A5069035135- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- CRISPR and Genetic Engineering
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Pneumonia and Respiratory Infections
- Protein Degradation and Inhibitors
- Streptococcal Infections and Treatments
- Enzyme Structure and Function
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Antimicrobial Resistance in Staphylococcus
- Telomeres, Telomerase, and Senescence
The University of Queensland
2021-2024
Queensland University of Technology
2022
The University of Adelaide
2022
Queens University
2021
Sri Sathya Sai Institute of Higher Learning
2018
Quiescence, a reversible state of cell-cycle arrest, is an adaptive feature many adult tissue stem cells, including those in the brain. In gliomas, brain tumour cells that reside quiescent preferentially survive chemotherapy and radiotherapy, highlighting their critical role therapy resistance disease progression. To date, it remains unclear whether molecular programs governing these states are functionally conserved between neural cells. Here, we establish novel vitro models to study...
The cell envelope is essential for viability in all domains of life. It retains enzymes and substrates within a confined space while providing protective barrier to the external environment. Destabilising bacterial pathogens common strategy employed by antimicrobial treatment. However, even one best studied organisms, Escherichia coli, there remain gaps our understanding how synthesis successive layers are coordinated during growth division. Here, we used whole-genome phenotypic screen...
The study of clinically relevant bacterial pathogens relies on molecular and genetic approaches. However, the generally low transformation frequency among natural isolates poses technical hurdles to widely applying common methods in biology, including large constructs, chromosomal manipulation, dense mutant library construction. Here we demonstrate that culturing clinical presence polymyxin B nonapeptide (PMBN) improves their via electroporation by up 100-fold a dose-dependent reversible...
Gram-negative bacteria have a unique cell envelope with lipopolysaccharide-containing outer membrane that is tightly connected to thin layer of peptidoglycan. The tight connection between the and peptidoglycan needed maintain as an impermeable barrier for many toxic molecules antibiotics. Enterobacteriaceae such Escherichia coli covalently attach abundant membrane-anchored lipoprotein Lpp (Braun's lipoprotein) tripeptides in peptidoglycan, mediated by transpeptidases LdtA, LdtB, LdtC. LdtD...
Hydrogen peroxide (H2O2) is a common effector of defense mechanisms against pathogenic infections. However, bacterial factors involved in H2O2 tolerance remain unclear. Here we used transposon-directed insertion-site sequencing (TraDIS), technique allowing the screening whole genome, to identify genes implicated Escherichia coli. Our TraDIS analysis identified 10 mutants with fitness defect upon exposure, among which previously H2O2-associated (oxyR, dps, dksA, rpoS, hfq and polA) other no...
ABSTRACT Salmonella enterica is a globally disseminated pathogen that the cause of over 100 million infections per year. The resulting diseases are dependent upon host susceptibility and infecting serovar. As S. serovar Typhimurium induces typhoid-like disease in mice, this model has been used extensively to illuminate various aspects infection responses. Due severity model, researchers often use strains mice resistant or attenuated . Despite decades research, many fundamental biology remain...
Protein acylation is critical for many cellular functions across all domains of life. In bacteria, lipoproteins have important roles in virulence and are targets the development antimicrobials vaccines. Bacterial secreted from cytosol via Sec pathway acylated on an N-terminal cysteine residue through action three enzymes. Gram-negative Lol transports to outer membrane. Here, we demonstrate that Aat secretion system a composite sharing similarity with elements type I systems pathway. During...
ESBL-producing isolates of the Enterobacteriaceae occur throughout world. The objectives this study were to characterize uropathogenic Escherichia coli isolated at a tertiary care hospital in southern India, and shed light on blaCTX-M sequences Indian origin. A cohort 13 urinary E. (obtained from patients Sri Sathya Sai Institute Higher Medical Sciences, Prasanthigram, Andhra Pradesh, India) characterized found be resistant multiple antibiotics, including extended-spectrum cephalosporins....
Abstract Small or overlapping genes are prevalent across all domains of life but often overlooked for annotation and function because challenges in their detection. The advent high-density mutagenesis data-mining studies suggest the existence further coding potential within bacterial genomes. To overcome limitations existing protein detection methods, we applied a genetics-based approach. We combined transposon insertion sequencing with translation reporter to identify translated open...
ABSTRACT Gram-negative bacteria have a unique cell envelope with lipopolysaccharide-containing outer membrane that is tightly connected to thin layer of peptidoglycan. The tight connection between the and peptidoglycan needed maintain as an impermeable barrier for many toxic molecules antibiotics. Enterobacteriaceae such Escherichia coli covalently attach abundant membrane-anchored lipoprotein Lpp (Braun’s lipoprotein) tripeptides in peptidoglycan, mediated by transpeptidases LdtA, LdtB...
Abstract The cell envelope is essential for viability in all kingdoms of life. It retains enzymes and substrates within a confined space while providing protective barrier to the external environment. Destabilising bacterial pathogens common strategy employed by antimicrobial treatment. However, even one most well studied organisms, Escherichia coli , there remain gaps our understanding how synthesis successive layers are coordinated during growth division. Here, we used whole genome...
Abstract Salmonella enterica is a globally disseminated pathogen that the cause of over 100 million infections per year. The resulting diseases caused by S. are dependent upon host susceptibility and infecting serovar. For example, Typhoid fever human exclusive disease serovar Typhi. As Typhimurium induces typhoid like in mice, this model has been used extensively to illuminate various aspects infection responses. However, so severe even one infectious bacterium injected intravenously will...
Abstract The study of clinically relevant bacterial pathogens relies on molecular and genetic approaches. However, the generally low transformation frequency among natural isolates poses technical hurdles to widely applying common methods in biology, including large constructs, chromosomal manipulation, dense mutant library construction. Here we demonstrate that culturing clinical presence polymyxin B nonapeptide (PMBN) improves their via electroporation by up 100-fold a dose-dependent...