A5046902998- Vitamin C and Antioxidants Research
- Vitamin K Research Studies
- Electrolyte and hormonal disorders
- Pediatric Hepatobiliary Diseases and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Gallbladder and Bile Duct Disorders
- RNA Interference and Gene Delivery
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Enzyme function and inhibition
- Antioxidant Activity and Oxidative Stress
- DNA and Nucleic Acid Chemistry
- Cardiomyopathy and Myosin Studies
- Erythrocyte Function and Pathophysiology
- Drug Transport and Resistance Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Calcium Carbonate Crystallization and Inhibition
- Neurobiology and Insect Physiology Research
- Neuropeptides and Animal Physiology
- HIV/AIDS drug development and treatment
- Burn Injury Management and Outcomes
- Folate and B Vitamins Research
- Aquaculture Nutrition and Growth
- Effects and risks of endocrine disrupting chemicals
Northumbria University
2011-2025
University of Namur
2015-2025
St. George's University
2009-2025
Northeast Ohio Medical University
1995-2024
SUNY Upstate Medical University
1979-2024
Université Libre de Bruxelles
2023
KES International
2013
Newcastle University
2010
St George's, University of London
2009
Namur Research Institute for Life Sciences
2008
A murine model mimicking the human osmotic demyelination syndrome (ODS) revealed with histology demyelinated alterations in relay posterolateral (VPL) and ventral posteromedial (VPM) thalamic nuclei 12 h 48 after chronic hyponatremia due to a fast reinstatement of osmolality. Abnormal expression astrocyte markers ALDHL1 GFAP immunohistochemistry these ODS altered zones, prompted aims verify both protoplasmic fibrillar astrocytes ultrastructure those changes other associated subcellular...
The purpose of this investigation was to determine whether heavy-resistance exercise training alters the skeletal muscle fiber composition young rats. Ten male Long Evans rats (3 wk old) were trained lift progressively heavier weights, which secured rats' tails, while they ascended a 40-cm 90 degree mesh incline 20 times/day 5 days/wk for food reward. After 8 training, lifted 406 +/- 19 (SD) g in addition their body weight (261 9 g). Compared with 10 sedentary pair-fed rats, no hypertrophy...
Abstract Scanning and transmission electron microscopy fluorescence light were employed to characterize the cytotoxic effects of vitamin C (VitC), K 3 (VitK ) or a VitC:VK combination on human bladder carcinoma cell line (T24) following 1‐h 2‐h treatment. T24 cells exposed VitC alone exhibited membranous damage (blebs endoplasmic extrusions, elongated microvilli). VitK ‐treated displayed greater membrane enucleation than those treated with as well cytoplasmic defects characteristic...
A human bladder carcinoma cell line RT4 was sham-treated with buffer or treated ascorbate (VC) alone, menadione alone (VK(3)), a combination of ascorbate:menadione (VC+VK(3)) for 1, 2, and 4 h. Cytotoxic damage found to be treatment-dependent in this sequence: VC+VK(3)>VC>VK(3)>sham. The combined treatment induced the greatest oxidative stress, early tumor injury affecting cytoskeletal architecture contributing self-excisions pieces cytoplasm freed from organelles. Additional damage,...
Human prostate cancer cells (DU145) implanted into nude mice are deficient in DNase activity. After administration of a vitamin C/vitamin K(3) combination, both alkaline (DNase I) and acid II) activities were detected cryosections with histochemical lead nitrate technique. Alkaline activity appeared 1 hr after administration, decreased slightly until 2 hr, disappeared by 8 treatment. Acid reached its highest levels between 4 maintained 24 Methyl green staining indicated that expression was...
Scanning and transmission electron microscopy were employed to further characterize the cytotoxic effects of a ascorbic acid/menadione (or vitamin C/vitamin K3) combination on human bladder carcinoma T24 cell line. Following 1-h treatment cells display membrane mitochondrial defects as well excision cytoplasmic fragments that contain no organelles. These continuous self-excisions reduce size. Concomitant, nuclear changes, chromatin disassembly, nucleolar condensation fragmentation, decreased...
Abstract The osmotic demyelination syndrome (ODS) is a non‐primary inflammatory disorder of the central nervous system myelin that often associated with precipitous rise serum sodium concentration. To investigate physiopathology ODS in vivo , we generated novel murine model based on abrupt correction chronic hyponatremia. Accordingly, mice developed impairments brainstem auditory evoked potentials and grip strength. At 24 hr post‐correction, oligodendrocyte markers (APC Cx47) were...
Scanning (SEM) and transmission electron microscopy (TEM) were used to characterize the cytotoxic effects of ascorbate (VC), menadione (VK3), or a VC:VK3 combination on human prostate carcinoma cell line (DU145) following 1-h vitamin treatment subsequent 24-h incubation in culture medium. Cell alterations examined by light treatment-dependent with VC + VK3 >VK3 > Sham. Oxidative stress-induced damage was found most organelles. This report describes injuries tumor nucleus (chromatin...
Vitamins C, K 3 (VC, VK ) and a VC/VK combination with VC:VK ratio of 100:1 were assayed for their antitumour activity against two human prostatic carcinoma cell lines. Co‐administration the vitamins enhanced 5‐ to 20‐fold even 1 h exposure time. While exogenous catalase destroyed activity, hydrogen peroxide‐induced lipid peroxidation was negligible. Analysis cellular ATP thiol levels as well DNA protein synthesis revealed: transient increase in production, decrease synthesis, an levels....