A5033716671- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Breast Cancer Treatment Studies
- Acute Myeloid Leukemia Research
- Gene expression and cancer classification
- Epigenetics and DNA Methylation
- Genomic variations and chromosomal abnormalities
- HER2/EGFR in Cancer Research
- Fibroblast Growth Factor Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- BRCA gene mutations in cancer
- Chronic Myeloid Leukemia Treatments
- Bioinformatics and Genomic Networks
- Microtubule and mitosis dynamics
- Protein Degradation and Inhibitors
- Genomics and Chromatin Dynamics
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- Histone Deacetylase Inhibitors Research
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Pancreatic and Hepatic Oncology Research
- Chronic Lymphocytic Leukemia Research
- Molecular Biology Techniques and Applications
Centre National de la Recherche Scientifique
2015-2024
Aix-Marseille Université
2015-2024
Institut Paoli-Calmettes
2015-2024
Inserm
2015-2024
Centre de Recherche en Cancérologie de Marseille
2015-2024
La Ligue Contre le Cancer
2023-2024
Institut Pprime
2011-2023
Molecular Oncology (United States)
1996-2023
Cancer Institute (WIA)
2005-2023
Cancer Research Center
2007-2023
Tumors may be initiated and maintained by a cellular subcomponent that displays stem cell properties. We have used the expression of aldehyde dehydrogenase as assessed ALDEFLUOR assay to isolate characterize cancer (CSC) populations in 33 lines derived from normal malignant mammary tissue. Twenty-three contained an ALDEFLUOR-positive population displayed properties vitro NOD/SCID xenografts. Gene profiling identified 413-gene CSC profile included genes known play role function, well such...
Previous studies have suggested that breast cancer stem cells (BCSCs) mediate metastasis, are resistant to radiation and chemotherapy, contribute relapse. Although several BCSC markers been described, it is unclear whether these identify the same or independent BCSCs. Here, we show BCSCs exist in distinct mesenchymal-like (epithelial-mesenchymal transition [EMT]) epithelial-like (mesenchymal-epithelial [MET]) states. Mesenchymal-like characterized as CD24−CD44+ primarily quiescent localized...
To examine the role of cancer stem cells (CSC) in mediating metastasis inflammatory breast (IBC) and association these with patient outcome this aggressive type cancer.CSCs were isolated from SUM149 MARY-X, an IBC cell line primary xenograft, by virtue increased aldehyde dehydrogenase (ALDH) activity as assessed ALDEFLUOR assay. Invasion CSC populations vitro mouse xenograft assays. Expression ALDH1 was determined on a retrospective series 109 patients correlated histoclinical data. All...
Recent evidence suggests that breast cancer and other solid tumors possess a rare population of cells capable extensive self-renewal contribute to metastasis treatment resistance. We report here the development strategy target these stem (CSCs) through blockade IL-8 receptor CXCR1. CXCR1 using either CXCR1-specific blocking antibody or repertaxin, small-molecule inhibitor, selectively depleted CSC in 2 human cell lines vitro. Furthermore, this was followed by induction massive apoptosis bulk...
Transcriptome sequencing improves the diagnostic rate for Mendelian disease in patients whom genetic analysis has not returned a diagnosis.
Worldwide, hundreds of thousands humans have had their genomes or exomes sequenced, and access to the resulting data sets can provide valuable information for variant interpretation understanding gene function. Here, we present a lightweight, flexible browser framework display large population datasets genetic variation. We demonstrate its use exome sequence from 60 706 individuals in Exome Aggregation Consortium (ExAC). The ExAC provides gene- transcript-centric displays variation, critical...
NK cells are a major component of the antitumor immune response and involved in controlling tumor progression metastases animal models. Here, we show that dysfunction these accompanies human breast progression. We characterized peripheral blood (p-NK) malignant mammary tumor-infiltrating (Ti-NK) from patients with noninvasive invasive cancers. isolated healthy donors normal tissue were used as controls. With disease progression, found expression activating cell receptors (such NKp30, NKG2D,...
The myelodysplastic syndromes (MDSs) are a heterogeneous group of clonal haematological diseases characterized by ineffective haematopoiesis and predisposition to acute myeloid leukaemia (AML). pathophysiology MDSs remains unclear. A definition the molecular biology may lead better classification, new prognosis indicators treatments. We studied series 40 MDS/AML samples high-density array-comparative genome hybridization (aCGH). displayed few alterations that can point candidate genes, which...
Purpose Several prognostic scoring systems have been proposed for chronic myelomonocytic leukemia (CMML), a disease in which some gene mutations—including ASXL1—have associated with poor prognosis univariable analyses. We developed and validated score overall survival (OS) based on mutational status standard clinical variables. Patients Methods genotyped ASXL1 up to 18 other genes including epigenetic (TET2, EZH2, IDH1, IDH2, DNMT3A), splicing (SF3B1, SRSF2, ZRSF2, U2AF1), transcription...
// Renaud Sabatier 1,2,3 , Pascal Finetti 1 Emilie Mamessier José Adelaide Max Chaffanet Hamid Raza Ali 4,5 Patrice Viens Carlos Caldas 5 Daniel Birnbaum and François Bertucci Département d’Oncologie Moléculaire, “Equipe labellisée Ligue Contre le Cancer”, Centre de Recherche en Cancérologie Marseille (CRCM), Institut Paoli-Calmettes, INSERM UMR1068, CNRS UMR725, Marseille, France 2 Médicale, CRCM, 3 Faculté...
The basal molecular subtype of breast cancer (BC) is defined by the mRNA expression pattern an intrinsic approximately 500-gene set. It most homogeneous in transcriptional terms, and one aggressive prognostic terms. Clinical trials testing new systemic therapeutic strategies have been launched BCs. Although no proof evidence has yet reported, tumors are currently assimilated to selected as triple-negative (TN) BCs these because their frequent immunohistochemical (IHC) negativity for hormone...
The current histoclinical breast cancer classification is simple but imprecise. Several molecular classifications of cancers based on expression profiling have been proposed as alternatives. However, their reliability and clinical utility repeatedly questioned, notably because most them were derived from relatively small initial patient populations. We analyzed the transcriptomes 537 tumors using three unsupervised methods. A core subset 355 was assigned to six clusters by all These...
Monoclonal antibodies are among the most promising therapeutic agents for treating cancer. Therapeutic cancer bind to tumor cells, turning them into targets immune-mediated destruction. We show here that this antibody-mediated killing of cells is limited by a mechanism involving interaction between cell-expressed CD47 and inhibitory receptor signal regulatory protein-α (SIRPα) on myeloid cells. Mice lack SIRPα cytoplasmic tail, hence its signaling, display increased elimination melanoma in...