A5014621950- Gene expression and cancer classification
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Research and Splicing
- MicroRNA in disease regulation
- Bioinformatics and Genomic Networks
- Immune Cell Function and Interaction
- Congenital heart defects research
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Cardiac Fibrosis and Remodeling
- Genomics and Chromatin Dynamics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Angiogenesis and VEGF in Cancer
- Developmental Biology and Gene Regulation
- Immune Response and Inflammation
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
Spanish National Centre for Cardiovascular Research
2016-2025
Instituto de Salud Carlos III
2023-2025
Centro de Investigación en Red en Enfermedades Cardiovasculares
2023-2024
Centro de Investigación Biomédica en Red
2023-2024
Centro Nacional de Investigaciones Científicas
2019-2021
Hospital Universitario La Paz
2017
Royal Brompton & Harefield NHS Foundation Trust
2014
National Heart Centre Singapore
2014
Imperial College London
2014
Our Lady of Fatima Hospital
2014
The role of the adaptive immune response in controlling growth and recurrence human tumors has been controversial. We characterized tumor-infiltrating cells large cohorts colorectal cancers by gene expression profiling situ immunohistochemical staining. Collectively, immunological data (the type, density, location within tumor samples) were found to be a better predictor patient survival than histopathological methods currently used stage cancer. results validated two additional populations....
The role of tumor-infiltrating immune cells in the early metastatic invasion colorectal cancer is unknown.
Exosomes are released by most cells to the extracellular environment and involved in cell-to-cell communication. contain specific repertoires of mRNAs, microRNAs (miRNAs) other non-coding RNAs that can be functionally transferred recipient cells. However, mechanisms control loading RNA species into exosomes remain unknown. Here we describe sequence motifs present miRNAs their localization exosomes. The protein heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) specifically binds...
The immune synapse is an exquisitely evolved means of communication between T cells and antigen-presenting (APCs) during antigen recognition. Recent evidence points to the transfer RNA via exosomes as a novel mode intercellular communication. Here we show that T, B dendritic contain microRNA (miRNA) repertoires differ from those their parent cells. We investigate whether miRNAs are exchanged cognate interactions, demonstrate existence antigen-driven unidirectional cell APC, mediated by...
Abstract Summary: Despite the plethora of methods available for functional analysis omics data, obtaining comprehensive-yet detailed understanding results remains challenging. This is mainly due to lack publicly tools visualization this type information. Here we present an R package called GOplot, based on ggplot2, enhanced graphical representation. Our takes output any general enrichment and generates plots at different levels detail: from a overview identify most enriched categories (bar...
Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for development of novel treatment strategies, such as improved healing large bone defects. However, their low frequency in marrow necessitate ex vivo expansion further clinical application. In this study we asked if MSC are developing an aberrant or unwanted way during long-term cultivation and artificial conditions exert any influence on cell maintenance. To address question first...
Mesenchymal stem cells (MSCs) and osteolineage contribute to the hematopoietic cell (HSC) niche in bone marrow of long bones. However, their developmental relationships remain unclear. In this study, we demonstrate that different MSC populations developing bones have distinct functions. Proliferative mesoderm-derived nestin− MSCs participate fetal skeletogenesis lose activity soon after birth. contrast, quiescent neural crest-derived nestin+ preserve activity, but do not generate...
RNA-binding proteins (RBPs) play a crucial role in key cellular processes, including RNA transport, splicing, polyadenylation and stability. Understanding the interaction between RBPs is to improve our knowledge of processing, localization regulation global manner. Despite advances recent years, unified non-redundant resource that includes information on experimentally validated motifs, integrated tools exploit this lacking. Here, we developed database named ATtRACT (available at...
Abstract Aims Epigenetic age is emerging as a personalized and accurate predictor of biological age. The aim this article to assess the association subclinical atherosclerosis with accelerated epigenetic investigate underlying mechanisms mediating association. Methods results Whole blood methylomics, transcriptomics, plasma proteomics were obtained for 391 participants Progression Early Subclinical Atherosclerosis study. was calculated from methylomics data each participant. Its divergence...
Clonal hematopoiesis, a condition in which acquired somatic mutations hematopoietic stem cells lead to the outgrowth of mutant clone, is associated with higher risk hematological cancer and growing list nonhematological disorders, most notably atherosclerosis cardiovascular disease. However, whether accelerated cause or consequence clonal hematopoiesis remains matter debate. Some studies support direct contribution certain hematopoiesis-related via exacerbation inflammatory responses,...
Impairment of the intestinal barrier allows systemic translocation commensal bacteria, inducing a proinflammatory state in host. Here, we investigated innate immune responses following increased gut permeability upon administration dextran sulfate sodium (DSS) mice. We found that Enterococcus faecalis translocated to bone marrow DSS treatment and induced trained immunity (TI) hallmarks bone-marrow-derived mouse macrophages human monocytes. or heat-killed E. reprogrammed progenitors (BMPs),...
CARMAweb (Comprehensive R-based Microarray Analysis web service) is a application designed for the analysis of microarray data. performs data preprocessing (background correction, quality control and normalization), detection differentially expressed genes, cluster analysis, dimension reduction visualization, classification, Gene Ontology-term analysis. This accepts raw from variety imaging software tools most widely used platforms: Affymetrix GeneChips, spotted two-color microarrays Applied...
Highlights•Meis and Prep preferentially select different DNA-binding sequences in vivo•Prep shows a preference for promoters Meis promoter-remote enhancer regions•Meis associates much more than with embryonic patterning Hox target selection•TALE protein binding defines two global regulatory domains clustersSummaryThe interactions of Meis, Prep, Pbx1 TALE homeoproteins proteins are essential development disease. Although behave similarly vitro, their vivo activities remain largely unexplored....
microRNAs are master regulators of gene expression with essential roles in virtually all biological processes. miR-217 has been associated aging and cellular senescence, but its role vascular disease is not understood. Approach Results: We have used an inducible endothelium-specific knock-in mouse model to address the function atherosclerosis. reduced NO production promoted endothelial dysfunction, increased blood pressure, exacerbated atherosclerosis proatherogenic apoE-/- mice. Moreover,...
The metabolic injury caused by protein glycation, monitored as the level of glycated hemoglobin (HbA1c), is not represented in most risk scores (i.e., Systematic Coronary Risk Estimation or atherosclerotic cardiovascular disease scale). purpose this study was to assess association between HbA1c and extent subclinical atherosclerosis (SA) better identify individuals at higher extensive SA using on top key factors (CVRFs). A cohort 3,973 middle-aged from PESA (Progression Early Subclinical...
Atherosclerosis progression predicts cardiovascular events; however, of multiterritorial subclinical atherosclerosis is incompletely understood. This study sought to short-term using different noninvasive imaging techniques and their relationship with risk. The included 3,514 PESA (Progression Early Subclinical Atherosclerosis) participants (45.7 ± 4.2 years age; 63% men). Participants underwent 2-dimensional vascular ultrasound (2DVUS) abdominal aorta, carotid, iliac, femoral territories...
Abstract Aims Clinical guidelines recommend early intravenous β-blockers during ongoing myocardial infarction; however, it is unknown whether all exert a similar cardioprotective effect. We experimentally compared three clinically approved β-blockers. Methods and results Mice undergoing 45 min/24 h ischaemia–reperfusion (I/R) received vehicle, metoprolol, atenolol, or propranolol at min 35. The effect on neutrophil infiltration was tested in models of exacerbated inflammation. Neutrophil...
Imaging of subclinical atherosclerosis improves cardiovascular risk prediction on top traditional factors. However, imaging is not universally available. This work aims to identify circulating proteins that could predict atherosclerosis.Hypothesis-free proteomics was used analyze plasma from 444 subjects PESA cohort study (222 with extensive imaging, and 222 matched controls) at two timepoints (three years apart) for discovery, 350 AWHS (175 175 external validation. A selected three-protein...
Abstract Here we explored the role of interleukin-1β (IL-1β) repressor cytokine, IL-1 receptor antagonist (IL-1rn), in both healthy and abnormal hematopoiesis. Low IL-1RN is frequent acute myeloid leukemia (AML) patients represents a prognostic marker reduced survival. Treatments with IL-1β monoclonal antibody canakinumab reduce expansion leukemic cells, including CD34 + progenitors, AML xenografts. In vivo deletion IL-1rn induces hematopoietic stem cell (HSC) differentiation into lineage...
While generation of high-throughput expression data is becoming routine, the fast, easy, and systematic presentation analysis these in a biological context still an obstacle. To address this need, we have developed PathwayExplorer, which maps profiles genes or proteins simultaneously onto major, currently available regulatory, metabolic cellular pathways from KEGG, BioCarta GenMAPP. PathwayExplorer platform-independent web server application with optional standalone Java using SOAP (simple...