A5058214324- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- Protein Structure and Dynamics
- Pharmaceutical and Antibiotic Environmental Impacts
- Glycosylation and Glycoproteins Research
- Gene Regulatory Network Analysis
- Bone Tissue Engineering Materials
- Enzyme Production and Characterization
- Lipid Membrane Structure and Behavior
- Gut microbiota and health
- Microbial Metabolic Engineering and Bioproduction
- Cellular transport and secretion
- Legume Nitrogen Fixing Symbiosis
- Advancements in Transdermal Drug Delivery
- Polysaccharides and Plant Cell Walls
- Antimicrobial agents and applications
- Metabolomics and Mass Spectrometry Studies
- Surface Modification and Superhydrophobicity
Newcastle University
2010-2025
University of Birmingham
2018-2024
University of Nottingham
2023
European Molecular Biology Laboratory
2013-2021
National Institutes of Health
2020
Yale University
2020
Harvard University
2011-2020
Rockefeller University
2020
Brigham and Women's Hospital
2020
Federation of American Societies for Experimental Biology
2020
Growth of the mesh-like peptidoglycan (PG) sacculus located between bacterial inner and outer membranes (OM) is tightly regulated to ensure cellular integrity, maintain cell shape, orchestrate division. Cytoskeletal elements direct placement activity PG synthases from inside cell, but precise spatiotemporal control over this process poorly understood. We demonstrate that are also controlled outside sacculus. Two OM lipoproteins, LpoA LpoB, essential for function, respectively, PBP1A PBP1B,...
All bactericidal antibiotics were recently proposed to kill by inducing reactive oxygen species (ROS) production, causing destabilization of iron-sulfur (Fe-S) clusters and generating Fenton chemistry. We find that the ROS response is dispensable upon treatment with antibiotics. Furthermore, we demonstrate Fe-S are required for killing only aminoglycosides. In contrast cells, using major cluster biosynthesis machinery, ISC, cells alternative SUF, cannot efficiently mature respiratory...
Growth of the bacterial cell wall peptidoglycan sacculus requires co-ordinated activities synthases, hydrolases and morphogenesis proteins, but details these interactions are largely unknown. We now show that Escherichia coli glycosyltrasferase-transpeptidase PBP1A interacts with elongation-specific transpeptidase PBP2 in vitro cell. Cells lacking thinner initiate division later cycle. localizes mainly to cylindrical cell, supporting its role elongation. Our synthesis assays provide novel...
Mitophagy is a key process regulating mitochondrial quality control. Several mechanisms have been proposed to regulate mitophagy, but these mostly studied using stably expressed non-native proteins in immortalized cell lines. In skeletal muscle, mitophagy and its molecular require more thorough investigation. To measure directly, we generated stable muscle C2C12 line, expressing reporter construct (mCherry-green fluorescence protein-mtFIS1101-152). Here, report that both carbonyl cyanide...
The rod-shaped bacterium Escherichia coli grows by insertion of peptidoglycan into the lateral wall during cell elongation and synthesis new poles division. monofunctional transpeptidases PBP2 PBP3 are part specialized protein complexes called elongasome divisome, respectively, which catalyse extension maturation. Endogenous immunolabelled localized in cylindrical as well transiently at midcell. Using novel image analysis tool Coli-Inspector to analyse localization function bacterial age, we...
Article3 February 2020Open Access Outer membrane lipoprotein NlpI scaffolds peptidoglycan hydrolases within multi-enzyme complexes in Escherichia coli Manuel Banzhaf European Molecular Biology Laboratory, Genome Unit, Heidelberg, Germany Search for more papers by this author Hamish CL Yau Centre Bacterial Cell Biology, Biosciences Institute, Newcastle University, Upon Tyne, UK Jolanda Verheul & Physiology, Swammerdam Institute Life Sciences, Faculty of Science, University Amsterdam, The...
In this study, a new biodegradable alloy from the Zn-Ag-Zr system was investigated. Most importantly, mechanical properties and ductility were significantly improved in designed Zn1Ag0.05Zr comparison to binary Zn1Ag previously investigated Zn0.05Zr alloys (wt%). The characterized reached values of yield strength, ultimate tensile strength elongation failure equal 166 ± 2 MPa, 211 1 MPa 35 1%, respectively. Simultaneous addition both alloying elements contributed solid solution...
Abstract In vitro cytotoxicity assessment is indispensable in developing new biodegradable implant materials. Zn, which demonstrates an ideal corrosion rate between Mg‐ and Fe‐based alloys, has been reported to have excellent vivo biocompatibility . Therefore, modifications aimed at improving Zn's mechanical properties should not degrade its biological response. As sufficient strength, ductility behavior required of load‐bearing implants obtained plastically deformed Zn‐3Ag‐0.5Mg, the effect...
Current design strategies for biomedical tissue scaffolds are focused on multifunctionality to provide beneficial microenvironments support growth. We have developed a simple yet effective approach create core-shell fibers of poly(3-hydroxybuty-rate-co-3-hydroxyvalerate) (PHBV), which homogenously covered with titanium dioxide (TiO2) nanoparticles. Unlike the blend process, co-axial electrospinning enabled uniform distribution nanoparticles without formation large aggregates. observed 5...
Summary The distribution of PBP5, the major D,D‐carboxypeptidase in Escherichia coli , was mapped by immunolabelling and visualization GFP fusion proteins wild‐type cells mutants lacking one or more D,D‐carboxypeptidases. In addition to being scattered around lateral envelope, PBP5 also concentrated at nascent division sites prior visible constriction. Inhibiting PBP2 activity (which eliminates wall elongation) shifted midcell, whereas inhibiting PBP3 aborts divisome invagination) led...
The integrity of the cell envelope E. coli relies on concerted activity multi-protein machineries that synthesize peptidoglycan (PG) and outer membrane (OM). Our previous work found depletion lipopolysaccharide (LPS) export to OM induces an essential PG remodeling process involving LD-transpeptidases (LDTs), glycosyltransferase function PBP1B carboxypeptidase PBP6a. Consequently, cells with defective biogenesis lyse if they lack any these enzymes. Here we report morphological defects, lysis...
Objectives Staphylococcus aureus is a leading cause of hospital acquired infections worldwide. Over recent decades, methicillin resistant (MRSA), which to multiple antimicrobials, has emerged as significant pathogenic strain in both and community settings. The rapid emergence dissemination MRSA clones are driven by dynamic evolving population, spreading swiftly across regions on epidemiological time scales. Despite the vast geographical expanse diverse demographics Kingdom Saudi Arabia...
Salmonella enterica serovar Minnesota (S. Minnesota) is an emerging that persists within poultry supply chains, potentially causing outbreaks in humans. Understanding its population genomics crucial for designing preventive measures. We performed a genomic surveillance study of S. by analyzing 259 isolates from Saudi Arabia. Whole-genome sequencing data these were analyzed to characterize clones and the genetic factors underlying antimicrobial resistance virulence. compared all available...
Summary Bacteria surround their cytoplasmic membrane with an essential, stress‐bearing peptidoglycan (PG) layer consisting of glycan chains linked by short peptides into a mesh‐like structure. Growing and dividing cells expand PG using inner‐membrane anchored synthases, including Penicillin‐binding proteins (PBPs), which participate in dynamic protein complexes to facilitate cell wall growth. In Escherichia coli , presumably other Gram‐negative bacteria, growth the mainly single layered is...
The Gram-negative outer-membrane envelops the bacterium and functions as a permeability barrier against antibiotics, detergents, environmental stresses. Some virulence factors serve to maintain integrity of outer membrane, including DolP (formerly YraP) protein unresolved structure function. Here, we reveal is lipoprotein functionally conserved amongst bacteria that loss increases membrane fluidity. We present NMR solution for Escherichia coli DolP, which composed two BON domains form an...
The cell envelope is essential for viability in all domains of life. It retains enzymes and substrates within a confined space while providing protective barrier to the external environment. Destabilising bacterial pathogens common strategy employed by antimicrobial treatment. However, even one best studied organisms, Escherichia coli, there remain gaps our understanding how synthesis successive layers are coordinated during growth division. Here, we used whole-genome phenotypic screen...
Gram-negative bacteria have a thin peptidoglycan layer between the cytoplasmic and outer membranes protecting cell from osmotic challenges. Hydrolases of this structure are needed to cleave bonds allow newly synthesized strands be inserted by synthases. These enzymes need tightly regulated their activities coordinated prevent lysis. To better understand process in Escherichia coli, we probed genetic interactions mrcA (encodes PBP1A) mrcB PBP1B) with genes encoding amidases endopeptidases...
Nanomaterials of zinc oxide (ZnO) exhibit antibacterial activities under ambient illumination that result in cell membrane permeability and disorganization, representing an important opportunity for health-related applications. However, the development antibiofouling surfaces incorporating ZnO nanomaterials has remained limited. In this work, we fabricate superhydrophobic based on nanopillars. Water droplets these small contact angle hysteresis (within 2–3°) a minimal tilting 1°. Further,...