A5054736022- Bacteriophages and microbial interactions
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Polyomavirus and related diseases
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Cellular Mechanics and Interactions
- Protist diversity and phylogeny
- Biochemical and Structural Characterization
- Proteins in Food Systems
- Food composition and properties
- Genetic and Kidney Cyst Diseases
- Plant Virus Research Studies
- Salmonella and Campylobacter epidemiology
- Advanced Electron Microscopy Techniques and Applications
- Erythrocyte Function and Pathophysiology
- RNA Interference and Gene Delivery
- Photosynthetic Processes and Mechanisms
- Sulfur Compounds in Biology
- Neonatal and Maternal Infections
- Immune Response and Inflammation
- Bone Tissue Engineering Materials
- Nitric Oxide and Endothelin Effects
- Infections and bacterial resistance
Charles University
2000-2024
Agency for Science, Technology and Research
2016-2022
Pathogénèse Bactérienne et Réponses Cellulaires
2015-2019
Inserm
2015-2019
Centre National de la Recherche Scientifique
2015-2019
CEA Grenoble
2014-2019
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2014-2019
Université Grenoble Alpes
2015-2019
The University of Queensland
2013-2017
Institute of Medical Biology
2016
It is well established that Ly6Chi monocytes develop from common monocyte progenitors (cMoPs) and reside in the bone marrow (BM) until they are mobilized into circulation. In our study, we found BM not a homogenous population, as current data would suggest. Using computational analysis approaches to interpret multidimensional datasets, demonstrate consist of two distinct subpopulations (CXCR4hi CXCR4lo subpopulations) both mice humans. Transcriptome studies vivo assays revealed functional...
Motile cilia defects impair cerebrospinal fluid (CSF) flow and can cause brain spine disorders. The development of ciliated cells, their impact on CSF flow, function in axial morphogenesis are not fully understood. We have characterized motile cells within the zebrafish ventricles. show that ventricles undergo restructuring through development, involving a transition from mono- to multiciliated (MCCs) driven by gmnc. MCCs co-exist with monociliated generate directional patterns. These...
ABSTRACT Electron and confocal microscopy were used to observe the entry movement of polyomavirus virions artificial virus-like particles (VP1 pseudocapsids) in mouse fibroblasts epithelial cells. No visible differences adsorption internalization VP1 pseudocapsids (“empty” or containing DNA) observed. Viral entered cells internalized smooth monopinocytic vesicles, often proximity larger, caveola-like invaginations. Both “empty” vesicles derived from caveolae viral stained with...
Dynamic remodelling of the cortical actin cytoskeleton is required for phagocytic uptake pathogens and other particles by macrophages. Actin can also be nucleated de novo on membranes nascent phagosomes, a process that stimulate or inhibit phagosome fusion with lysosomes. Recently, phagosomes were shown to polymerize in transient pulses, called ;flashing', whose function remains unexplained. Here, we investigated phagosomal dynamics live macrophages expressing tagged green fluorescent...
During the intracellular life of Salmonella enterica, a unique membrane-bound compartment termed Salmonella-containing vacuole, or SCV, is formed. By means translocated effector proteins, also induce formation extensive, highly dynamic membrane tubules Salmonella-induced filaments SIF. Here we report first detailed ultrastructural analyses SCV and SIF by electron microscopy (EM), EM tomography live cell correlative light (CLEM). We found that subset composed double membranes enclose portions...
The infection by Salmonella enterica results in the massive remodeling of endosomal system eukaryotic host cells. One unique consequence is formation long tubular compartments, so-called Salmonella-induced filaments (SIF). Formation SIF requires function type III secretion and a requirement efficient intracellular proliferation Salmonella. Using high-resolution live cell imaging approaches electron microscopy, we report for first time highly dynamic characteristics their ultrastructural...
Sortilin, also known as neurotensin receptor 3 (NTR3), is a transmembrane protein with dual function. It acts for neuromediators and growth factors at the plasma membrane, but it has been implicated in binding transport of some lysosomal proteins. However, role sortilin during phagosome maturation not investigated before. Here, we show that macrophages, mainly localized Golgi transported to latex-bead phagosomes (LBPs). Using live-cell imaging electron microscopy, found delivered LBPs manner...
In contrast to phagocytosis, macropinocytosis is not directly initiated by interactions between cell surface receptors and cargo ligands, but a result of constitutive membrane ruffling driven dynamic remodelling cortical actin cytoskeleton in response stimulation growth factor receptors. Wang et al. (2010) [13] developed reliable assay that allows quantitative assessment the efficiency kinetics macropinosome biogenesis and/or maturation cells where function targeted protein has been...
The Type Three Secretion System (T3SS) is a macromolecular protein nano-syringe used by different bacterial pathogens to inject effectors into host cells. extracellular part of the syringe needle-like filament formed polymerization 9-kDa whose structure and proper localization on surface are key determinants for efficient toxin injection. Here, we combined in vivo, vitro, silico approaches characterize Pseudomonas aeruginosa T3SS needle its major component PscF. Using combination...
Abstract Hutchinson–Gilford progeria is a premature aging syndrome caused by truncated form of lamin A called progerin. Progerin expression results in variety cellular defects including heterochromatin loss, DNA damage, impaired proliferation and senescence. It remains unclear how these different progerin‐induced phenotypes are temporally mechanistically linked. To address questions, we use doxycycline‐inducible system to restrict progerin stages the cell cycle. We find that leads rapid...
Abstract Correlative light and electron microscopy (CLEM) is an important tool for the localisation of target molecule(s) their spatial correlation with ultrastructural map subcellular features at nanometre scale. Adoption these advanced imaging methods has been limited in plant biology, due to challenges tissue permeability, fluorescence labelling efficiency, indexing interest throughout complex 3D volume re-localization on micrographs ultrathin cross-sections. Here, we demonstrate approach...
ABSTRACT Mouse polyomavirus (PyV) virions enter cells by internalization into smooth monopinocytic vesicles, which fuse under the cell membrane with larger endosomes. Caveolin-1 was detected on vesicles carrying PyV particles in mouse fibroblasts and epithelial (33). Here, we show that can be efficiently internalized Jurkat cells, do not express caveolin-1 lack caveolae, overexpression of a dominant-negative mutant does prevent their productive infection. Strong colocalization VP1 early...
Intracellular Salmonella enterica induce a massive remodeling of the endosomal system in infected host cells. One dramatic consequence this interference is induction various extensive tubular aggregations membrane vesicles, and tubules positive for late endosomal/lysosomal markers are referred to as Salmonella-induced filaments or SIF. SIF highly dynamic nature with extension collapse velocities 0.4–0.5 µm x sec−1. The depends on function Pathogenicity Island 2 (SPI2) encoded type III...
To invade epithelial cells, Salmonella enterica serovar Typhimurium (S. Typhimurium) induces macropinocytosis through the action of virulence factors delivered across host cell membrane via a type III secretion system. We show that after docking at plasma S. triggers rapid recruitment cytosolic SNX18, SH3-PX-BAR domain sorting nexin protein, to bacteria-induced ruffles and nascent Salmonella-containing vacuole. SNX18 required inositol-phosphatase activity effector SopB an intact...
In this study, spherical or hexagonal NaYF4:Yb,Er nanoparticles (UCNPs) with sizes of 25 nm (S-UCNPs) and 120 (L-UCNPs) were synthesized by high-temperature coprecipitation subsequently modified three kinds polymers. These included poly(ethylene glycol) (PEG) poly(N,N-dimethylacrylamide-co-2-aminoethylacrylamide) [P(DMA-AEA)] terminated an alendronate anchoring group, poly(methyl vinyl ether-co-maleic acid) (PMVEMA). The internalization rat mesenchymal stem cells (rMSCs) C6 cancer (rat glial...
A vector for preparation of mouse polyomavirus capsid‐like particles transfer foreign peptides or proteins into cells was constructed. Model pseudocapsids carrying EGFP fused with the C‐terminal part VP3 minor protein (EGFP‐VLPs) have been prepared and analysed their ability to be internalised processed by activate human dendritic (DC) in vitro. EGFP‐VLPs entered epithelial cells, fibroblasts DC efficiently were both, lysosomes proteasomes. Surprisingly, they did not induce upregulation...
Desmosomes, strong cell-cell junctions of epithelia and cardiac muscle, link intermediate filaments to cell membranes mechanically integrate cells across tissues, dissipating mechanical stress. They comprise five major protein classes - desmocollins desmogleins (the desmosomal cadherins), plakoglobin, plakophilins desmoplakin whose individual contribution the structure turnover desmosomes is poorly understood. Using live-cell imaging together with fluorescence recovery after photobleaching...
ABSTRACT BK polyomavirus (BKPyV) infection in humans is usually asymptomatic but ultimately results viral persistence. In immunocompromised hosts, virus reactivation can lead to nephropathy or hemorrhagic cystitis. The urinary tract serves as a silent reservoir for the virus. Recently, it has been demonstrated that human bladder microvascular endothelial cells (HBMVECs) serve reservoirs, given their unique response infection, which involves interferon (IFN) production. aim of present study...
Polyomavirus mutants E, Q and H, expressing non-myristylated VP2, were generated by replacing the N-terminal glycine residue with glutamic acid, glutamine or histidine, respectively. Viruses mutated in either VP2 VP3 translation initiation codons also prepared. All genomes, when transfected into murine host cells, gave rise to viral particles. Infectivity of VP2- VP3- viruses, as measured number cells antigens, was dramatically diminished, indicative defects early stages infection. In...
The export of bacterial toxins across the envelope requires assembly complex, membrane-embedded protein architectures. Pseudomonas aeruginosa employs type III secretion (T3S) injectisome to translocate exotoxins directly into cytoplasm a target eukaryotic cell. This multi-protein channel crosses two membranes and extends further as needle through which proteins travel. We show in this work that PscI, proposed form T3S system (T3SS) inner rod, possesses intrinsic properties polymerize...
The Gram-negative bacteria use the contractile multi-molecular structure, called Type VI Secretion System (T6SS) to inject toxic products into eukaryotic and prokaryotic cells. In this study, we fluorescent protein fusions time-lapse microscopy imaging study assembly dynamics of baseplate TssK in Pseudomonas aeruginosa T6SS. formed transient higher-order structures that correlated with sheath component TssB. Assembly peri-membrane occurred de novo upon contact competing bacteria. We show...