A5062098632- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Legume Nitrogen Fixing Symbiosis
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Probiotics and Fermented Foods
- Photosynthetic Processes and Mechanisms
- DNA Repair Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Vibrio bacteria research studies
- Cancer Research and Treatments
- Amino Acid Enzymes and Metabolism
- ATP Synthase and ATPases Research
- Plant biochemistry and biosynthesis
- Microtubule and mitosis dynamics
- Peptidase Inhibition and Analysis
- Reproductive Biology and Fertility
- Endoplasmic Reticulum Stress and Disease
- Protist diversity and phylogeny
- Genetics and Neurodevelopmental Disorders
- Escherichia coli research studies
- Hippo pathway signaling and YAP/TAZ
Newcastle University
2014-2024
University of Newcastle Australia
2007-2023
College of Mount Saint Vincent
2020
Saint Vincent Health System
2020
University of Oxford
1993-2006
Michigan State University
1997
Southern Methodist University
1993
Bacillus subtilis is the best-characterized member of Gram-positive bacteria. Its genome 4,214,810 base pairs comprises 4,100 protein-coding genes. Of these genes, 53% are represented once, while a quarter corresponds to several gene families that have been greatly expanded by duplication, largest family containing 77 putative ATP-binding transport proteins. In addition, large proportion genetic capacity devoted utilization variety carbon sources, including many plant-derived molecules. The...
The twin‐arginine translocation (Tat) system targets cofactor‐containing proteins across the Escherichia coli cytoplasmic membrane via distinct signal peptides bearing a motif. In this study, we have analysed mechanism and capabilities of E. Tat using green fluorescent protein (GFP) fused to peptide TMAO reductase (TorA). Fractionation studies fluorescence measurements demonstrate that GFP is exported periplasm where it fully active. Export almost totally blocked in tat deletion mutants,...
The characteristic shape of bacterial cells is mainly determined by the cell wall, synthesis which orchestrated penicillin-binding proteins (PBPs). Rod-shaped bacteria have two distinct modes wall synthesis, involved in elongation and division, are believed to employ different sets PBPs. A long-held question has been how these growth co-ordinated space time. We now identified division protein, EzrA, a newly discovered GpsB, as key players elongation-division cycle Bacillus subtilis....
ABSTRACT Cell division in bacteria is carried out by about a dozen proteins which assemble at midcell and form complex known as the divisome. To study dynamics temporal hierarchy of divisome assembly Bacillus subtilis , we have examined vivo localization pattern set fused to green fluorescent protein germinating spores vegetative cells. Using time series time-lapse microscopy, show that FtsZ ring assembles early concomitantly with FtsA, ZapA, EzrA. After delay least 20% cell cycle, second...
Cell division in bacteria is governed by a complex cytokinetic machinery which the key player tubulin homologue, FtsZ. Most rod-shaped divide precisely at mid-cell between segregated sister chromosomes. Selection of correct site for cell thought to be determined two negative regulatory systems: nucleoid occlusion system, prevents vicinity chromosomes, and Min inappropriate poles. In Bacillus subtilis recruitment inhibitor MinCD poles depends on DivIVA, these proteins were sufficient...
The bacterial actin homologue MreB plays a key role in cell morphogenesis. In Bacillus subtilis is essential under normal growth conditions and mreB mutants are defective the control of diameter. However, precise still unclear. Analysis lethal phenotypic consequences disruption revealed an unusual bulging phenotype that precedes death. A similar was seen wild-type cells at very low Mg(2+) concentrations. We found inactivation major bi-functional penicillin-binding protein (PBP) PBP1 B....
Bacterial growth and cell division requires precise spatiotemporal regulation of the synthesis remodelling peptidoglycan layer that surrounds cytoplasmic membrane. GpsB is a cytosolic protein affects wall by binding mini-domains synthases to ensure their correct subcellular localisation. Here, we describe critical structural features for interaction with from three bacterial species (Bacillus subtilis, Listeria monocytogenes Streptococcus pneumoniae) suggest importance viability in L. S....
DNA replication in bacteria is carried out by a multiprotein complex, which thought to contain only one essential polymerase, specified the dnaE gene Escherichia coli and polC Bacillus subtilis . genome analysis has revealed another polymerase gene, BS , homologous We show that, B. for cell viability elongation step of replication, as polC, we conclude that there are two different polymerases at fork was previously observed eukaryotes. appears be involved synthesis lagging strand associated...
Cell morphogenesis in most bacteria is governed by spatiotemporal growth regulation of the peptidoglycan cell wall layer. Much known about synthesis but its turnover hydrolytic enzymes much less well understood. Bacillus subtilis has a multitude such enzymes. Two best characterized are CwlO and LytE: cells lacking both have lethal block elongation. Here we show that activity regulated an ABC transporter, FtsEX, which required for elongation, unlike division as Escherichia coli. Actin-like...
We have characterized the role of penicillin‐binding protein PBP 2B in cell division Bacillus subtilis . shown that depletion results an arrest division, but this is slow, probably because relatively stable. 2B‐depleted filaments contained, at about their mid‐points, structures resembling partially formed septa, into which most, if not all, proteins had assembled. Although clearly deficient wall material, membrane invagination seemed to continue, indicating and ingrowth can be uncoupled. At...
Mutations in the spoIVA locus of Bacillus subtilis abolish cortex synthesis and interfere with assembly spore coat. We have characterized cloned terms its physical structure regulation during sporulation. The contains a single gene capable encoding an acidic protein 492 amino acids (molecular weight, 55,174). is transcribed from sigma E-dependent promoter soon after formation septum. A genetic test indicated that expression only necessary mother cell compartment for mature spore. This,...
We have identified the Bacillus subtilis homologue of essential cell division gene, ftsL , Escherichia coli . Repression in a strain engineered to carry conditional promoter results filamentation, with near immediate arrest division. The filaments show no sign invagination, indicating that is blocked at an early stage. FtsL also shown be required for septation during sporulation, and depletion blocks activation but not synthesis prespore‐specific sigma factor, σ F Immunofluorescence...
The pbpB gene, which encodes penicillin-binding protein (PBP) 2B of Bacillus subtilis, has been cloned, sequenced, mapped, and mutagenized. sequence PBP places it among the class B high-molecular-weight PBPs. It appears to contain three functional domains: an N-terminal domain homologous corresponding other PBPs, a domain, lengthy carboxy extension. noncleaved signal at its N terminus that presumably serves as anchor in cell membrane. Previous studies led hypothesis is required for both...
DivIVA is a well-conserved coiled-coil protein present in most Gram-positive bacteria and has been implicated division site selection, peptidoglycan biosynthesis sporulation. proteins bind lipid membranes characteristically accumulate at curved membrane areas, i.e. the cell poles site, to which they recruit various interaction partners. We have studied role of this morphogen human pathogen Listeria monocytogenes our results suggest novel mechanism by contributes division. Contrary...
Gram-positive bacterial cells are protected from the environment by a cell envelope that is comprised of thick layer peptidoglycan maintains shape and teichoic acid polymers whose biological function remains unclear. In Bacillus subtilis, loss all class A penicillin-binding proteins (aPBPs), which in synthesis, conditionally lethal. Here, we show this lethality associated with an alteration lipoteichoic acids (LTAs) accumulation major autolysin LytE wall. Our analysis provides further...
SUMMARY: The spo-87 mutation is one of two sporulation mutations originally used to define the spo0J locus Bacillus subtilis. We now show that it blocks after completion prespore engulfment (stage III). Surprisingly, operon expressed vegetatively, probably from a σA-dependent promoter, and its expression shut down at transcriptional level about onset sporulation. DNA sequencing reveals defined by spo-87, which we designate spoIIIJ, consists bicistronic operon. However, only first gene...
About 11 essential proteins assemble into a ring structure at the surface of cell to bring about cytokinesis in bacteria. Several these have their major domains located outside membrane, forming an assembly that we call outer (OR). Previous work on division Bacillus subtilis has shown four OR proteins-FtsL, DivIC, DivIB, and PBP 2B-are interdependent for assembly. This contrasts with mainly linear pathway equivalent Escherichia coli. Here show nature B. could be due effects FtsL DivIC...