A5065609799- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Inflammatory Bowel Disease
- Drug-Induced Hepatotoxicity and Protection
- Pancreatitis Pathology and Treatment
- Liver Disease and Transplantation
- Microscopic Colitis
- Cytokine Signaling Pathways and Interactions
- Hepatitis C virus research
- Liver physiology and pathology
- Diabetes and associated disorders
- Organ Transplantation Techniques and Outcomes
- Diet, Metabolism, and Disease
- Immune Cell Function and Interaction
- Pancreatic and Hepatic Oncology Research
- Phagocytosis and Immune Regulation
- Adipokines, Inflammation, and Metabolic Diseases
- Immune Response and Inflammation
- Diet and metabolism studies
- Pharmacological Effects of Natural Compounds
- Biosimilars and Bioanalytical Methods
- Heme Oxygenase-1 and Carbon Monoxide
- Liver Diseases and Immunity
- Hepatocellular Carcinoma Treatment and Prognosis
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
Université Libre de Bruxelles
2003-2024
Hôpital Cardiologique du Haut-Lévêque
2021
Centre Hospitalier Universitaire de Bordeaux
2021
Translational Research in Oncology
2021
Inserm
2020
Université Paris Cité
2020
Délégation Paris 7
2020
Centre de Recherche sur l'Inflammation
2020
Erasmus Hospital
1997-2015
Abstract Immune dysregulations in alcoholic liver diseases are still unclear, especially regarding hepatitis inflammatory burst. Interleukin-17 (IL-17) is known to enhance neutrophil recruitment. We studied the IL-17 pathway cirrhosis and hepatitis. Patients with disease were compared patients chronic C virus (HCV) infection or autoimmune healthy controls. plasma levels peripheral blood mononuclear cell secretion assessed by enzyme-linked immunosorbent assay (ELISA) T phenotype flow...
The role of the anti–inflammatory cytokine interleukin–10 (IL–10) was investigated in mouse model liver injury induced by carbon tetrachloride (CCl 4 ). To address endogenous IL–10 production, acute hepatitis CCl C57Bl/6 gene knock out (KO) and wild–type (WT) mice. After challenge, serum levels tumor necrosis factor–alpha (TNF–α) transforming growth factor–beta 1 (TGF–β1) increased were significantly higher KO mice, whereas IL–6 only slightly compared with WT At histological examination,...
Gut-derived, endotoxin-mediated hepatocellular damage has been postulated to play a crucial role in the pathogenesis of alcohol-induced liver injury rodents. Endotoxins induce production tumor necrosis factor α (TNF-α) by Kupffer cells via Toll-like receptor (TLR) 4 and contribute injury. This study addressed contribution other TLRs ligands alcoholic fatty liver. C57Bl6/J mice were fed modified Lieber-DeCarli diet. Serum aminotransferase measurements, histological analysis, quantification...
Abstract: Only 20% of patients with chronic hepatitis C (CHC) will develop cirrhosis, and fibrosis progression remains highly unpredictable. A recent genome-wide association study identified a genetic variant in the patatin-like phospholipase-3 (PNPLA3) gene (rs738409 C>G) associated steatosis that was further demonstrated to influence severity nonalcoholic fatty liver disease. The aim this assess impact polymorphism on histological damage response antiviral therapy CHC. We recruited 537...
Experimental T-cell-mediated hepatitis induced by concanavalin A (Con A) involves the production of proinflammatory cytokines. Because interleukin (IL)-10 is a potent anti-inflammatory cytokine derived from macrophages and T cells produced within liver, we investigated role IL-10 in modulating hepatotoxicity secretion cytokines following vivo injection Con A. early serum after challenge. Neutralization endogenous monoclonal antibodies (mAbs) increases tumor necrosis factor α (TNF-α) (+111%),...
Interleukin (IL)-10, a potent anti-inflammatory cytokine, limits the severity of acute pancreatitis and downregulates transforming growth factor (TGF)-β release by inflammatory cells on stimulation. Proinflammatory mediators, reactive oxygen species, TGF-β can activate pancreatic stellate their synthesis collagen I III. This study evaluates role endogenous IL-10 in modulation regeneration phase following development fibrosis. knockout (KO) mice C57BL/6 controls were submitted to repeated...
Kupffer cells are thought to mediate most of the deleterious effects liver ischemia-reperfusion injury. The role T and impact resident cell deactivation by interleukin 10 (IL-10) have never been addressed. Using a model ex vivo cold ischemia reperfusion, we assessed injury, tumor necrosis factor (TNF) interferon gamma (IFN-γ) release from livers balb/c mice, nude mice reconstituted with cells, gadolinium pretreated mice. anti-inflammatory cytokine IL-10 was then used define best strategy...
Abstract Experimental T-cell–mediated hepatitis induced by concanavalin A (Con A) involves the production of different cytokines and chemokines is characterized leukocyte infiltration. Because chemokine receptor CCR5 its ligands (CCL3, CCL4, CCL5) regulate chemotaxis activation, we investigated role during Con A–induced liver injury. Serum levels their hepatic transcript were significantly increased after injection, whereas CCR5+ mononuclear cells recruited to liver. CCR5-deficient (CCR5−/−)...
Serum levels and liver expression of CCL2 are increased in patients with alcoholic hepatitis (AH). In an experimental model disease (ALD), was implicated proinflammatory cytokines activation hepatic lipid metabolism, but its role human is currently unknown. a large cohort ALD patients, we analysed plasma their association severity histological lesions. We also studied the relationship between -2518 A > G CCR2 190 A/G polymorphisms ALD. show that compared healthy subjects. AH had...
In mice, body weight is regulated by adipocyte-derived leptin. TNFalpha a critical mediator of inflammation-induced cachexia in Crohn's disease (CD). The regulation leptin poorly understood CD. Pharmacological neutralization with infliximab offers unique opportunity to study TNFalpha-mediated CD patients.We prospectively followed up patients treated (n = 20). Body composition was assessed before and after treatment at 1 4 wk. Serum leptin, IL-6, soluble TNF receptor type II, intercellular...
Oxidative stress plays a major role in the early stage of acute pancreatitis. This study assessed effects N-acetylcysteine (NAC), reduced glutathione (GSH) provider and direct scavenger reactive oxygen intermediates, course pancreatitis mice. Acute (AP) was induced by intraperitoneal (i.p.) injections cerulein. Mice received NAC (1,000 mg/kg, i.p.) every 3 h, starting either 1 h before first cerulein injection (prophylactic group) or after (therapeutic group), i.p. saline for controls....
Hyperammonemia is a common complication of acute and chronic liver diseases. Often accompanied with side effects, therapeutic interventions such as antibiotics or lactulose are generally targeted to decrease the intestinal production absorption ammonia. In this study, we aimed modulate hyperammonemia in three rodent models by administration wild-type Lactobacillus plantarum, genetically engineered ammonia hyperconsuming strain, strain deficient for transporter. Wild-type metabolically L....
Summary In chronic liver disease, high circulating interleukin (IL)-6 contrasts with a poor acute phase response. We evaluated the impact of and IL-6-receptor (IL-6R) forms on IL-6 bioactivity in disease. IL-6, soluble sgp130 levels were assayed plasma from 45 patients alcoholic 84 hepatitis C virus (HCV) infection undergoing transjugular biopsies 15 healthy subjects. IL-6R mRNA was quantified extracts 54 disease or without cirrhosis 18 HCV-infected patients. The effect gp130–Fc fibrinogen...
Vedolizumab (VDZ) is effective as an induction and maintenance treatment for Crohn's disease ulcerative colitis, but, observed with antitumour necrosis factor-α (anti-TNFα) agents, some patients are nonetheless experiencing loss of response.The aim this study was to investigate the impact pharmacokinetics VDZ during on long-term response.This focused a single cohort 103 inflammatory bowel treated VDZ. trough levels (TLs) were measured by enzyme-linked immunosorbent assay (n=536 samples),...
Weekly injections of Concanavalin A (Con A) were performed in BALB/c mice to evaluate the pattern cytokine production and liver injury. High serum levels tumor necrosis factor α (TNF-α), interleukin 2 (IL-2), IL-4, interferon gamma (IFN-γ) found after first Con but rapidly decreased from third injection. Conversely, IL-10 repeated challenge increased by 7 times week 1 20. In vivo depletion studies indicated that CD4 + T cells are essential production. Hepatocyte was only observed whereas...
Acute pancreatitis (AP) is an inflammatory disease involving the production of different cytokines and chemokines characterized by leukocyte infiltration. Because chemokine receptor CCR5 its ligands [the CC CCL3/MIP-1α, CCL4/MIP-1β, CCL5/regulated upon activation, normal T cell expressed secreted (RANTES)] regulate chemotaxis we investigated expression role in experimental AP mice. was induced hourly intraperitoneal injections cerulein CCR5-deficient (CCR5 −/− ) or wild-type (WT) Induction...
Acute pancreatitis (AP) is an inflammatory disease in which the regulatory pathways are not clearly elucidated. Activation of interleukin 1β (IL-1β) and immunomodulation via MyD88, first signaling molecule ST2 pathway, seem to be involved. Because IL-33, ligand, IL-1 family member acts as alarmin, we explored pathway human mouse AP. Soluble was assayed by enzyme-linked immunosorbent assay (ELISA) plasma 44 patients admitted for The levels soluble increased early during AP correlated with...
Abstract Extracellular release of HMGB1 contributes to acetaminophen-induced liver injury. acts as a danger-associated molecular patterns during this toxic process but the mechanisms action and targeted cells are incompletely defined. Here we studied, in vitro , role amplifying hepatocyte necrosis process. Using cultured HepaRG cells, primary human hepatocytes selective chemical inhibitors evaluated toxicity. We confirmed that addition acetaminophen induced cell death release. showed...
Infliximab (IFX) is indicated for the treatment of inflammatory bowel diseases (IBD). Nevertheless, loss response (LOR) to IFX reported in up 10% 30% patients within first year treatment. Our objective was evaluate impact pharmacokinetics at induction on failure.This a longitudinal cohort study 269 with IBD treated single center. A total 2331 blood samples were prospectively collected from 2007 until March 2015 retrospective analysis clinical data. trough levels (TLs) measured by...