbations, observed titration...
Abstract Structural investigations of amyloid fibrils often rely on heterologous bacterial overexpression the protein interest. Due to their inherent hydrophobicity and tendency aggregate as inclusion bodies, many proteins are challenging express in systems. Cell-free expression is a promising alternative classical produce hydrophobic introduce NMR-active isotopes that can improve speed up NMR analysis. Here we implement cell-free synthesis functional prion HET-s(218-289). We present an...
Phosphoinositide lipids recruit proteins to the plasma membrane involved in regulation of cytoskeleton organization and signalling pathways that control cell polarity growth. Among those, Rgd1p is a yeast GTPase-activating protein (GAP) specific for Rho3p Rho4p GTPases, which actin polymerization stress pathways. Phosphoinositides not only bind Rgd1p, but also stimulate its GAP activity on membrane-anchored form Rho4p. Both F-BAR (F-BAR FCH, BAR) RhoGAP domains are lipid interactions. In...
Summary Biomolecular structure analysis from experimental NMR studies generally relies on restraints derived a combination of and knowledge-based data. A challenge for the structural biology community has been lack standards representing these restraints, preventing establishment uniform methods model-vs-data validation against limiting interoperability between restraint-based modeling programs. The exchange (NEF) NMR-STAR formats provide standardized approach commonly used restraints. Using...
Abstract Oomycete pathogens cause large economic losses in agriculture through diseases such as late blight ( Phytophthora infestans ), and stem root rot of soybean sojae ). The effector protein AVR3a, from P. , its homologue AVR3a11 capsici are examples host-translocated effectors that interact with plant proteins to evade defence mechanisms enable infection. Both belong the family RXLR contain an N-terminal secretion signal, motif for translocation into host cell, a C-terminal domain....
Dissolution dynamic nuclear polarization has emerged as a powerful technique to address the inherent sensitivity limitations in magnetic resonance imaging and spectroscopy. The relies on use of stable paramagnetic polarizing agents that act source hyperpolarization but unfortunately their presence contaminates sample significantly enhances relaxation hyperpolarized solutions. In last decade, novel formulations have been proposed combat this issue, including silica-based hybrid solids...