A5063498043- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Glycosylation and Glycoproteins Research
- Trace Elements in Health
- Extraction and Separation Processes
- Antimicrobial Resistance in Staphylococcus
- Recycling and Waste Management Techniques
- CRISPR and Genetic Engineering
- Biochemical Acid Research Studies
- Insect and Pesticide Research
- Antimicrobial agents and applications
- Prion Diseases and Protein Misfolding
- Yersinia bacterium, plague, ectoparasites research
- SARS-CoV-2 and COVID-19 Research
- Medicinal Plant Research
- Enterobacteriaceae and Cronobacter Research
- Chromium effects and bioremediation
Queensland University of Technology
2019-2025
The University of Sydney
2012-2025
James Cook University
2025
University of Science and Technology of China
2024
University of Illinois Urbana-Champaign
2017-2023
Anshan Hospital
2019
Korea University
2007
Abstract The NASA Cloud, Aerosol, and Monsoon Processes Philippines Experiment (CAMP 2 Ex) employed the P-3, Stratton Park Engineering Company (SPEC) Learjet 35, a host of satellites surface sensors to characterize coupling aerosol processes, cloud physics, atmospheric radiation within Maritime Continent’s complex southwest monsoonal environment. Conducted in late summer 2019 from Luzon, Philippines, conjunction with Office Naval Research Propagation Intraseasonal Tropical Oscillations...
Most Gram-negative bacteria synthesize a plethora of cell surface polysaccharides that play key roles in immune evasion, envelope structural integrity and host-pathogen interactions. In the predominant polysaccharide Wzx/Wzy-dependent pathway, synthesis is divided between cytoplasmic periplasmic faces membrane. Initially, an oligosaccharide composed 3-8 sugars synthesized on membrane-embedded lipid carrier, undecaprenyl pyrophosphate, within face This lipid-linked then translocated to by Wzx...
The most common system for synthesis of cell surface polysaccharides is the Wzx/Wzy-dependent pathway, which involves synthesis, on cytoplasmic face membrane, repeat units, are then translocated to periplasmic by a Wzx translocase and polymerized Wzy generate polysaccharide. One such polysaccharide O antigen, incorporated into lipopolysaccharide (LPS). antigen extremely variable, with over 186 forms in Escherichia coli. proteins also very diverse, but they have been thought be specific only...
Summary This work was designed to investigate the phenolic composition, antioxidant and pancreatic lipase inhibitory activities of Rhus chinensis Mill. fruits extracted by different solvents, illustrate which major compounds were responsible for inhibition evaluate their interactions. Results showed that R. rich in phenolics, included 13 types identified quantified UHPLC ‐ ESI HRMS / MS . Among myricetin‐3‐ O ‐rhamnoside quercetin‐3‐ most dominant detected all extracts. Extracts with 80%...
Most Gram-negative bacteria have an O antigen, a polysaccharide with many repeats of short oligosaccharide that is part the lipopolysaccharide, major lipid in outer leaflet membrane. Lipopolysaccharide variable 46 forms Salmonella enterica underpin serotyping scheme. Repeat units are assembled on carrier embedded cell membrane, and then translocated by Wzx translocase from cytoplasmic face to followed polymerization. The antigen incorporated into lipopolysaccharide exported widely thought be...
Bacterial polysaccharide synthesis is catalysed on the universal lipid carrier, undecaprenol phosphate (UndP). The cellular UndP pool shared by different pathways including peptidoglycan biogenesis. Disruptions in cytosolic steps are detrimental to bacterial survival due effects recycling. In contrast, bacteria can survive disruptions periplasmic steps, suggesting a tolerance mechanism mitigate sequestration. Here we investigated mechanisms of polymerases that involved UndP-releasing two...
Changes in membrane composition govern the fitness and survival of bacteria response to environmental stresses. One critical parameter is fluidity, which dictates overall integrity, adaptability fluctuations (e.g., temperature changes), functionality interactions between integral peripheral proteins. In recent years, substantial evidence has shown that fluidity finely tuned for biofilm formation. While high promotes robust formation Klebsiella pneumoniae, this challenged by reports multiple...
Bile salts (BS) are derived from cholesterol in the liver and act as antimicrobial agents intestines by disrupting bacterial cell membranes inducing oxidative stress. The gut bacterium E. coli is naturally resistant to BS, including model strain K12 that produces a truncated LPS without O-antigen (OAg). Paradoxically, restoring wild-type like with OAg sensitises exogenous BS. In this study, we investigate phenomenon. We show mutations causing truncation of core oligosaccharide render these...
Biofilm dispersal agents, like nitric oxide (NO), restore antimicrobial effectiveness against biofilm infections by inducing bacteria to shift from a planktonic state, thereby overcoming the tolerance typically associated with biofilms. Sodium nitroprusside (SNP) is widely used NO donor for investigating molecular mechanisms underlying NO-mediated in nosocomial pathogen Pseudomonas aeruginosa . However, effects of SNP are variable depending on vitro experimental conditions, some studies...
ABSTRACT The O antigen (OAg) polysaccharide is one of the most diverse surface molecules Gram-negative bacterial pathogens. structural classification OAg, based on serological typing and sequence analysis, important in epidemiology surveillance outbreaks infections. Despite chemical structures OAg repeating units (RUs), genetic basis RU assembly remains poorly understood represents a major limitation assigning gene functions biosynthesis. Here, we describe approach to interrogate functional...
The study of clinically relevant bacterial pathogens relies on molecular and genetic approaches. However, the generally low transformation frequency among natural isolates poses technical hurdles to widely applying common methods in biology, including large constructs, chromosomal manipulation, dense mutant library construction. Here we demonstrate that culturing clinical presence polymyxin B nonapeptide (PMBN) improves their via electroporation by up 100-fold a dose-dependent reversible...
The Wzx/Wzy-dependent pathway is the predominant for O-antigen production in Gram-negative bacteria. repeat unit (O unit) an oligosaccharide that assembled at cytoplasmic face of membrane on undecaprenyl pyrophosphate. Wzx then flips it to periplasmic polymerization by Wzy, which adds O reducing end a growing O-unit polymer each round polymerization. and Wzy both exhibit enormous sequence diversity. It has recently been shown that, contrary earlier reports, efficiency diverse forms can be...
Bacterial Wzx flippases translocate (flip) short oligosaccharide repeat units (O units) across the inner membrane into periplasm, which is a critical step in assembly of many O antigens, capsules and other surface polysaccharides. There enormous diversity antigens particular, even within species. proteins are similarly diverse, but it has been widely accepted that they have significant specificity only for first sugar an unit. In this study, we analysed from Salmonella enterica group C2...
The Wzx/Wzy O-antigen pathway involves synthesis of a repeat unit (O unit) consisting 3 to 8 sugars on an inner-membrane-embedded lipid carrier. These O units are translocated across the membrane its periplasmic face by Wzx, while retaining linkage carrier, and then polymerized Wzy polymer, which WaaL ligase transfers lipopolysaccharide precursor complete synthesis, concomitantly releasing This carrier is also used for peptidoglycan assembly, sequestration known be toxic. Thus, O-unit must...
Lipoic acid is synthesized by a remarkably atypical pathway in which the cofactor assembled on its cognate proteins. An octanoyl moiety diverted from fatty synthesis covalently attached to acceptor protein, and sulfur insertion at carbons 6 8 of form lipoyl cofactor. Covalent attachment this required for function several central metabolism enzymes, including glycine cleavage H protein (GcvH). In Bacillus subtilis, GcvH sole substrate lipoate assembly. Hence lipoic acid-requiring 2-oxoacid...
Escherichia coli K-12 is a model organism for bacteriology and has served as workhorse molecular biology biochemistry over century since its first isolation in 1922. However, strains are phenotypically devoid of an O antigen (OAg) early reports the scientific literature. Recent studies have reported presence independent mutations that abolish OAg repeating-unit (RU) biogenesis E. from same original source, suggesting unknown evolutionary forces selected inactivation during propagation K-12....
Antibiotic resistance is one of the most prominent threats to modern medicine. In latest World Health Organization list bacterial pathogens that urgently require new antibiotics, 9 out 12 are Gram-negative, with four being "critical priority." One crucial barrier restricting antibiotic efficacy against Gram-negative bacteria their unique cell envelope. While fatty acids a shared constituent all structural membrane lipids, biosynthesis pathway in distinct from eukaryotes, making it an...
Abstract Antibiotics are failing fast, and the development pipeline remains alarmingly dry. New drug research is being urged by world health officials, with new antibacterials against multidrug-resistant Gram-negative pathogens as highest priority. Antivirulence drugs, which inhibit bacterial pathogenicity factors, a class of promising antibacterials, however, their stifled lack standardised preclinical testing akin to what guides antibiotic development. The established target-specific...
O antigens are polysaccharides consisting of repeat units three to eight sugars, generally assembled by genes in a discrete antigen gene cluster. Salmonella enterica produces 46 forms antigen, and most the variation is determined However some cases structures modified enzymes encoded outside cluster, several such modifications have been reported for group E, with on bacteriophages one at distant chromosomal site. We identified enzyme, WbaK, that responsible O-acetylating subgroup E1 found...
By targeting the essential viral RNA-dependent RNA polymerase (RdRP), nucleoside analogs (NAs) have exhibited great potential in antiviral therapy for virus-related diseases. However, most ribose-modified NAs do not present broad-spectrum features, likely due to differences ribose-RdRP interactions across virus families. Here, we show that HNC-1664, an adenosine analog with modifications both ribose and base, has activity against positive-strand coronaviruses negative-strand arenaviruses....
Aims: Thioredoxin (TRX)-fold proteins are ubiquitous in nature. This redox scaffold has evolved to enable a variety of functions, including regulation, protein folding, and oxidative stress defense. In bacteria, the TRX-like disulfide bond (Dsb) family mediates folding multiple required for fitness pathogenic potential. Conventionally, Dsb have specific functions with monomeric dimeric Dsbs exclusively catalyzing thiol oxidation isomerization, respectively. contrasts eukaryotic forming...