A5083435709- Ion Transport and Channel Regulation
- Salivary Gland Disorders and Functions
- Pancreatic function and diabetes
- Neuropeptides and Animal Physiology
- Regulation of Appetite and Obesity
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Biochemical Analysis and Sensing Techniques
- Neonatal Respiratory Health Research
- Diet, Metabolism, and Disease
- Virus-based gene therapy research
- Ion channel regulation and function
- Heart Failure Treatment and Management
- Electrolyte and hormonal disorders
- Retinal Diseases and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- 3D Printing in Biomedical Research
- Diabetes and associated disorders
- Cardiac electrophysiology and arrhythmias
- Retinal Development and Disorders
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
- Caveolin-1 and cellular processes
- Retinal and Macular Surgery
- Galectins and Cancer Biology
Université Libre de Bruxelles
2016-2025
Erasmus Hospital
2003
National Institute of Dental and Craniofacial Research
1995-2002
National Institutes of Health
1995-2002
Johns Hopkins University
1997
Abstract The endogenous ligand for the GH secretagogue receptor is ghrelin, a peptide recently purified from stomach. Ghrelin n-octanoylated on Ser3 residue, and this modification essential its interaction with receptor. degradation of ghrelin by rat human serum, commercial enzymes, tissues homogenates was analyzed combining HPLC mass spectrometry. In desoctanoylated, without proteolysis. desoctanoylation significantly reduced phenylmethylsulfonyl fluoride, serine proteases esterases...
A replication-deficient, recombinant adenovirus encoding human aquaporin-1 (hAQP1), the archetypal water channel, was constructed. This virus, AdhAQP1, directed hAQP1 expression in several epithelial cell lines vitro . In polarized MDCK monolayers, localized apical and basolateral plasma membranes. Fluid movement across monolayers infected by AdhAQP1 response to an osmotic gradient ≈4-fold that seen with uninfected or a control virus. When administered rat submandibular glands retrograde...
Abstract This study aims to assess kinetic modelling of the solid–liquid extraction process total polyphenolic compounds (TPC) from apple pomace (AP). In this regard, we investigated effects temperature and solvent (i.e. water, ethanol, acetone) on TPC over various periods. The highest yield 11.1 ± 0.49 mg gallic acid equivalent (GAE)/g db (dry basis) was achieved with a mixture 65% acetone–35% water (v/v) at 60 °C. kinetics solvent-based processes were assessed via first-order second-order...
The Trp gene product has been proposed as a candidate protein for the store-operated Ca 2+ channel, but protein(s) not identified in any nonexcitable cell. We report here cloning of rat brain Trp1β cDNA and detection immunolocalization endogenous expressed Trp1 protein. A 400-bp product, with >95% homology to mouse Trp1, was amplified from submandibular gland RNA. Rat-specific primers were used full-length (rTrp1), encoding 759 amino acids. Northern blot analysis demonstrated transcript...
Ghrelin, a peptide hormone produced predominantly by the stomach, stimulates food intake and GH secretion. The Ser(3) residue of ghrelin is mainly modified n-octanoic acid. In human bloodstream, circulates in two forms: octanoylated desacylated. We previously demonstrated that desoctanoylated serum butyrylcholinesterase (EC 3.1.1.8) other esterase(s), whereas rat serum, only carboxylesterase 3.1.1.1) involved. aims this study were to determine role lipoprotein-associated enzymes...
Background Analysis of gene expression at the mRNA level, using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), mandatorily requires reference genes (RGs) as internal controls. However, increasing evidences have shown that RG may vary considerably under experimental conditions. We sought for an appropriate panel RGs to be used in 3T3-L1 cell line model during their terminal differentiation into adipocytes. To this end, levels a seven widely mRNAs were...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew evidence for a membrane-bound pathway in hormone receptor bindingLuis Moroder, Roberta Romano, Wolfgang Guba, Dale F. Mierke, Horst Kessler, Christine Delporte, Jacques Winand, and Jean ChristopheCite this: Biochemistry 1993, 32, 49, 13551–13559Publication Date (Print):December 14, 1993Publication History Published online1 May 2002Published inissue 14 December...