A5076593604- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Glycosylation and Glycoproteins Research
- Pneumonia and Respiratory Infections
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- Escherichia coli research studies
- Enzyme Structure and Function
- Streptococcal Infections and Treatments
- Carbohydrate Chemistry and Synthesis
- Antimicrobial Resistance in Staphylococcus
- Legume Nitrogen Fixing Symbiosis
- Protein Structure and Dynamics
- Peptidase Inhibition and Analysis
- Bacterial Infections and Vaccines
- Biochemical and Structural Characterization
- Helicobacter pylori-related gastroenterology studies
- Enzyme Production and Characterization
- Salmonella and Campylobacter epidemiology
- Probiotics and Fermented Foods
- Amino Acid Enzymes and Metabolism
- Reproductive tract infections research
Newcastle University
2016-2025
The University of Queensland
2023-2025
University of Newcastle Australia
2013-2024
University of Tübingen
2004-2014
Institut de Biologie Structurale
2008-2010
Institut de Chimie
2008-2010
University of Liège
2008-2010
Université Joseph Fourier
2008-2010
Centre National de la Recherche Scientifique
2008-2010
CEA Grenoble
2008-2010
Summary Staphylococcus species belong to one of the few bacterial genera that are completely lysozyme resistant, which greatly contributes their persistence and success in colonizing skin mucosal areas humans animals. In an attempt discover cause resistance, we identified a gene, oatA , aureus . The corresponding deletion mutant had increased sensitivity lysozyme. HPLC electrospray ionization tandem mass spectrometry analyses cell wall revealed muramic acid peptidoglycan wild‐type strain was...
Growth of the mesh-like peptidoglycan (PG) sacculus located between bacterial inner and outer membranes (OM) is tightly regulated to ensure cellular integrity, maintain cell shape, orchestrate division. Cytoskeletal elements direct placement activity PG synthases from inside cell, but precise spatiotemporal control over this process poorly understood. We demonstrate that are also controlled outside sacculus. Two OM lipoproteins, LpoA LpoB, essential for function, respectively, PBP1A PBP1B,...
In Gram-negative bacteria, the outer membrane protects cell against many toxic molecules, and peptidoglycan layer provides protection osmotic challenges, allowing bacterial cells to survive in changing environments. Maintaining envelope integrity is therefore a question of life or death for cell. Here we show that Escherichia coli activate LD-transpeptidase LdtD introduce 3-3 cross-links when compromised, this response required avoid lysis. This remodeling program strategy increase overall...
Analytical work on the fractionation of glycan strands Streptococcus pneumoniae cell wall has led to observation that an unusually high proportion hexosamine units (over 80% glucosamine and 10% muramic acid residues) was not N-acetylated, explaining resistance peptidoglycan hydrolytic action lysozyme, a muramidase cleaves in backbone. A gene, pgdA, identified as encoding for peptidoglycanN-acetylglucosamine deacetylaseA with amino sequence similarity fungal chitin deacetylases rhizobial NodB...
Summary The tubulin homologue FtsZ is well known for its essential function in bacterial cell division. Here, we show that Caulobacter crescentus , also plays a major role elongation by spatially regulating the location of MurG, which produces lipid II peptidoglycan wall precursor. early assembly into highly mobile ring‐like structure during quickly followed recruitment MurG and redirection precursor synthesis to midcell region. These FtsZ‐dependent events occur before constriction...
ABSTRACT Streptococcus pneumoniae is one of the few species within group low-G +C gram-positive bacteria reported to contain no d -alanine in teichoic acids, although dltABCD operon encoding proteins responsible for -alanylation present genomes two S. strains, laboratory strain R6 and clinical isolate TIGR4. The annotation dltA predicts a protein, -alanine- -alanyl carrier protein ligase (Dcl), that shorter at amino terminus than all other Dcl proteins. Translation could also start upstream...
Summary The murein (peptidoglycan) sacculus is an essential polymer embedded in the bacterial envelope. Escherichia coli class B penicillin‐binding protein (PBP) 3 a transpeptidase and for cell division. In affinity chromatography experiment, bifunctional transglycosylase‐transpeptidase synthase PBP1B was retained by PBP3‐sepharose when membrane fraction of E. applied. direct protein–protein interaction between purified PBP3 characterized vitro surface plasmon resonance. confirmed vivo...
To maintain cellular structure and integrity during division, Gram-negative bacteria must carefully coordinate constriction of a tripartite cell envelope inner membrane, peptidoglycan (PG), outer membrane (OM). It has remained enigmatic how this is accomplished. Here, we show that machines facilitating septal PG synthesis (PBP1B-LpoB complex) OM (Tol system) are physically functionally coordinated via YbgF, renamed CpoB (Coordinator constriction, associated with PBP1B). localizes to the...