Immanuel Kwok
A5046835402- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Single-cell and spatial transcriptomics
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- T-cell and B-cell Immunology
- Cell Image Analysis Techniques
- Neonatal Respiratory Health Research
- COVID-19 Clinical Research Studies
- Microfluidic and Bio-sensing Technologies
- Immunotherapy and Immune Responses
- Neuroinflammation and Neurodegeneration Mechanisms
- IL-33, ST2, and ILC Pathways
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Luminescence and Fluorescent Materials
- Blood disorders and treatments
- Chemokine receptors and signaling
- Acute Myeloid Leukemia Research
- Cancer Cells and Metastasis
- Invertebrate Immune Response Mechanisms
- Sulfur-Based Synthesis Techniques
- Molecular Communication and Nanonetworks
- Telomeres, Telomerase, and Senescence
- Hematopoietic Stem Cell Transplantation
Singapore Immunology Network
2017-2025
Agency for Science, Technology and Research
2017-2025
Nanyang Technological University
2018-2021
SARS-CoV-2 is the novel coronavirus responsible for current COVID-19 pandemic. Severe complications are observed only in a small proportion of infected patients but cellular mechanisms underlying this progression still unknown. Comprehensive flow cytometry whole blood samples from 54 reveals dramatic increase number immature neutrophils. This strongly correlates with disease severity and associated elevated IL-6 IP-10 levels, two key players cytokine storm. The most pronounced decrease cell...
Neutrophils are increasingly recognized as key players in the tumor immune response and associated with poor clinical outcomes. Despite recent advances characterizing diversity of neutrophil states cancer, common trajectories mechanisms governing ontogeny relationship between these remain undefined. Here, we demonstrate that immature mature neutrophils enter tumors undergo irreversible epigenetic, transcriptional, proteomic modifications to converge into a distinct, terminally differentiated...
Advances in spatial omics technologies now allow multiple types of data to be acquired from the same tissue slice. To realize full potential such data, we need spatially informed methods for integration. Here, introduce SpatialGlue, a graph neural network model with dual-attention mechanism that deciphers domains by intra-omics integration location and measurement followed cross-omics We demonstrated SpatialGlue on different using technologies, including epigenome-transcriptome...
Tumor-associated macrophages (TAMs) are a heterogeneous population of cells whose phenotypes and functions shaped by factors that incompletely understood. Herein, we asked when where TAMs arise from blood monocytes how they evolve during tumor development. We initiated pancreatic ductal adenocarcinoma (PDAC) in inducible monocyte fate-mapping mice combined single-cell transcriptomics high-dimensional flow cytometry to profile the monocyte-to-TAM transition. revealed differentiate first into...
Eosinophils are a group of granulocytes well known for their capacity to protect the host from parasites and regulate immune function. Diverse biological roles eosinophils have been increasingly identified, but developmental pattern regulation eosinophil lineage remain largely unknown. Herein, we utilize zebrafish model analyze eosinophilic cell differentiation, distribution, regulation. By identifying eslec as an lineage-specific marker, establish Tg(eslec:eGFP) reporter line, which...
Abstract Uniform Manifold Approximation and Projection (UMAP) is a recently-published non-linear dimensionality reduction technique. Another such algorithm, t-SNE, has been the default method for task in past years. Herein we comment on usefulness of UMAP high-dimensional cytometry single-cell RNA sequencing, notably highlighting faster runtime consistency, meaningful organization cell clusters preservation continuums compared to t-SNE.
Circulating Ly6C hi monocytes often undergo cellular death upon exhaustion of their antibacterial effector functions, which limits capacity for subsequent macrophage differentiation. This shrouds the understanding on how host replaces tissue-resident niche effectively during bacterial invasion to avert infection morbidity. Here, we show that proliferating transitional premonocytes (TpMos), an immediate precursor mature (MatMos), were mobilized into periphery in response acute and sepsis....
Neutrophils are critical mediators during the early stages of innate inflammation in response to bacterial or fungal infections. A human hematopoietic system reconstituted humanized mice aids study hematology and immunology. However, poor development neutrophils is a well-known limitation mice. Here, we generate granulocyte colony-stimulating factor (hG-CSF) knockin (KI) NOD/Shi-scid-IL2rgnull (NOG) mouse which hG-CSF systemically expressed while G-CSF receptor disrupted. These high numbers...
Image cytometry is the process of converting image data to flow cytometry-style plots, and it usually requires computer-aided surface creation extract out statistics for cells or structures. One way dealing with structures stained multiple markers in three-dimensional images, carrying rounds channel co-localization masking before creation, which cumbersome laborious. We propose application hue-saturation-brightness color space streamline this process, produces complete surfaces, allows user...
Abstract SARS-CoV-2 is the novel coronavirus responsible for current COVID-19 pandemic. Severe complications are observed only in a small proportion of infected patients but cellular mechanisms underlying this progression still unknown. Comprehensive flow cytometry whole blood samples from 54 revealed dramatic increase number immature neutrophils. This strongly correlated with disease severity and was associated elevated IL-6 IP-10 levels, two key players cytokine storm. The most pronounced...