A5042627121- Cancer Immunotherapy and Biomarkers
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Renal cell carcinoma treatment
- Occupational and environmental lung diseases
- Adrenal and Paraganglionic Tumors
- Ferroptosis and cancer prognosis
- Epigenetics and DNA Methylation
- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Cancer Research and Treatments
- Renal and related cancers
- Lymphoma Diagnosis and Treatment
- Bladder and Urothelial Cancer Treatments
- Glioma Diagnosis and Treatment
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Medical Imaging and Pathology Studies
- Cancer Cells and Metastasis
- Colorectal and Anal Carcinomas
- Urinary and Genital Oncology Studies
Centre de Recherche des Cordeliers
2018-2025
Inserm
2009-2025
Sorbonne Université
2010-2025
Université Paris Cité
2009-2025
Centre National de la Recherche Scientifique
2010-2025
Université de Pau et des Pays de l'Adour
2025
La Ligue Contre le Cancer
2015-2024
Assistance Publique – Hôpitaux de Paris
2009-2023
Université Grenoble Alpes
2023
Centre Hospitalier Universitaire de Grenoble
2023
We introduce the Microenvironment Cell Populations-counter (MCP-counter) method, which allows robust quantification of absolute abundance eight immune and two stromal cell populations in heterogeneous tissues from transcriptomic data. present vitro mRNA mixture ex vivo immunohistochemical data that quantitatively support validity our method's estimates. Additionally, we demonstrate MCP-counter overcomes several limitations or weaknesses previously proposed computational approaches. is...
Soft-tissue sarcomas represent a heterogeneous group of cancer, with more than 50 histological subtypes1,2. The clinical presentation patients different subtypes is often atypical, and responses to therapies such as immune checkpoint blockade vary widely3,4. To explain this variability, here we study gene expression profiles in 608 tumours across soft-tissue sarcoma. We establish an immune-based classification on the basis composition tumour microenvironment identify five distinct...
Background Colon cancer (CC) pathological staging fails to accurately predict recurrence, and date, no gene expression signature has proven reliable for prognosis stratification in clinical practice, perhaps because CC is a heterogeneous disease. The aim of this study was establish comprehensive molecular classification based on mRNA profile analyses. Methods Findings Fresh-frozen primary tumor samples from large multicenter cohort 750 patients with stage I IV who underwent surgery between...
Hepatocellular carcinomas (HCCs) are a heterogeneous group of tumors that differ in risk factors and genetic alterations. We further investigated transcriptome-genotype-phenotype correlations HCC. Global transcriptome analyses were performed on 57 HCCs 3 hepatocellular adenomas validated by quantitative RT-PCR using 63 additional HCCs. determined loss heterozygosity, gene mutations, promoter methylation CDH1 CDKN2A , HBV DNA copy number for each tumor. Unsupervised analysis identified 6...
The tumor microenvironment is formed by many distinct and interacting cell populations, its composition may predict patients' prognosis response to therapies. Colorectal cancer a heterogeneous disease in which immune classifications four consensus molecular subgroups (CMS) have been described. Our aim was integrate the of with classification colorectal cancer.We retrospectively analyzed functional orientation immune, fibroblastic, angiogenic 1,388 tumors from three independent cohorts using...
Large-scale detection of ectopic gene activations reveals a source potential cancer biomarkers and correlates with aggressive metastasis-prone lung tumors.
Significance The exclusion of immune cells from the tumor microenvironment has been associated with poor prognosis in majority cancers. We report that when considering 21 solid cancer types, cell is widely presence a stem cell-like phenotype tumors (“stemness”). Stemness positively correlates higher intratumoral heterogeneity, possibly by protecting antigenic clones elimination system. activation stemness program appears to limit antitumor responses via cell-intrinsic silencing endogenous...
Purpose Adrenocortical tumors, especially cancers, remain challenging both for their diagnosis and prognosis assessment. The aim of this article is to identify molecular predictors malignancy survival. Patients Methods One hundred fifty-three unilateral adrenocortical tumors were studied by microarray (n = 92) or reverse transcription quantitative polymerase chain reaction 148). A two-gene predictor was built using the disease-free survival as end point in a training cohort 47), then...
Muscle-invasive bladder carcinoma (MIBC) constitutes a heterogeneous group of tumors with poor outcome. Molecular stratification MIBC may identify clinically relevant tumor subgroups and help to provide effective targeted therapies. From seven series large-scale transcriptomic data (383 tumors), we identified an subgroup accounting for 23.5% MIBC, associated shorter survival displaying basal-like phenotype, as shown by the expression epithelial basal cell markers. Basal-like presented...
Pulmonary large-cell neuroendocrine carcinomas (LCNECs) have similarities with other lung cancers, but their precise relationship has remained unclear. Here we perform a comprehensive genomic (n = 60) and transcriptomic 69) analysis of 75 LCNECs identify two molecular subgroups: "type I LCNECs" bi-allelic TP53 STK11/KEAP1 alterations (37%), II enriched for inactivation RB1 (42%). Despite sharing adenocarcinomas squamous cell carcinomas, no transcriptional was found; instead form distinct...
Pheochromocytomas and paragangliomas are neuroendocrine tumors that occur in the context of inherited cancer syndromes ∼30% cases linked to germline mutations VHL, RET, NF1, SDHA, SDHB, SDHC, SDHD, SDHAF2 TMEM127 genes. Although genome-wide expression studies have revealed some mechanisms likely be involved pheochromocytoma/paraganglioma tumorigenesis, complete molecular distinction all subtypes hereditary has not been solved genetic events generation sporadic unknown. With these purposes...
Abstract Purpose: Selecting patients with metastatic clear-cell renal cell carcinoma (m-ccRCC) who might benefit from treatment targeted tyrosine kinase inhibitors (TKI) is a challenge. Our aim was to identify molecular markers associated outcome in m-ccRCC treated sunitinib. Experimental Design: We performed global transcriptome analyses on 53 primary resected ccRCC tumors developed disease and were first-line also determined chromosome copy-number aberrations, methylation status, gene...
The current histoclinical breast cancer classification is simple but imprecise. Several molecular classifications of cancers based on expression profiling have been proposed as alternatives. However, their reliability and clinical utility repeatedly questioned, notably because most them were derived from relatively small initial patient populations. We analyzed the transcriptomes 537 tumors using three unsupervised methods. A core subset 355 was assigned to six clusters by all These...