A5019644976- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Urinary Tract Infections Management
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
- Endoplasmic Reticulum Stress and Disease
- Urinary Bladder and Prostate Research
- Protein Structure and Dynamics
- Antimicrobial Peptides and Activities
- Oral microbiology and periodontitis research
- Enterobacteriaceae and Cronobacter Research
- Porphyrin Metabolism and Disorders
- Pelvic floor disorders treatments
- Bladder and Urothelial Cancer Treatments
- Antimicrobial agents and applications
- Enzyme Structure and Function
- Probiotics and Fermented Foods
- Microbial Natural Products and Biosynthesis
- Viral gastroenteritis research and epidemiology
- Pediatric Urology and Nephrology Studies
- RNA and protein synthesis mechanisms
Queensland University of Technology
2016-2025
Queens University
2022
The University of Queensland
2009-2019
National Institute of Biomedical Innovation, Health and Nutrition
2019
Brisbane School of Theology
2019
University of Edinburgh
2006-2008
Moredun Research Institute
2005
Escherichia coli sequence type 131 (ST131) is a globally disseminated, multidrug resistant (MDR) clone responsible for high proportion of urinary tract and bloodstream infections. The rapid emergence successful spread E. ST131 strongly associated with several factors, including resistance to fluoroquinolones, virulence gene content, the possession 1 fimbriae FimH30 allele, production CTX-M-15 extended spectrum β-lactamase (ESBL). Here, we used genome sequencing examine molecular epidemiology...
Escherichia coli strains causing urinary tract infection (UTI) are increasingly recognized as belonging to specific clones. E. clone O25b:H4-ST131 has recently emerged globally a leading multi-drug resistant pathogen and bloodstream infections in hospitals the community. While most molecular studies date examine mechanisms conferring resistance ST131, relatively little is known about their virulence potential. Here we examined ST131 clinical isolates from two geographically diverse...
Escherichia coli ST131 is a globally disseminated, multidrug resistant clone responsible for high proportion of urinary tract and bloodstream infections. The rapid emergence successful spread E. strongly associated with antibiotic resistance; however, this phenotype alone unlikely to explain its dominance amongst uropathogens circulating worldwide in hospitals the community. Thus, greater understanding molecular mechanisms that underpin fitness required. In study, we employed hyper-saturated...
Chaperone-usher (CU) fimbriae are adhesive surface organelles common to many Gram-negative bacteria. Escherichia coli genomes contain a large variety of characterised and putative CU fimbrial operons, however, the classification annotation individual loci remains problematic. Here we describe model based on usher phylogeny genomic locus position categorise types E. coli. Using BLASTp algorithm, an iterative protein search was performed identify operons from 35 (and one fergusonnii)...
The ability of Escherichia coli to colonize both intestinal and extraintestinal sites is driven by the presence specific virulence factors, among which are autotransporter (AT) proteins. Members trimeric AT adhesin family important factors for several gram-negative pathogens mediate adherence eukaryotic cells extracellular matrix (ECM) In this study, we characterized a new (UpaG) from uropathogenic E. (UPEC). Molecular analysis UpaG revealed that it translocated cell surface adopts...
Escherichia coli ST131 is now recognised as a leading contributor to urinary tract and bloodstream infections in both community clinical settings. Here we present the complete, annotated genome of E. EC958, which was isolated from urine patient presenting with infection Northwest region England represents most well characterised strain. Sequencing carried out using Pacific Biosciences platform, provided sufficient depth read-length produce complete without need for other technologies. The...
Significance Many persistent and chronic bacterial infections are associated with the formation of large cell aggregates biofilms that difficult to treat. This includes respiratory urinary tract infections, on medical devices, ear, gums, heart. One mechanism used by bacteria aggregate form involves expression self-associating surface-located autotransporter proteins such as Antigen 43 (Ag43). Here we present crystal structure functional passenger domain Ag43 demonstrate its unique L-shaped...
Background. Escherichia coli O25b:H4-ST131 represents a predominant clone of multidrug-resistant uropathogens currently circulating worldwide in hospitals and the community. Urinary tract infections (UTIs) caused by E. ST131 are typically associated with limited treatment options often recurrent. Methods. Using established mouse models acute chronic UTI, we mapped pathogenic trajectory reference UTI isolate, strain EC958. Results. We demonstrated that EC958 can invade bladder epithelial...
Urinary tract infection (UTI) is among the most common infectious diseases of humans and nosocomial in developed world. They cause significant morbidity mortality, with approximately 150 million cases globally per year. It estimated that 40-50% women 5% men will develop a UTI their lifetime, accounts for more than 1 hospitalizations $1.6 billion medical expenses each year USA. Uropathogenic E. coli (UPEC) primary UTI. This review presents an overview virulence factors UPEC, major host...
Urinary tract infection (UTI) is one of the most common bacterial infections in humans, with uropathogenic Escherichia coli (UPEC) leading causative organism. UPEC has a number virulence factors that enable it to overcome host defenses within urinary and establish infection. The O antigen capsular polysaccharide are two such provide survival advantage UPEC. Here we describe application rpsL counter selection system construct capsule (kpsD) (waaL) mutants complemented derivatives three...
Abstract Saliva has attracted attention as a diagnostic fluid due to the association of oral microbiota with systemic diseases. However, lack standardised methods for saliva collection led slow uptake in microbiome research. The aim this study was systematically evaluate potential effects on salivary profiles using different collection, storage and gDNA extraction. Three types fractions were collected from healthy individuals or without stabilising buffer. Subsequently, three extraction...
The oral microbiome can play a role in the instigation and progression of diseases that manifest into other systemic conditions. These associations encourage exploration dysbiosis leading to pathogenesis cancers. In this study, rinse was used characterize fluctuation associated with cavity cancer (OCC) oropharyngeal cancers (OPC). study cohort consists normal healthy controls (n = 10, between 20 30 years age; n above 50 age), high-risk individuals 11, age bad hygiene and/or diseases) OCC OPC...
ABSTRACT The molecular mechanisms that define asymptomatic bacteriuria (ABU) Escherichia coli colonization of the human urinary tract remain to be properly elucidated. Here, we utilize ABU E. strain 83972 as a model dissect contribution siderophores iron acquisition, growth, fitness, and tract. We show produces enterobactin, salmochelin, aerobactin, yersiniabactin examine role these systems using mutants defective in siderophore biosynthesis uptake. Enterobactin aerobactin contributed most...
Early transcriptional activation events that occur in bladder immediately following bacterial urinary tract infection (UTI) are not well defined. In this study, we describe the whole transcriptome of uropathogenic Escherichia coli (UPEC) cystitis mice using genome-wide expression profiling to define innate immune stemming from UPEC colonization bladder. Bladder RNA female C57BL/6 mice, analyzed 1.0 ST-Affymetrix microarrays, revealed extensive diverse sets response genes, including those...
ABSTRACT Escherichia coli is the primary cause of urinary tract infection (UTI) in developed world. The major factors associated with virulence uropathogenic E. (UPEC) are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate responses. Another group adhesins represented by autotransporter (AT) subgroup proteins. In this study, we identified a new AT-encoding gene, termed upaH , present 6.5-kb unannotated intergenic region genome prototypic UPEC strain...