A5027533651- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Immune cells in cancer
- Mast cells and histamine
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Lymphoma Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Oral microbiology and periodontitis research
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Pediatric health and respiratory diseases
- Influenza Virus Research Studies
- Respiratory viral infections research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Immunotherapy and Biomarkers
- Polydiacetylene-based materials and applications
- Viral-associated cancers and disorders
- Nosocomial Infections in ICU
- Retinoids in leukemia and cellular processes
- Biotin and Related Studies
Singapore Immunology Network
2015-2024
Agency for Science, Technology and Research
2015-2024
National Cancer Centre Singapore
2008
University of Iowa
2007
Abstract This study sought to identify critical determinants of mesenchymal stem cell (MSC) potency using in vitro and vivo attributes cells isolated from the bone marrow age- sex-matched donors. Adherence plastic was not indicative potency, yet capacity for long-term expansion varied considerably between donors, allowing grouping MSCs donors into either those with high-growth or low-growth capacity. Using this strategy, were smaller size, had greater colony-forming efficiency, longer...
SARS-CoV-2 is the novel coronavirus responsible for current COVID-19 pandemic. Severe complications are observed only in a small proportion of infected patients but cellular mechanisms underlying this progression still unknown. Comprehensive flow cytometry whole blood samples from 54 reveals dramatic increase number immature neutrophils. This strongly correlates with disease severity and associated elevated IL-6 IP-10 levels, two key players cytokine storm. The most pronounced decrease cell...
Abstract Background Southeast Asian populations are increasingly affected by allergic airway diseases. Etiology and specific causes, however, still unknown. The aim of this study is therefore to identify allergens risk factors for the high prevalence disease in tropical urban environment. Methods Symptoms rhinitis (AR), asthma, dermatitis were recorded two independent cohorts 576 7373 ethnic Chinese individuals living Singapore. Reactivity against common was determined skin prick tests...
Little is known about the cellular mechanisms of innate immunity against dengue virus (DV) infection. Specifically, γδ T cell response to DV has not been characterized in detail. In this article, we demonstrate that markers activation, proliferation, and degranulation are upregulated on cells PBMC isolated from individuals with acute fever. Primary responded rapidly vitro autologous DV-infected dendritic by secreting IFN-γ upregulating CD107a. The anti-DV regulated type I IFN IL-18 a...
Human Vγ2Vδ2 T cells are stimulated by prenyl pyrophosphates, such as isopentenyl pyrophosphate (IPP), and play important roles in mediating immunity against microbial pathogens have potent anti-tumor activity. (E)-4-hydroxy-3-methyl-but-2-enyl (HMBPP) has been identified a metabolite the 2-C-methyl-D-erythritol-4 phosphate (MEP) pathway for isoprenoid biosynthesis that is used many bacteria protozoan parasites. We find HMBPP major T-cell antigen bacteria, including Mycobacterium...
Abstract Monocytes play a central role in regulating inflammation response to infection or injury, and during auto-inflammatory diseases. Human blood contains classical, intermediate non-classical monocyte subsets that each express characteristic patterns of cell surface CD16 CD14; subset also has specific functional properties, but the mechanisms underlying many their distinctive features are undefined. Of particular interest is how regulate secretion apical pro-inflammatory cytokine IL-1β,...
Abstract Aging is associated with an increased susceptibility to infections and diseases. It has also been reduced functionality altered distribution of immune cells, especially T cells. Whereas classical α/β CD8+ were shown be highly susceptible aging, the effects viral persistent stimulations on fate γ/δ cells are much less documented. Healthy, elderly individuals Chinese ethnical background recruited under aegis SLAS-II. In this observational study, cell populations characterized by flow...
Cohort studies indicated that in certain individuals the basophils do not respond toward allergens due to a desensitization of their Fc epsilon receptor pathway. Cause and functional role as well implications on allergic reactions, however, are clear yet.A cross-sectional study was carried out tropical urban environment Singapore, where response is dominated by single allergen (house dust mite; HDM). Blood samples were collected from 476 analyzed comprehensively correlate state with clinical...
Expression quantitative trait loci (eQTL) databases represent a valuable resource to link disease-associated SNPs specific candidate genes whose gene expression is significantly modulated by the SNP under investigation. We previously identified signal inhibitory receptor on leukocytes-1 (SIRL-1) as powerful regulator of human innate immune cell function. While it constitutively high expressed neutrophils, monocytes SIRL-1 surface varies strongly between individuals. The underlying mechanism...
Extracellular ATP is a pro-inflammatory molecule released by damaged cells. Regulatory T cells (Treg) can suppress inflammation hydrolysing this via ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1), also termed as CD39. Multiple studies have reported differences in CD39+ Treg percentages diseases such multiple sclerosis, Hepatitis B and HIV-1. In addition, CD39 polymorphisms been implicated immune-phenotypes susceptibility to inflammatory bowel disease AIDS progression. However none...
Abstract Delta-like ligand 3 (DLL3) is a promising target for antibody-drug conjugates (ADCs) in the treatment of small cell lung cancer (SCLC) due to its high expression on surface SCLC cells and minimal normal tissues. With highly specific, fast internalized mAbs from antibody screening, various types payloads including tubulin TOPI inhibitors were conjugated with different platforms, linkers, conjugation methods available our linker-payload arsenal obtain optimal ADC. Here, we present...
Abstract Background: Claudin 6 (CLDN6) is a clinically validated target for many solid tumors notably ovarian, endometrial, and testicular cancer. Its expression restricted to tumor cells fetal tissues with little or no detectable in normal tissues. As such, CLDN6 represents an attractive therapeutic the development of antibody drug conjugate (ADC). Here, we describe preclinical characterization novel ADC called PLB-002, which consists highly selective targeting antibody, PLB-002-ab7,...
Neutrophils are an abundant immune cell type involved in both antimicrobial defence and autoimmunity. The regulation of their gene expression, however, is still largely unknown. Here we report eQTL study on isolated neutrophils from 114 healthy individuals Chinese ethnicity, identifying 21,210 eQTLs 832 unique genes. Unsupervised clustering analysis these confirms role inflammatory responses immunological diseases but also indicates strong involvement dermatological pathologies. One the...
Background: We established an in vitro co-culture model involving H3N2-infection of human nasal epithelium with peripheral blood mononuclear cells (PBMC) to investigate their cross-talk during early H3N2 infection. Methods: Nasal was differentiated from epithelial stem/progenitor and cultured wtih fresh PBMC. PBMC supernatants were harvested after 24h 48h H3N2-infected epithelium. used flow cytometry Luminex characterize subpopulations, activation cytokines chemokines secretion. Results:...
// Crystal Tze Ying Tan 1 , Kilian Wistuba-Hamprecht 2,3 Weili Xu 1,4 Ma Schwe Zin Nyunt 5 Anusha Vasudev Bernett Teck Kwong Lee Graham Pawelec 2 Kia Joo Puan Olaf Rotzschke Pin Ng and Anis Larbi 1,4,6 Singapore Immunology Network (SIgN), Agency for Science Technology Research (A*STAR), Biopolis, Department of Internal Medicine II, Centre Medical Research, University Center, Tübingen, Germany 3 Dermatology, 4 School Biological Sciences, Nanyang Technological University, Gerontology...
Abstract Resistin is a key cytokine associated with metabolic and inflammatory diseases. Especially in East Asian populations, the expression levels are strongly influenced by genetic polymorphisms. Mechanisms functional implications of this control still unknown. By employing reporter assays, EMSA, inhibition studies, bisulphite sequencing, ChIP-Seq gene-editing we show that p50/p50 homodimer known to act as repressor for number pro-inflammatory genes plays central role regulation resistin...
Severe SARS-CoV-2 infection can trigger uncontrolled innate and adaptive immune responses, which are commonly associated with lymphopenia increased neutrophil counts. However, whether the abnormalities observed in mild to severely infected patients persist into convalescence remains unclear. Herein, comparisons were drawn between responses of COVID-19 convalescent adults. Strikingly, survivors severe had decreased proportions NKT Vδ2 T cells, low-density neutrophils, IgA+/CD86+/CD123+...
With lowering costs of sequencing and genetic profiling techniques, drivers can now be detected readily in tumors but current prognostic models for Natural-killer/T cell lymphoma (NKTCL) have yet to fully leverage on them prognosticating patients. Here, we used next-generation sequence 260 NKTCL tumors, trained a genomic model (GPM) with the mutations survival data from this retrospective cohort patients using LASSO Cox regression. The GPM is defined by mutational status 13 genes weakly...
Adoptive cell therapy is an emerging treatment strategy for a number of serious diseases. Regulatory T (Treg) cells represent 1 type particular interest inflammatory conditions, as they are responsible controlling unwanted immune responses. Initial clinical trials adoptive transfer Treg in patients with graft-versus-host disease were shown to be safe. However, obtaining sufficient numbers highly pure and functional minimal contamination remains challenge. We developed novel approach isolate...
Abstract Sialyl-Lewis x (sLe , CD15s) is a tetra-saccharide on the surface of leukocytes required for E-selectin-mediated rolling, prerequisite to migrate out blood vessels. Here we show using flow cytometry that sLe expression basophils and mast cell progenitors depends fucosyltransferase 6 ( FUT6 ). Using genetic association data analysis qPCR, type-specific defect was associated with single nucleotide polymorphisms (SNPs) in gene region (tagged by rs17855739 rs778798), affecting coding...
Abstract SARS-CoV-2 is the novel coronavirus responsible for current COVID-19 pandemic. Severe complications are observed only in a small proportion of infected patients but cellular mechanisms underlying this progression still unknown. Comprehensive flow cytometry whole blood samples from 54 revealed dramatic increase number immature neutrophils. This strongly correlated with disease severity and was associated elevated IL-6 IP-10 levels, two key players cytokine storm. The most pronounced...