A5029597544- Gene expression and cancer classification
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA modifications and cancer
- Bioinformatics and Genomic Networks
- Epigenetics and DNA Methylation
- Genetic and Kidney Cyst Diseases
- Genetic Mapping and Diversity in Plants and Animals
- MicroRNA in disease regulation
- Genetic factors in colorectal cancer
- Gut microbiota and health
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Renal and related cancers
- Cancer-related molecular mechanisms research
- Gene Regulatory Network Analysis
- Muscle Physiology and Disorders
- Urinary Tract Infections Management
- RNA Research and Splicing
- Genetic Syndromes and Imprinting
- Mitochondrial Function and Pathology
- Congenital heart defects research
- Cancer-related gene regulation
- Advanced Biosensing Techniques and Applications
- Long-Term Effects of COVID-19
- Diabetes Treatment and Management
Emory University
2018-2025
Atlanta VA Medical Center
2021-2025
Hebei Agricultural University
2024-2025
Atlanta VA Health Care System
2020-2025
United States Department of Veterans Affairs
2024
Veterans Health Administration
2021-2024
Rollins College
2024
Shanxi Datong University
2024
Guangzhou University of Chinese Medicine
2021
Southern Medical University
2021
Combining information across genes in the statistical analysis of microarray data is desirable because relatively small number points obtained for each individual gene. Here we develop an estimator error variance that can borrow using James-Stein shrinkage concept. A new test statistic (FS) constructed this estimator. The compared with other statistics used to differential expression: gene-specific F (F1), pooled-variance (F3), a hybrid (F2) uses average and pooled variances, regularized...
The T cytoplasm of maize serves as a model for the nuclear restoration cytoplasmic male sterility. rf2 gene, one two genes required fertility in male-sterile T-cytoplasm (cmsT) maize, was cloned. protein predicted by sequence is putative aldehyde dehydrogenase, which suggests several mechanisms that might explain Rf2-mediated cmsT maize. Aldehyde dehydrogenase may be involved detoxification acetaldehyde produced ethanolic fermentation during pollen development, play role energy metabolism,...
Abstract Introduction Prior studies have established altered microbiota and immunologic reactivity to enteric commensal organisms in inflammatory bowel disease (IBD). Since intestinal inflammation is present a subset of patients with both pediatric adult spondyloarthritis (SpA), we hypothesized that SpA may also immune responsiveness organisms. Methods Stool blood specimens were collected from children enthesitis-related arthritis (ERA) non-inflammatory controls. DNA purified stool was...
Some plant cytoplasms express novel mitochondrial genes that cause male sterility. Nuclear disrupt the accumulation of corresponding gene products can restore fertility to such plants. The Texas (T) cytoplasm genome maize expresses a protein, URF13, which is necessary for T cytoplasm–induced Working in concert, functional alleles two nuclear genes, rf1 and rf2, Rf1 alleles, but not Rf2 reduce URF13. Hence, differs from typical restorers it does alter protein This study established rf2...
Recent advances in next-generation sequencing (NGS) technology enable researchers to collect a large volume of metagenomic data. These data provide valuable resources for investigating interactions between the microbiome and host environmental/clinical factors. In addition well-known properties count measurements, example, varied total sequence reads across samples, over-dispersion zero-inflation, studies usually samples with hierarchical structures, which introduce correlation among thus...
Previous reports have implicated an induction of genes in IFN/STAT1 (Interferon/STAT1) signaling radiation resistant and prosurvival tumor phenotypes a number cancer cell lines, we hypothesized that upregulation these may be predictive poor survival outcome and/or treatment response Glioblastoma Multiforme (GBM) patients. We developed list 8 related to hypothesize GBM Our working hypothesis over-expression this gene signature predicts patients was confirmed, addition, it demonstrated the...
Using genomic microarray analysis, we sought to identify and annotate differences in the pretraining skeletal muscle transcriptomes among human subjects clustered as nonresponders (Non), modest responders (Mod), extreme (Xtr) based on differential magnitudes of myofiber hypertrophy response progressive resistance training (RT) (Non-6 μm², Mod 1,111 or Xtr 2,475 μm²). In prior work, noted clusters prevalence myogenic stem cells during RT (35), translational signaling responses first bout...
Across numerous model systems, aging skeletal muscle demonstrates an impaired regenerative response when exposed to the same stimulus as young muscle. To better understand impact of in a human model, we compared changes transcriptome induced by unaccustomed high-intensity resistance loading (RL) sufficient cause moderate damage (37 yr) vs. older (73 adults. Serum creatine kinase was elevated 46% 24 h after RL all subjects with no age differences, indicating similar degrees myofiber membrane...
Early transcriptional activation events that occur in bladder immediately following bacterial urinary tract infection (UTI) are not well defined. In this study, we describe the whole transcriptome of uropathogenic Escherichia coli (UPEC) cystitis mice using genome-wide expression profiling to define innate immune stemming from UPEC colonization bladder. Bladder RNA female C57BL/6 mice, analyzed 1.0 ST-Affymetrix microarrays, revealed extensive diverse sets response genes, including those...
The study of metabolism has had a long history. Metabolomics, systems biology discipline representing analysis known and unknown pathways metabolism, grown tremendously over the past 20 years. Because its comprehensive nature, metabolomics requires careful consideration question(s) being asked, scale needed to answer question(s), collection storage sample specimens, methods for extraction metabolites from biological matrices, analytical method(s) be employed quality control analyses, how...
Metabolomics is perhaps the most challenging of -omics fields, given complexity an organism's metabolome and rapid rate at which it changes. When one sets out to study metabolism there are numerous dynamic variables that can influence must be considered. Recognizing experimental challenges confronting researchers who undertake studies, workshops like University Alabama Birmingham have been established offer instructional guidance. A summary UAB course training materials being published as a...
Two channel microarray data often contain systematic variations that can be minimized by transformation prior to further analysis. The most commonly observed effects are revealed viewing scatter plots of the logarithm ratio average logarithmic intensity two color channels (RI plots). In this paper we present a general model for signal with multiple error sources. We demonstrate how these sources influence shape an RI plot. then compare some currently available strategies in terms their...
Left ventricular (LV) volume overload (VO) causes eccentric remodeling with inflammatory cell infiltration and extracellular matrix (ECM) degradation, for which there is currently no proven therapy. To uncover new pathways that connect inflammation ECM homeostasis cellular dysfunction, we determined the cardiac transciptome in subacute, compensated, decompensated stages based on vivo hemodynamics echocardiography rat aortocaval fistula (ACF). LV dilatation at 5 wk was associated a normal...
Metabolomics, a systems biology discipline representing analysis of known and unknown pathways metabolism, has grown tremendously over the past 20 years. Because its comprehensive nature, metabolomics requires careful consideration question(s) being asked, scale needed to answer question(s), collection storage sample specimens, methods for extraction metabolites from biological matrices, analytical method(s) be employed quality control analyses, how collected data are correlated, statistical...
DNA methylation, a major epigenetic mechanism, may regulate coordinated expression of multiple genes at specific time points during alveolar septation in lung development. The objective this study was to identify regulated by methylation normal mice and disordered bronchopulmonary dysplasia. In mice, newborn lungs (preseptation) adult (postseptation) were evaluated microarray analysis gene immunoprecipitation methylated followed sequencing (MeDIP-Seq). humans, data integrated with...