A5075098122- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Renal cell carcinoma treatment
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Renal and related cancers
- Complement system in diseases
- Immune cells in cancer
- Ferroptosis and cancer prognosis
- Bladder and Urothelial Cancer Treatments
- Blood groups and transfusion
- Cancer Genomics and Diagnostics
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Epigenetics and DNA Methylation
- Cell Adhesion Molecules Research
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Cancer Research and Treatments
- Protein purification and stability
- Renal Diseases and Glomerulopathies
- Colorectal Cancer Treatments and Studies
Centre de Recherche des Cordeliers
2016-2025
Inserm
2016-2025
Sorbonne Université
2016-2025
Université Paris Cité
2016-2025
La Ligue Contre le Cancer
2013-2024
Assistance Publique – Hôpitaux de Paris
2007-2023
Délégation Paris 5
2012-2023
Sorbonne Paris Cité
2011-2023
Hôpital Européen Georges-Pompidou
2007-2023
Fontbonne University
2023
The role of the adaptive immune response in controlling growth and recurrence human tumors has been controversial. We characterized tumor-infiltrating cells large cohorts colorectal cancers by gene expression profiling situ immunohistochemical staining. Collectively, immunological data (the type, density, location within tumor samples) were found to be a better predictor patient survival than histopathological methods currently used stage cancer. results validated two additional populations....
Abstract Summary: We have developed ClueGO, an easy to use Cytoscape plug-in that strongly improves biological interpretation of large lists genes. ClueGO integrates Gene Ontology (GO) terms as well KEGG/BioCarta pathways and creates a functionally organized GO/pathway term network. It can analyze one or compare two genes comprehensively visualizes grouped terms. A one-click update option allows automatically download the most recent GO/KEGG release at any time. provides intuitive...
We introduce the Microenvironment Cell Populations-counter (MCP-counter) method, which allows robust quantification of absolute abundance eight immune and two stromal cell populations in heterogeneous tissues from transcriptomic data. present vitro mRNA mixture ex vivo immunohistochemical data that quantitatively support validity our method's estimates. Additionally, we demonstrate MCP-counter overcomes several limitations or weaknesses previously proposed computational approaches. is...
The role of tumor-infiltrating immune cells in the early metastatic invasion colorectal cancer is unknown.
Soft-tissue sarcomas represent a heterogeneous group of cancer, with more than 50 histological subtypes1,2. The clinical presentation patients different subtypes is often atypical, and responses to therapies such as immune checkpoint blockade vary widely3,4. To explain this variability, here we study gene expression profiles in 608 tumours across soft-tissue sarcoma. We establish an immune-based classification on the basis composition tumour microenvironment identify five distinct...
The tumor microenvironment includes a complex network of immune T-cell subpopulations. In this study, we systematically analyzed the balance between cytotoxic T cells and different subsets helper in human colorectal cancers correlated their impact on disease-free survival. A panel related genes were 125 frozen specimens. Infiltrating cells, Treg, Th1, Th17 also quantified center invasive margin tumors. By hierarchical clustering correlation matrix identified functional clusters associated...
Purpose The prognosis of patients with colorectal cancer has sometimes proved uncertain; thus, the prognostic significance immune criteria was compared that tumor extension using American Joint Committee on Cancer/International Union Against Cancer–TNM (AJCC/UICC-TNM) staging system. Patients and Methods We studied intratumoral infiltrates in center invasive margin 599 specimens stage I to IV cancers from two independent cohorts. analyzed these findings relation degree frequency recurrence....
Many patients who present with early-stage colorectal cancer (International Union Against Cancer TNM stages I and II) are nevertheless at high risk of relapse. We hypothesized that intratumoral immune reaction could influence their prognosis.The was investigated in 29 tumors by large-scale real-time polymerase chain reaction. Cytotoxic (CD8) memory (CD45RO) T cells were quantified immunohistochemical analyses tissue microarrays from the center (CT) invasive margin (IM) 602 two independent...
Purpose It has been established that the immune system plays an important role in tumor rejection. There is also compelling evidence responses can develop independently of secondary lymphoid organs tertiary structures. We studied presence and correlation structures with clinical outcome non–small-cell lung cancer (NSCLC), as prognostic value these patients had not yet established. Patients Methods This retrospective study was performed by immunohistochemistry on paraffin-embedded tissue...
The composition and density of immune cells in the tumor microenvironment (TME) profoundly influence progression success anti-cancer therapies. Flow cytometry, immunohistochemistry staining or single-cell sequencing are often unavailable such that we rely on computational methods to estimate immune-cell from bulk RNA-sequencing (RNA-seq) data. Various have been proposed recently, yet their capabilities limitations not evaluated systematically. A general guideline leading research community...
It is now well established that immune responses can take place outside of primary and secondary lymphoid organs. We previously described the presence tertiary structures (TLS) in patients with non-small cell lung cancer (NSCLC) characterized by clusters mature dendritic cells (DCs) T surrounded B-cell follicles. demonstrated density these DCs was associated favorable clinical outcome.To study role follicular B TLS potential link a local humoral response NSCLC.The cellular composition...
Head and neck cancers positive for human papillomavirus (HPV) have a more favorable clinical outcome than HPV-negative cancers, but it is unknown why this the case. We hypothesized that prognosis was affected by intrinsic features of HPV-infected tumor cells or differences in host immune response. In study, we focused on comparison regulatory Foxp3(+) T programmed death-1 (PD-1)(+) microenvironment tumors were negative HPV, two groups matched various biologic parameters. HPV-positive head...
Abstract Purpose: CD4+ T cells play a central role in initiating and maintaining anticancer immune responses. However, regulatory CD4+CD25+ which express Foxp3 have also been shown to inhibit antitumor effector cells. In view of these heterogeneous T-cell populations, this study was designed determine the prognostic value various tumor-infiltrating populations head neck squamous cell carcinoma. Experimental Design: Eighty-four newly diagnosed untreated patients with histologically proven...
Tumor-infiltrating T cells, particularly CD45RO(+)CD8(+) memory confer a positive prognostic value in human cancers. However, the mechanisms that promote protective T-cell response tumor microenvironment remain unclear. In chronic inflammatory settings such as microenvironment, lymphoid neogenesis can occur to create local lymph node-like structures known tertiary (TLS). These exacerbate immune response, TLS formation tumors may help an efficacious contexture. role of has yet be investigated...
Several studies have demonstrated genetic predisposition in non-shigatoxin-associated hemolytic uremic syndrome (HUS), involving regulatory proteins of the complement alternative pathway: Factor H (FH) and membrane co-factor protein (CD46). Regarding observations thrombotic thrombocytopenic purpura patients, whom a von Willebrand factor protease (ADAMST-13) deficiency may be inherited or acquired secondary to IgG antibodies, it was speculated that HUS might occur context an autoimmune...
The tumor microenvironment is formed by many distinct and interacting cell populations, its composition may predict patients' prognosis response to therapies. Colorectal cancer a heterogeneous disease in which immune classifications four consensus molecular subgroups (CMS) have been described. Our aim was integrate the of with classification colorectal cancer.We retrospectively analyzed functional orientation immune, fibroblastic, angiogenic 1,388 tumors from three independent cohorts using...
B lymphocytes and macrophages express closely related immunoglobulin G (IgG) Fc receptors (Fc gamma RII) that differ only in the structures of their cytoplasmic domains. Because cell type-specific alternative messenger RNA splicing, B-cell RII contains an insertion 47 amino acids participates determining receptor function these cells. Transfection RII-negative line with complementary DNA's encoding two splice products various mutants indicated was responsible for preventing both RII-mediated...