Sorbitol is a low-calorie sugar alcohol that largely used as an ingredient in the food industry, based on its sweetness and high solubility. Here, we investigated capacity of Lactobacillus plantarum, lactic acid bacterium found many fermented products gastrointestinal tract mammals, to produce sorbitol from fructose-6-phosphate by reverting catabolic pathway mutant strain deficient for both l- d-lactate dehydrogenase activities. The two sorbitol-6-phosphate (Stl6PDH) genes (srlD1 srlD2)...

In many streptococci, competence for natural DNA transformation is regulated by the Rgg-type regulator ComR and pheromone ComS, which sensed intracellularly. We compared ComRS systems of four model streptococcal species using in vitro silico approaches, to determine mechanism ComRS-dependent regulation competence. all investigated, was shown be proximal transcriptional activator expression key genes. Efficient binding strictly dependent on presence (C-terminal ComS octapeptide), contrast...

Lactobacillus plantarum is a lactic acid bacterium that produces d- and l-lactate using stereospecific NAD-dependent lactate dehydrogenases (LdhD LdhL, respectively). However, reduction of glycolytic pyruvate by LdhD not the only pathway for d-lactate production since mutant defective in this activity still both isomers (T. Ferain, J. N. Hobbs, Jr., Richardson, Bernard, D. Garmyn, P. Hols, E. Allen, Delcour, Bacteriol. 178:5431-5437, 1996). Production species has been shown to be connected...

ABSTRACT The pyruvate oxidase gene ( poxB ) from Lactobacillus plantarum Lp80 was cloned and characterized. Northern blot primer extension analyses revealed that transcription of is monocistronic under the control a vegetative promoter. mRNA expression strongly induced by aeration repressed glucose. Moreover, blotting performed at different stages growth showed maximal in early stationary phase when glucose exhausted. Primer vivo footprint repression mediated CcpA binding to cre site...

Whooping cough is a highly contagious respiratory disease caused by

Cases of congenital disorders glycosylation (CDG) have been associated with specific mutations within the gene encoding human Golgi TMEM165 (transmembrane protein 165), belonging to UPF0016 (uncharacterized family 0016), a secondary ion transporters. To date, members this reported be involved in calcium, manganese, and pH homeostases. Although it has suggested that cation transport activity, direct evidence for its Ca2+- Mn2+-transporting activities is still lacking. Here, we functionally...

ABSTRACT In addition to the previously characterized pyruvate oxidase PoxB, Lactobacillus plantarum genome encodes four predicted oxidases (PoxC, PoxD, PoxE, and PoxF). Each gene was individually inactivated, only knockout of poxF resulted in a decrease activity under tested conditions. We show here that L. has two major oxidases: PoxB PoxF. Both are involved lactate-to-acetate conversion early stationary phase aerobic growth regulated by carbon catabolite repression. A strain devoid...

NAD-independent lactate dehydrogenases are commonly thought to be responsible for utilization during the stationary phase of aerobic growth in Lactobacillus plantarum. To substantiate this view, we constructed single and double knockout mutants corresponding genes, loxD loxL. Lactate-to-acetate conversion was not impaired these strains, while it completely blocked deficient NAD-dependent dehydrogenase activities, encoded by ldhD ldhL genes. We conclude that but involved process.

Hyperammonemia is a common complication of acute and chronic liver diseases. Often accompanied with side effects, therapeutic interventions such as antibiotics or lactulose are generally targeted to decrease the intestinal production absorption ammonia. In this study, we aimed modulate hyperammonemia in three rodent models by administration wild-type Lactobacillus plantarum, genetically engineered ammonia hyperconsuming strain, strain deficient for transporter. Wild-type metabolically L....

South Sudan is focusing on the development of large hydropower (LHP) to urgently improve access electricity in country. However, LHP investments are expensive and take many years construct. SHP can be deployed as an alternative developments they constructed a shorter time deliver locations need. This study aims assess run-of-river (ROR) potential Central Equatoria State (CES) Sudan. first The main contribution this research produce dataset showing geographical generation capacities sites....

Abstract “Giving the cells exactly what they need, when need it” is core idea behind proposed bioprocess control strategy: operating based on physiological behavior of microbial population rather than exclusive monitoring environmental parameters. We are envisioning to achieve this through use genetically encoded biosensors combined with online flow cytometry (FCM) obtain a time‐dependent “physiological fingerprint” population. developed biosensor glnA promoter ( glnAp ) and applied it for...

ABSTRACT Lactobacillus plantarum produces peptidoglycan precursors ending in d -lactate instead of -alanine, making the bacterium intrinsically resistant to vancomycin. The ligase Ddl L. plays a central role this specificity by synthesizing -alanyl- depsipeptides that are added precursor peptide chain enzyme MurF. Here we show also encodes -Ala- -Ala dipeptidase, Aad, which eliminates -alanine dipeptides produced ligase, thereby preventing their incorporation into precursors. Although...

Lactobacillus plantarum is a lactic acid bacterium that produces racemic mixture of l- and d-lactate from sugar fermentation. The interconversion lactate isomers performed by racemase (Lar) transcriptionally controlled the l-/d-lactate ratio maximally induced in presence l-lactate. We previously reported Lar activity depends on expression two divergently oriented operons: (i) larABCDE operon encodes nickel-dependent (LarA), its maturases (LarBCE), channel (LarD), (ii) larR(MN)QO...

Abstract A high cell density fed‐batch process was developed for production of recombinant CRM197, a non‐toxic mutant diphtheria toxin widely used as carrier in polysaccharide‐protein conjugate vaccines. Fully soluble CRM197 obtained yields and with an authentic N‐terminus, by targeting the protein to periplasm Escherichia coli using Signal Recognition Particle (SRP)‐dependent signal sequence FlgI. Response Surface Methodology (RSM) optimize set‐points key parameters (pH feed rate at...

Combining production of antibacterial compounds and uptake DNA material released by dead cells, competence is one the most efficient survival strategies in streptococci. Yet, this powerful tactic energy consuming reprograms metabolism to such an extent that cell proliferation transiently impaired.

South Sudan is expansive and sparsely populated with over 80% of the population living in rural areas. The country has no national grid connecting its cities towns, thus making areas "good candidates" for stand-alone renewable energy systems. This study was conducted to determine technical feasibility economic viability a photovoltaic (PV) system compared diesel generator. A technoeconomic model developed forecast performance PV system. initially designed using IEEE Recommended Practice...

ABSTRACT Escherichia coli BLR(DE3) is a commercially available recA -deficient derivative of BL21(DE3), one the most widely used strains for recombinant protein expression. Here, we present full-genome sequence and highlight additional differences with its parent strain BL21(DE3) which were previously unreported but may affect physiology.