A5090694433- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Extracellular vesicles in disease
- RNA Research and Splicing
- Infant Nutrition and Health
- Prostate Cancer Treatment and Research
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Bone and Dental Protein Studies
- Bladder and Urothelial Cancer Treatments
- Cancer, Lipids, and Metabolism
- Folate and B Vitamins Research
- Digestive system and related health
- Cancer therapeutics and mechanisms
- Muscle metabolism and nutrition
- Autophagy in Disease and Therapy
- COVID-19 Impact on Reproduction
- Heat shock proteins research
- Proteins in Food Systems
- Porphyrin Metabolism and Disorders
- Sirtuins and Resveratrol in Medicine
- Erythrocyte Function and Pathophysiology
- RNA modifications and cancer
- Protein Hydrolysis and Bioactive Peptides
Arla Foods (Denmark)
2018-2025
Central Denmark Region
2025
Aarhus University Hospital
2008-2017
Aarhus University
2012-2017
Hartmann Group (Germany)
2016
Tampere University Hospital
2014
Tampere University
2014
Heinrich Heine University Düsseldorf
2014
Düsseldorf University Hospital
2014
Danish Cancer Society
2005-2013
In the past decade, extracellular vesicles (EVs) have been recognized as potent vehicles of intercellular communication, both in prokaryotes and eukaryotes. This is due to their capacity transfer proteins, lipids nucleic acids, thereby influencing various physiological pathological functions recipient parent cells. While intensive investigation has targeted role EVs different processes, for example, cancer autoimmune diseases, EV-mediated maintenance homeostasis regulation remained less...
MicroRNAs (miRNA) are a class of small noncoding RNAs with important posttranscriptional regulatory functions. Recent data suggest that miRNAs aberrantly expressed in many human cancers and they may play significant roles carcinogenesis. Here, we used microarrays to profile the expression 315 10 normal mucosa samples 49 stage II colon differing regard microsatellite status recurrence disease. Several were differentially between tissue tumor subtypes, miR-145 showing lowest cancer relative...
MicroRNAs (miRNA) are small noncoding RNAs commonly deregulated in cancer. The miR-200 family (miR-200a, -200b, -200c, -141 and -429) miR-205 frequently silenced advanced cancer have been implicated epithelial to mesenchymal transition (EMT) tumor invasion by targeting the transcriptional repressors of E-cadherin, ZEB1 ZEB2. is also known repress miR-200c-141 transcription a negative feedback loop, but otherwise little about regulation miR-205. Recently, silencing was reported stem cells,...
microRNAs (miRNA) are involved in cancer development and progression, acting as tumor suppressors or oncogenes. Here, we profiled the expression of 290 unique human miRNAs 11 normal 106 bladder samples using spotted locked nucleic acid-based oligonucleotide microarrays. We identified several differentially expressed between urothelium different disease stages. miR-145 was found to be most down-regulated compared with normal, miR-21 up-regulated cancer. Furthermore, that significantly...
Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ in size, density and composition. The standard isolation method for exosomes is centrifugation fluid samples, typically at 100,000×g or above. Knowledge the effect discrete ultracentrifugation speeds on purification from different cell types, however, limited.We examined applying differential g-forces ranging 33,000×g to 200,000×g exosome yield purity, using 2 unrelated human lines, embryonic kidney HEK293...
Abstract Exosomes are small secreted vesicles that can transfer their content to recipient cells. In cancer, exosome secretion has been implicated in tumor growth and metastatic spread. this study, we explored the possibility exosomal pathways might discard tumor-suppressor miRNA restricts progression. Secreted characterized from isogenic bladder carcinoma cell lines with differing potential were uncoupled binding target transcripts or AGO2–miRISC complex. cells, observed a relative increase...
Abstract Acquired resistance to classic caspase-mediated apoptosis is a common problem for the treatment of human cancer. Here, we show that siramesine, novel σ-2 receptor ligand, effectively induces caspase-independent programmed cell death in immortalized and transformed cells various origins. Siramesine-treated tumor displayed increased levels reactive oxygen species, lysosomal membrane permeabilization, chromatin condensation, shrinkage detachment cells. Lipid antioxidants (α-tocopherol...
Abstract Vincristine is a microtubule-destabilizing antimitotic drug that has been used in cancer therapy for over 40 years. However, the knowledge on vincristine-induced cell death pathways still sparse. Here, we show vincristine induces dramatic changes lysosomal compartment and sensitizes cells to membrane permeabilization. In HeLa cervix carcinoma cells, induced mitotic arrest massive associated with an early increase volume leakage followed by activation of intrinsic apoptosis program....
Cancer cells secrete soluble factors and various extracellular vesicles, including exosomes, into their tissue microenvironment. The secretion of exosomes is speculated to facilitate local invasion metastatic spread. Here, we used an in vivo metastasis model human bladder carcinoma cell line T24 without capacity its two isogenic derivate lines SLT4 FL3, which form metastases the lungs liver mice, respectively. Cultivation CLAD1000 bioreactors rather than conventional culture flasks resulted...
Abstract Background microRNAs (miRNA) are short, endogenous transcripts that negatively regulate the expression of specific mRNA targets. miRNAs found both in tissues and body fluids such as plasma. A major perspective for use clinical setting is diagnostic plasma markers neoplasia. While abundant tissues, they often scarce For quantification miRNA it therefore importance to a platform with high sensitivity linear performance low concentration range. This motivated us evaluate three commonly...
AbstractA σ-2 receptor ligand siramesine induces lysosomal leakage and cathepsin-dependent death of cancer cells in vitro displays potent anti-cancer activity vivo. The mechanism by which destabilizes lysosomes is, however, unknown. Here, we show that a rapid rise the pH is followed dysfunction. accumulation into cell lysosomes, its ability to destabilize isolated chemical structure as an amphiphilic amine indicate it lysosomotropic detergent. Notably, triggers also substantial Atg6-...
Upregulation of the proto-oncogene plasminogen activator inhibitor-1 (PAI-1) is a common hallmark various solid tumours, but mechanisms controlling its expression are not fully understood. We investigate microRNAs (miRNAs) regulating PAI-1 in panel normal bladder urothelial biopsies, superficial Ta tumours and invasive T1–T4 using microarrays qRT–PCR. The prognostic implications deregulation established by tissue microarray staining non-muscle-invasive tumours. MicroRNA repression assayed...
Aberrant expression of long non-coding RNAs (lncRNAs) has been regarded as a critical component in bladder cancer (BC) and lncRNAs have associated with BC development progression although their overall functional significance is still unclear. The aim our study was to identify novel role carcinogenesis. RNA-sequencing used aberrantly expressed 8 normal 72 samples. We identified 89 that were significantly dys-regulated BC. Five lncRNAs; LINC00958, LINC01296, LINC00355, LNC-CMC1-1 LNC-ALX1-2...
Exosomes have been implicated in tumour progression and metastatic spread. Little is known of the effect mechanical innate immune interactions malignant cell-derived exosomes on endothelial integrity, which may relate to increased extravasation circulating cells and, therefore, spread.Exosomes isolated from non-malignant immortalized HCV-29 isogenic non-metastatic T24 FL3 bladder were characterized by nanoparticle tracking analysis quantitative nanomechanical mapping atomic force microscopy...
Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. These contain biomolecules, including proteins microRNAs (miRNAs). Both circulating EVs miRNAs have received much attention as biomarker candidates for non‐invasive diagnostics. Here we describe a sensitive analytical method isolation subsequent miRNA profiling of epithelial‐derived from blood samples patients with colorectal cancer (CRC). The were isolated by...
Abstract Heat shock protein 70-2 (Hsp70-2) is a chaperone essential for the growth of spermatocytes and cancer cells. Here, we show that Hsp70-2 depletion triggers lysosomal membrane permeabilization cathepsin-dependent cell death identify lens epithelium-derived factor (LEDGF) as an Hsp70-2–regulated guardian stability in human cancer. Knockdown LEDGF cells induces destabilization membranes followed by caspase-independent Bcl-2–resistant death. Accordingly, ectopic stabilizes lysosomes...
Colorectal cancer (CRC) is one of the leading causes deaths in Western countries. A significant number CRC patients undergoing curatively intended surgery subsequently develop recurrence and die from disease. MicroRNAs (miRNAs) are aberrantly expressed cancers appear to have both diagnostic prognostic significance. In this study, we identified novel miRNAs associated with CRC, their possible mechanism action. TaqMan ® Human MicroRNA Array Set v2.0 was used profile expression 667 14 normal...
MicroRNA is a naturally occurring class of non-coding RNA molecules that mediate posttranscriptional gene regulation and are strongly implicated in cellular processes such as cell proliferation, carcinogenesis, survival apoptosis. Consequently there increasing focus on miRNA expression prognostic factors for outcome chemotherapy response. Only approximately 50% patients with bladder cancer respond to chemotherapy. Therefore, predictive markers, miRNAs, can identify subgroups who will benefit...
Abstract Purpose: Available tools for prostate cancer diagnosis and prognosis are suboptimal novel biomarkers urgently needed. Here, we investigated the regulation biomarker potential of GABRE∼miR-452∼miR-224 genomic locus. Experimental Design: GABRE/miR-452/miR-224 transcriptional expression was quantified in 80 nonmalignant 281 tissue samples. promoter methylation determined by methylation-specific qPCR (MethyLight) 35 nonmalignant, 293 [radical prostatectomy (RP) cohort 1] 198 samples (RP...
MicroRNAs (miRNAs) play a critical role in many biological processes and are aberrantly expressed human cancers. Particular miRNAs function either as tumor suppressors or oncogenes appear to have diagnostic prognostic significance. Although numerous dys-regulated colorectal cancer (CRC) only small fraction has been characterized functionally. Using high-throughput functional screening miRNA profiling of clinical samples the present study aims at identifying important for control cellular...
Muscle-invasive bladder cancer (MIBC) represents a highly aggressive tumor type compared to non-muscle-invasive tumors. MIBC is characterized by specific molecular alterations, which may also modulate extracellular tumorigenic effects. Tumor-associated exosomes, especially exosomal miRNAs, are important regulators in the interaction between cells and microenvironment affecting tumor-promoting processes target cells. It analyze whether their patterns reflect characteristics of MIBC. The aim...
ABSTRACT Human milk serves the sole nutritional role for developing infant. During lactation, nano‐sized extracellular vesicles (EVs) in containing a multitude of biologically active components are transferred from mother to offspring. Infant formula (IF) based on cow milk‐derived ingredients has been reported contain reduced levels EVs as compared human milk. There is therefore an unmet need produce large‐scale volumes improve IF composition. Here, we report scalable industrial production...