A5060005822- Immune cells in cancer
- Asthma and respiratory diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Viral Infections and Vectors
- Chemokine receptors and signaling
- Mosquito-borne diseases and control
- Single-cell and spatial transcriptomics
- Immune responses and vaccinations
- Phagocytosis and Immune Regulation
- T-cell and B-cell Immunology
- Allergic Rhinitis and Sensitization
- RNA Interference and Gene Delivery
- Eosinophilic Esophagitis
- IL-33, ST2, and ILC Pathways
- Neonatal Respiratory Health Research
- Cancer Cells and Metastasis
- Infant Nutrition and Health
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Dermatology and Skin Diseases
Singapore Immunology Network
2016-2024
Agency for Science, Technology and Research
2016-2024
National University of Singapore
2014-2019
Singapore-HUJ Alliance for Research and Enterprise
2019
National University Health System
2016
Neutrophils are increasingly recognized as key players in the tumor immune response and associated with poor clinical outcomes. Despite recent advances characterizing diversity of neutrophil states cancer, common trajectories mechanisms governing ontogeny relationship between these remain undefined. Here, we demonstrate that immature mature neutrophils enter tumors undergo irreversible epigenetic, transcriptional, proteomic modifications to converge into a distinct, terminally differentiated...
It is well established that Ly6Chi monocytes develop from common monocyte progenitors (cMoPs) and reside in the bone marrow (BM) until they are mobilized into circulation. In our study, we found BM not a homogenous population, as current data would suggest. Using computational analysis approaches to interpret multidimensional datasets, demonstrate consist of two distinct subpopulations (CXCR4hi CXCR4lo subpopulations) both mice humans. Transcriptome studies vivo assays revealed functional...
Little is known about the cellular mechanisms of innate immunity against dengue virus (DV) infection. Specifically, γδ T cell response to DV has not been characterized in detail. In this article, we demonstrate that markers activation, proliferation, and degranulation are upregulated on cells PBMC isolated from individuals with acute fever. Primary responded rapidly vitro autologous DV-infected dendritic by secreting IFN-γ upregulating CD107a. The anti-DV regulated type I IFN IL-18 a...
Circulating Ly6C hi monocytes often undergo cellular death upon exhaustion of their antibacterial effector functions, which limits capacity for subsequent macrophage differentiation. This shrouds the understanding on how host replaces tissue-resident niche effectively during bacterial invasion to avert infection morbidity. Here, we show that proliferating transitional premonocytes (TpMos), an immediate precursor mature (MatMos), were mobilized into periphery in response acute and sepsis....
Clinical studies have suggested the importance of NK cell response against dengue virus (DenV), an arboviral infection that afflicts >50 million individuals each year. However, a comprehensive understanding dengue-infected cells is lacking. To characterize cell-contact mechanisms and soluble factors contribute to antidengue response, primary human were cocultured with autologous DenV-infected monocyte-derived dendritic (DC). responded by cytokine production lysis target cells. Notably, in...
Abstract Previous studies have highlighted the importance of lung-draining lymph nodes in respiratory allergic immune response, whereas lung parenchymal system has been largely neglected. We describe a new vivo model sensitization to Blomia tropicalis, principal asthma allergen tropics, which response is focused on parenchyma by transfer Th2 cells from novel TCR transgenic mouse, specific for major B. tropicalis Blo t 5, that targets rather than draining nodes. Transfer highly polarized CD4...
Summary Sensitization of allergic patients normally takes place over several years and is the result repeated exposure to low levels allergen. Most mouse asthma models use a high dose allergen administered short period. We have investigated role in immune response an inhaled respiratory ( Blomia tropicalis ). observed effect priming on mice intranasally immunized with (0·5 μg) (50 doses B. extract killed 1 day after last challenge. For both allergen, T helper type 2 (Th2) cells Th2 cytokines...
The significance of intracellular Ap4A levels over immune activity dendritic cells (DCs) has been studied in Nudt2fl/fl/CD11c-cre mice. transgenic mice have generated by crossing floxed NUDT2 gene with DC marker CD11c recombinase (cre) DCs derived from these higher (≈30-fold) compared those Nudt2+/+ Interestingly, the elevated led them to possess motility and lower directional variability. In addition, are able enhance protection indicated cross-presentation antigen priming CD8+ OT-I T...
Neutrophils are essential immune cells that defend the host against pathogenic microbial agents. produced in bone marrow and retained there through CXCR4-CXCL12 signaling. However, patients with Warts, Hypogammaglobulinemia, Infections, Myelokathexis (WHIM) syndrome prone to infections due increased accumulation of neutrophils leading low numbers circulating neutrophils. How accumulate this condition is poorly understood. To better understand factors involved neutrophil marrow, from wildtype...
Abstract Patients with severe asthma are very difficult to manage present a major medical challenge. Development of clinical requires many years patient exposure allergens. Mouse models require weeks and normally transitory do not lead allergen-induced dyspnea. We have followed an alternative approach conventional immunization through the transfer highly polarized CD4 T cell receptor transgenic cells specific for dust mite allergen Blo t 5 by thrice weekly intranasal recombinant challenge...