A5011682597- Amyotrophic Lateral Sclerosis Research
- Neurogenesis and neuroplasticity mechanisms
- Electrolyte and hormonal disorders
- Salivary Gland Disorders and Functions
- Pluripotent Stem Cells Research
- Neurogenetic and Muscular Disorders Research
- Metabolism, Diabetes, and Cancer
- Immune Cell Function and Interaction
- Diabetes and associated disorders
- Spinal Cord Injury Research
- Prion Diseases and Protein Misfolding
- Neuroscience of respiration and sleep
- Pancreatic function and diabetes
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Mesenchymal stem cell research
- Nerve injury and regeneration
- Apelin-related biomedical research
- Long-Term Effects of COVID-19
- Diet, Metabolism, and Disease
- IL-33, ST2, and ILC Pathways
- Advanced X-ray Imaging Techniques
- Congenital Diaphragmatic Hernia Studies
- biodegradable polymer synthesis and properties
- Eosinophilic Esophagitis
University of Namur
2016-2025
Namur Research Institute for Life Sciences
2011-2023
Université Libre de Bruxelles
2004-2016
Erasmus Hospital
2014-2016
Jefferson Hospital for Neuroscience
2012-2014
Thomas Jefferson University
2012-2014
VIB-UAntwerp Center for Molecular Neurology
2014
Gut-derived, endotoxin-mediated hepatocellular damage has been postulated to play a crucial role in the pathogenesis of alcohol-induced liver injury rodents. Endotoxins induce production tumor necrosis factor α (TNF-α) by Kupffer cells via Toll-like receptor (TLR) 4 and contribute injury. This study addressed contribution other TLRs ligands alcoholic fatty liver. C57Bl6/J mice were fed modified Lieber-DeCarli diet. Serum aminotransferase measurements, histological analysis, quantification...
Abstract Current literature suggests that T cells recognizing antigen on mature dendritic (DC) differentiate into effector whereas tolerance is induced when presented by immature DC. We investigated the consequences of interactions between or lipopolysaccharide‐matured DC and CD4 pos lymphocytes in absence foreign antigen. While did not induce significant cell activation, we observed a fraction cultured with autologous displayed phenotypic features activation produced IL‐2, IFN‐γ, IL‐10...
Abrupt osmotic changes during rapid correction of chronic hyponatremia result in demyelinative brain lesions, but the sequence events linking to myelin loss is not yet understood. Here, a rat model demyelination syndrome, we found that massive astrocyte death occurred after hyponatremia, delineating regions future loss. Astrocyte caused disruption astrocyte-oligodendrocyte network, rapidly upregulated inflammatory cytokines genes, and increased serum S100B, which predicted clinical...
A major portion of spinal cord injury (SCI) cases affect midcervical levels, the location phrenic motor neuron (PhMN) pool that innervates diaphragm. While initial trauma is uncontrollable, a valuable opportunity exists in hours to days following SCI for preventing PhMN loss and consequent respiratory dysfunction occurs during secondary degeneration. One primary causes excitotoxic cell death due dysregulation extracellular glutamate homeostasis. GLT1, mainly expressed by astrocytes,...
Abstract Experimental T-cell–mediated hepatitis induced by concanavalin A (Con A) involves the production of different cytokines and chemokines is characterized leukocyte infiltration. Because chemokine receptor CCR5 its ligands (CCL3, CCL4, CCL5) regulate chemotaxis activation, we investigated role during Con A–induced liver injury. Serum levels their hepatic transcript were significantly increased after injection, whereas CCR5+ mononuclear cells recruited to liver. CCR5-deficient (CCR5−/−)...
The transplantation of neural stem cells (NSCs) is a challenging therapeutic strategy for the treatment neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS). To provide insight into potential intravenous delivery NSCs, we evaluated NSCs marked with green fluorescent protein to central nervous system (CNS) via tail vein injections in an ALS model. injected cell fates were followed 1, 3, and 7 days after transplantation. highest efficiency CNS was found symptomatic (up 13%),...
Contusion-type cervical spinal cord injury (SCI) is one of the most common forms SCI observed in patients. In particular, injuries targeting C3-C5 region affect pool phrenic motor neurons (PhMNs) that innervates diaphragm, resulting significant and often chronic respiratory dysfunction. Using a previously described rat model unilateral midcervical C4 contusion with Infinite Horizon Impactor, we have characterized early time course PhMN degeneration consequent deficits following injury, as...
A primary cause of morbidity and mortality following cervical spinal cord injury (SCI) is respiratory compromise, regardless the level trauma. In particular, SCI at mid-cervical regions targets degeneration both descending bulbospinal axons cell bodies phrenic motor neurons, resulting in deficits function diaphragm, muscle inspiration. Contusion-type trauma to one most common forms human SCI; however, few studies have evaluated contusion animal models or characterized consequent...
Human induced pluripotent stem cells (iPSCs) offer hope for personalized regenerative cell therapy in amyotrophic lateral sclerosis (ALS). We analyzed the fate of human iPSC-derived neural progenitors transplanted into spinal cord wild-type and transgenic rats carrying a mutated SOD1(G93A) gene. The aim was to follow survival differentiation until day 60 post-transplantation two different vivo environments, one being ALS-like. efficiently engrafted adult survived at high numbers. Different...
To investigate the role of interleukin (IL)-33-ST2 axis in pathophysiology primary Sjögren's syndrome (pSS).Serum levels IL-33 and sST2 were determined by ELISA. The expression ST2 was investigated salivary glands (SG) immunohistochemistry. PBMC isolated stimulated with IL-33, IL-12 IL-23 cytokine profile response examined flow cytometry. Intracellular detection IFNγ IL-17 performed cytometry.Serum increased pSS patients compared controls systemic lupus erythematosus. Expression upregulated...
Biomarker-based tracking of human stem cells xenotransplanted into animal models is crucial for studying their fate in the field cell therapy or tumor xenografting.Using immunohistochemistry and situ hybridization, we analyzed expression three human-specific biomarkers: Ku80, mitochondria (hMito) Alu.We showed that hMito Alu biomarkers are broadly expressed tissues with no low cross-reactivity toward rat, mouse pig tissues. In vitro, demonstrated stable over time does not change along...
Amyotrophic lateral sclerosis (ALS) is the most common motor neuron (MN) disease in adults with no curative treatment. Neurofilament (NF) level patient' fluids have recently emerged as prime biomarker of ALS progression, while NF accumulation MNs patients oldest and one best pathological hallmarks. However, way accumulations could lead to MN degeneration remains unknown. To assess study impact on MNs, we compared derived from induced pluripotent stem cells (iPSC) carrying mutations C9orf72,...
Recent estimates consider that Long COVID affects 400 million people worldwide, of which <10% recover after two years. Predictive biomarkers and effective therapies are urgently needed for the clinical management COVID. Neurological manifestations (poor memory, brain fog) frequent, while neurodamage neuroinflammatory markers UCHL1, GFAP NFL were found to be increased in some but not all cohorts. However, a direct link between major disease mechanisms, viral persistence coagulation...
Background: Tubulointerstitial hypoxia is a key factor for lupus nephritis progression to end-stage renal disease. Numerous aquaporins (AQPs) are expressed by tubules and essential their proper functioning. The aim of this study characterize the tubular expression AQP1, AQP2 AQP3, which could provide better understanding tubulointerstitial stress during nephritis. Methods: This retrospective monocentric was conducted at Erasme-HUB Hospital. We included 37 samples 9 healthy collected between...
A murine model mimicking the human osmotic demyelination syndrome (ODS) revealed with histology demyelinated alterations in relay posterolateral (VPL) and ventral posteromedial (VPM) thalamic nuclei 12 h 48 after chronic hyponatremia due to a fast reinstatement of osmolality. Abnormal expression astrocyte markers ALDHL1 GFAP immunohistochemistry these ODS altered zones, prompted aims verify both protoplasmic fibrillar astrocytes ultrastructure those changes other associated subcellular...
C. Nicaise, D. Mitrecic and R. Pochet (2011) Neuropathology Applied Neurobiology37, 179–188Brain spinal cord affected by amyotrophic lateral sclerosis induce differential growth factors expression in rat mesenchymal neural stem cells Stem cell research raises hopes for incurable neurodegenerative diseases. In (ALS), affecting the motoneurones of central nervous system (CNS), cell-based therapy aims to replace dying host transplantation disease-affected regions. Moreover, transplanted can...
Abstract Onset of motoneuron death characterizing amyotrophic lateral sclerosis (ALS) is closely linked to modified astrocytic and glial environments. Here, we show that in the spinal cord from transgenic rat overexpressing mutated human SOD1, aquaporin‐4 mRNA protein are specifically overexpressed gray matter at end stage disease. Immunohistochemistry double immunofluorescence allowed detect, ALS rat, increased surrounding both vessel perikarya. The use pre‐embedding immunohistochemistry...
Hyperammonemia is a common complication of acute and chronic liver diseases. Often accompanied with side effects, therapeutic interventions such as antibiotics or lactulose are generally targeted to decrease the intestinal production absorption ammonia. In this study, we aimed modulate hyperammonemia in three rodent models by administration wild-type Lactobacillus plantarum, genetically engineered ammonia hyperconsuming strain, strain deficient for transporter. Wild-type metabolically L....