A5019975916- Bladder and Urothelial Cancer Treatments
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Urinary and Genital Oncology Studies
- Cancer Immunotherapy and Biomarkers
- Genetic factors in colorectal cancer
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Renal cell carcinoma treatment
- Ferroptosis and cancer prognosis
- Diagnosis and treatment of tuberculosis
- Esophageal Cancer Research and Treatment
- Circular RNAs in diseases
- Molecular Biology Techniques and Applications
- Cancer Diagnosis and Treatment
- Lung Cancer Diagnosis and Treatment
- RNA Research and Splicing
- Lung Cancer Treatments and Mutations
- Myasthenia Gravis and Thymoma
- Tuberculosis Research and Epidemiology
- Hematological disorders and diagnostics
- Prostate Cancer Treatment and Research
- Gene expression and cancer classification
- Pleural and Pulmonary Diseases
Aarhus University Hospital
2016-2025
Aarhus University
2006-2023
Vestergaard (Switzerland)
2013
Tampere University Hospital
2013
Tampere University
2013
Heinrich Heine University Düsseldorf
2013
Düsseldorf University Hospital
2013
PURPOSE Novel sensitive methods for early detection of relapse and monitoring therapeutic efficacy may have a huge impact on risk stratification, treatment, ultimately outcome patients with bladder cancer. We addressed the prognostic predictive ultra-deep sequencing cell-free DNA in before after cystectomy during chemotherapy. PATIENTS AND METHODS included 68 localized advanced Patient-specific somatic mutations, identified by whole-exome sequencing, were used to assess circulating tumor...
microRNAs (miRNA) are involved in cancer development and progression, acting as tumor suppressors or oncogenes. Here, we profiled the expression of 290 unique human miRNAs 11 normal 106 bladder samples using spotted locked nucleic acid-based oligonucleotide microarrays. We identified several differentially expressed between urothelium different disease stages. miR-145 was found to be most down-regulated compared with normal, miR-21 up-regulated cancer. Furthermore, that significantly...
Abstract Purpose: We investigated whether detection of ctDNA after resection colorectal cancer identifies the patients with highest risk relapse and, furthermore, longitudinal analysis allows early and informs about response to intervention. Experimental Design: In this cohort study, we used massively parallel sequencing identify somatic mutations these as markers detect minimal residual disease monitor changes in tumor burden during a 3-year follow-up period. Results: A total 45 371 plasma...
Abstract Exosomes are small secreted vesicles that can transfer their content to recipient cells. In cancer, exosome secretion has been implicated in tumor growth and metastatic spread. this study, we explored the possibility exosomal pathways might discard tumor-suppressor miRNA restricts progression. Secreted characterized from isogenic bladder carcinoma cell lines with differing potential were uncoupled binding target transcripts or AGO2–miRISC complex. cells, observed a relative increase...
Abstract The molecular landscape in non-muscle-invasive bladder cancer (NMIBC) is characterized by large biological heterogeneity with variable clinical outcomes. Here, we perform an integrative multi-omics analysis of patients diagnosed NMIBC ( n = 834). Transcriptomic identifies four classes (1, 2a, 2b and 3) reflecting tumor biology disease aggressiveness. Both transcriptome-based subtyping the level chromosomal instability provide independent prognostic value beyond established...
Overtreatment with cisplatin-based chemotherapy is a major issue in the management of muscle-invasive bladder cancer (MIBC), and currently none reported biomarkers for predicting response have been implemented clinic. Here we perform comprehensive multi-omics analysis (genomics, transcriptomics, epigenomics proteomics) 300 MIBC patients treated (neoadjuvant or first-line) to identify molecular changes associated treatment response. DNA-based associations converge on genomic instability...
Circulating tumor DNA (ctDNA) can be used for sensitive detection of minimal residual disease (MRD). However, the probability detecting ctDNA in settings low burden is limited by number mutations analyzed and plasma volume available. We a whole-genome sequencing (WGS) approach patients with urothelial carcinoma.
Epigenetic alterations are common and can now be addressed in a parallel fashion. We investigated the methylation bladder cancer with respect to location genome, consistency, variation metachronous tumors, impact on transcripts, chromosomal location, usefulness as urinary markers.A microarray assay was utilized analyze 56 samples. Independent validation conducted 63 samples by PCR-based method bisulfite sequencing. The levels 174 urine specimens were quantified. Transcript analyzed using...
Abstract Background microRNAs (miRNA) are short, endogenous transcripts that negatively regulate the expression of specific mRNA targets. miRNAs found both in tissues and body fluids such as plasma. A major perspective for use clinical setting is diagnostic plasma markers neoplasia. While abundant tissues, they often scarce For quantification miRNA it therefore importance to a platform with high sensitivity linear performance low concentration range. This motivated us evaluate three commonly...
In our study, whole-genome methylation arrays were applied to identify novel genes with tumor specific DNA of promoter CpG islands in pre-malignant and malignant colorectal lesions. Using a combination Illumina HumanMethylation27 beadchips, Methylation-Sensitive High Resolution Melting (MS-HRM) analysis, Exon (Affymetrix) the pattern ∼14,000 their transcript levels investigated six normal mucosas, adenomas 30 MSI MSS carcinomas. Sixty eight tumor-specific hypermethylation identified (p <...
Bladder cancer (or urothelial cell carcinoma [UCC]) is characterized by field disease (malignant alterations in surrounding mucosa) and frequent recurrences. Whole-genome, exome, transcriptome sequencing of 38 tumors, including four metachronous tumor pairs 20 superficial identified an APOBEC mutational signature one-third. This was biased toward the sense strand, correlated with mean expression level, clustered near breakpoints. A>G mutations were up to eight times more on strand (p<0.002)...
Adults with 45,X monosomy (Turner syndrome) reflect a surviving minority since more than 99% of fetuses die in utero. In adulthood is associated increased morbidity and mortality, although strikingly heterogeneous some individuals left untouched while others suffer from cardiovascular disease, autoimmune disease infertility. The present study investigates the leukocyte DNAmethylation profile by using 450K-Illumina Infinium assay RNA-expression compared karyotypically normal female male...
Genetic alterations identified in adjacent normal appearing tissue bladder cancer patients are indicative of a field disease. Here we assessed urothelium transformation and intra-tumour heterogeneity (ITH) four with cancer. Exome sequencing private acquired mutations lymph node metastasis local recurrences. Deep re-sequencing revealed presence at least three subclones two multifocal disease, while no demarcation was the unifocal Analysis showed low frequency Expression profiling...
Purpose Diagnostic and prognostic tools for prostate cancer (PC) are suboptimal, causing overtreatment of indolent PC risk delayed treatment aggressive PC. Here, we identify six novel candidate DNA methylation markers with promising diagnostic potential. Methods Microarray-based screening bisulfite sequencing 20 nonmalignant 29 tissue specimens were used to new hypermethylation potential was evaluated in 35 samples, 293 radical prostatectomy (RP) samples (cohort 1, training), 114 malignant...
Colorectal cancer (CRC) is one of the leading causes deaths in Western countries. A significant number CRC patients undergoing curatively intended surgery subsequently develop recurrence and die from disease. MicroRNAs (miRNAs) are aberrantly expressed cancers appear to have both diagnostic prognostic significance. In this study, we identified novel miRNAs associated with CRC, their possible mechanism action. TaqMan ® Human MicroRNA Array Set v2.0 was used profile expression 667 14 normal...
Greater knowledge concerning tumor heterogeneity and clonality is needed to determine the impact of targeted treatment in setting bladder cancer. In this study, we performed whole-exome, transcriptome, deep-focused sequencing metachronous tumors from 29 patients initially diagnosed with early-stage (14 nonprogressive disease 15 progressive disease). Tumors showed a higher variance intrapatient mutational spectrum frequency APOBEC-related mutations. Allele-specific expression was also these...
The functional status of immune cells in the tumor microenvironment and characteristics may explain bacillus Calmette-Guérin (BCG) failure high-risk non-muscle-invasive bladder cancer (NMIBC).To characterize molecular correlates post-BCG high-grade (HG) recurrence using multiomics analysis.Patients with BCG-treated NMIBC (n = 156) were included study. Metachronous tumors analyzed RNA sequencing 170) whole-exome 195). Urine samples for immuno-oncology-related proteins 190) tumor-derived DNA...
Abstract Purpose: To investigate the use of plasma and urine DNA mutation analysis for predicting neoadjuvant chemotherapy (NAC) response oncological outcome in patients with muscle-invasive bladder cancer. Experimental Design: Whole-exome sequencing tumor germline was performed 92 treated NAC followed by radical cystectomy (RC). A custom NGS-panel capturing approximately 50 mutations per patient designed used to track mutated urine. total 447 samples, 281 supernatants, 123 pellets collected...
Abstract Purpose: To investigate whether circulating tumor DNA (ctDNA) assessment in patients with muscle-invasive bladder cancer predicts treatment response and provides early detection of metastatic disease. Experimental Design: We present full follow-up results (median follow-up: 68 months) from a previously described cohort neoadjuvant chemotherapy (NAC)-treated who underwent longitudinal ctDNA testing (712 plasma samples). In addition, we performed evaluation 153 samples collected...
Field cancerization is characterized by areas of normal tissue affected mutated clones. Bladder field may explain the development and recurrence bladder cancer be associated with treatment outcomes.