A5043678912- Bladder and Urothelial Cancer Treatments
- Bioinformatics and Genomic Networks
- Gene expression and cancer classification
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Genomic variations and chromosomal abnormalities
- Urinary and Genital Oncology Studies
- Cancer, Hypoxia, and Metabolism
- Fibroblast Growth Factor Research
- Ubiquitin and proteasome pathways
- Proteoglycans and glycosaminoglycans research
- Phagocytosis and Immune Regulation
- Protein Tyrosine Phosphatases
- Renal cell carcinoma treatment
- Cell Adhesion Molecules Research
- Cancer-related Molecular Pathways
- Genomics and Chromatin Dynamics
- Cancer Research and Treatments
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Peroxisome Proliferator-Activated Receptors
- Ferroptosis and cancer prognosis
- Apelin-related biomedical research
Institut Curie
2014-2024
Centre National de la Recherche Scientifique
2014-2024
Université Paris Sciences et Lettres
2017-2024
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2010-2023
Université Paris Cité
2002-2023
Sorbonne Université
2018-2023
Biologie cellulaire et Cancer
2019-2023
La Ligue Contre le Cancer
2021-2023
Laboratoire de Biologie et Modélisation de la Cellule
2022
Centre de Gestion Scientifique
2013
Muscle-invasive bladder carcinoma (MIBC) constitutes a heterogeneous group of tumors with poor outcome. Molecular stratification MIBC may identify clinically relevant tumor subgroups and help to provide effective targeted therapies. From seven series large-scale transcriptomic data (383 tumors), we identified an subgroup accounting for 23.5% MIBC, associated shorter survival displaying basal-like phenotype, as shown by the expression epithelial basal cell markers. Basal-like presented...
Extracting relevant information from large-scale data offers unprecedented opportunities in cancerology. We applied independent component analysis (ICA) to bladder cancer transcriptome sets and interpreted the components using gene enrichment tumor-associated molecular, clinicopathological, processing information. identified associated with biological processes of tumor cells or microenvironment, other revealed technical biases. Applying ICA nine types cancer-shared bladder-cancer-specific...
The Mitogen-Activated Protein Kinase (MAPK) network consists of tightly interconnected signalling pathways involved in diverse cellular processes, such as cell cycle, survival, apoptosis and differentiation. Although several studies reported the involvement these cascades cancer deregulations, precise mechanisms underlying their influence on balance between proliferation death (cell fate decision) pathological circumstances remain elusive. Based an extensive analysis published data, we have...
Abstract Retinoblastoma is the most frequent intraocular malignancy in children, originating from a maturing cone precursor developing retina. Little known on molecular basis underlying biological and clinical behavior of this cancer. Here, using multi-omics data, we demonstrate existence two retinoblastoma subtypes. Subtype 1, earlier onset, includes heritable forms. It harbors few genetic alterations other than initiating RB1 inactivation corresponds to differentiated tumors expressing...
Germinal activating mutations of FGFR3 are responsible for several forms dwarfism due to the inhibitory effect on bone growth. Surprisingly, identical somatic have been found at level in tumours: high frequency benign epithelial tumours (seborrheic keratosis, urothelial papilloma) and low-grade, low-stage carcinomas, a lower other types carcinoma, cervix haematological cancer, multiple myeloma. exists as two isoforms, FGFR3b FGFR3c, differs ligand specificity tissue expression. is main form...
Abstract The 8p11-12 chromosome region is one of the regions most frequently amplified in breast carcinoma (10–15% cases). Several genes within this have been identified as candidate oncogenes, they are both and overexpressed. However, very few studies explored role these cell transformation, with aim identifying valuable therapeutic targets. An analysis comparative genomic hybridization array expression profiling data for a series 152 ductal carcinomas 21 lines five (LSM1, BAG4, DDHD2,...
Research Article20 February 2018Open Access Source DataTransparent process An FGFR3/MYC positive feedback loop provides new opportunities for targeted therapies in bladder cancers Mélanie Mahe Institut Curie, CNRS, UMR144, Equipe Labellisée Ligue contre le Cancer, PSL University, Paris, France Sorbonne Universités, UPMC Université Paris 06, Search more papers by this author Florent Dufour Hélène Neyret-Kahn Aura Moreno-Vega Claire Beraud UROLEAD SAS, School of Medicine, Strasbourg, Mingjun...
Abstract Background APOBEC-driven mutagenesis and functional positive selection of mutated genes may synergistically drive the higher frequency some hotspot driver mutations compared to other within same gene, as we reported for FGFR3 S249C. Only a few APOBEC-associated have been identified in bladder cancer (BCa). Here, systematically looked characterised hotspots BCa. Methods We analysed 602 published exome-sequenced BCas, part which gene expression data were also available. by...
Epigenetic silencing can extend to whole chromosomal regions in cancer. There have been few genome-wide studies exploring its involvement tumorigenesis. We searched for affected by epigenetic cancer using Affymetrix microarrays and real-time quantitative polymerase chain reaction analyze RNA from 57 bladder tumors compared with normal urothelium. was verified gene re-expression following treatment of cell lines 5-aza-deoxycytidine, a DNA demethylating agent, trichostatin A, histone...
The upregulation of PPARγ/RXRα transcriptional activity has emerged as a key event in luminal bladder tumors. It renders tumor cell growth PPARγ-dependent and modulates the microenvironment to favor escape from immuno-surveillance. activation pathway been linked PPARG gains/amplifications resulting PPARγ overexpression recurrent activating point mutations RXRα. Here, we report that also activate pathway, conferring PPARγ-dependency supporting crucial role cancer. These are found throughout...
Abstract Interleukin-7 receptor α (encoded by IL7R ) is essential for lymphoid development. Whether acute lymphoblastic leukemia (ALL)-related gain-of-function mutations can trigger leukemogenesis remains unclear. Here, we demonstrate that lymphoid-restricted mutant , expressed at physiological levels in conditional knock-in mice, establishes a pre-leukemic stage which B-cell precursors display self-renewal ability, initiating resembling PAX5 P80R or Ph-like human B-ALL. Full transformation...
Molecular understanding of muscle-invasive (MIBC) and non–muscle-invasive (NMIBC) bladder cancer is currently based primarily on transcriptomic genomic analyses. To conduct proteogenomic analyses to gain insights into (BC) heterogeneity identify underlying processes specific tumor subgroups therapeutic outcomes. Proteomic data were obtained for 40 MIBC 23 NMIBC cases which already available. Four BC-derived cell lines harboring FGFR3 alterations tested with interventions. Recombinant...
Abstract Completion of the working draft human genome has made it possible to analyze expression genes according their position on chromosomes. Here, we used a transcriptome data analysis approach involving for each gene calculation correlation between its profile and those neighbors. We U133 Affymetrix set series 130 invasive ductal breast carcinomas construct chromosomal maps (transcriptome map). This highlighted nonrandom clusters along with correlated in tumors. Some identified by this...
RNA interference has boosted the field of functional genomics, by making it possible to carry out 'loss-of-function' screens in cultured cells. Here, we performed a small interfering screening, three breast cancer cell lines, for 101 candidate driver genes overexpressed amplified tumors and belonging eight amplicons on chromosomes 8q 17q, investigating their role survival/proliferation. This screening identified that were amplified, critical tumor proliferation or survival. They included...
Milk Fat Globule – EGF factor VIII (MFGE8), also called lactadherin, is a secreted protein, which binds extracellularly to phosphatidylserine and αvβ3 αvβ5 integrins. On human mouse cells expressing these integrins, such as endothelial cells, phagocytes some tumors, MFGE8/lactadherin has been shown promote survival, epithelial mesenchymal transition phagocytosis. A protumoral function of MFGE8 consequently documented for few types cancers, including melanoma, subtype breast bladder...
The aggressive basal/squamous (Ba/Sq) bladder cancer (BLCA) subtype is often diagnosed at the muscle-invasive stage and can progress to sarcomatoid variant. Identification of molecular changes occurring during progression from non-muscle-invasive BLCA (NMIBC) Ba/Sq (MIBC) thus challenging in human disease. We used N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN) mouse model MIBC study longitudinally leading phenotype variant using IHC microdissection followed by RNA-seq all stages progression. A...
Abstract Muscle-invasive bladder cancer (BLCA) is an aggressive disease. Consensus BLCA transcriptomic subtypes have been proposed, with two major Luminal and Basal subgroups, presenting distinct molecular clinical characteristics. However, how these are regulated remains unclear. We hypothesized that epigenetic activation of super-enhancers could drive the transcriptional programs subtypes. Through integrated RNA-sequencing epigenomic profiling histone marks in primary tumours, cell lines,...
Muscle-invasive bladder cancer (MIBC) is an aggressive neoplasm with poor prognosis, lacking effective therapeutic targets. Oncogenic dependency on members of the TAM tyrosine kinase receptor family (TYRO3, AXL, MERTK) has been reported in several types, but their role never explored. expression was evaluated two series human tumours by gene (TCGA and CIT series), immunohistochemistry western blotting analyses (CIT series). The different receptors assessed loss-of-function experiments...