A5004968684- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- ATP Synthase and ATPases Research
- Bacterial biofilms and quorum sensing
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- Machine Learning in Bioinformatics
- Vibrio bacteria research studies
- Metabolism and Genetic Disorders
- Fungal and yeast genetics research
- Protein Structure and Dynamics
- Escherichia coli research studies
- Heat shock proteins research
- Bacterial Identification and Susceptibility Testing
- Enzyme Structure and Function
- Plant Virus Research Studies
- Ubiquitin and proteasome pathways
- Streptococcal Infections and Treatments
- Parasitic Infections and Diagnostics
- Endoplasmic Reticulum Stress and Disease
- Cancer Research and Treatments
Australian Regenerative Medicine Institute
2016-2025
Monash University
2016-2025
Discovery Institute
2015-2024
Impact
2022-2024
The University of Queensland
2024
Czech Academy of Sciences, Institute of Microbiology
2022
Institute of Infection and Immunity
2020
The University of Melbourne
2001-2017
Jouf University
2017
Mayo Hospital
2017
Bacterial viruses are among the most numerous biological entities within human body. These found regions of body that have conventionally been considered sterile, including blood, lymph, and organs. However, primary mechanism bacterial use to bypass epithelial cell layers access remains unknown. Here, we used in vitro studies demonstrate rapid directional transcytosis diverse bacteriophages across confluent originating from gut, lung, liver, kidney, brain. Bacteriophage had a significant...
Klebsiella pneumoniae causes significant morbidity and mortality worldwide, particularly amongst hospitalized individuals. The principle mechanism for pathogenesis in hospital environments involves the formation of biofilms, primarily on implanted medical devices. In this study, we constructed a transposon mutant library clinical isolate, K. AJ218, to identify genes pathways implicated biofilm formation. Three mutants severely defective contained insertions within mrkABCDF encoding main...
Mitochondria fulfill central functions in cellular energetics, metabolism, and signaling. The outer membrane translocator complex (the TOM complex) imports most mitochondrial proteins, but its architecture is unknown. Using a cross-linking approach, we mapped the active down to single amino acid residues, revealing different transport paths for preproteins through Tom40 channel. An N-terminal segment of passes from cytosol channel recruit chaperones intermembrane space that guide transfer...
Evolutionary information in the form of a Position-Specific Scoring Matrix (PSSM) is widely used and highly informative representation protein sequences. Accordingly, PSSM-based feature descriptors have been successfully applied to improve performance various predictors attributes. Even though number algorithms proposed previous studies, there currently no universal web server or toolkit available for generating this wide variety descriptors. Here, we present POSSUM ( Po sition- S pecific...
Integral proteins in the outer membrane of mitochondria control all aspects organelle biogenesis, being required for protein import, mitochondrial fission, and, metazoans, programmed cell death. How these integral are assembled had been unclear. In bacteria, Omp85 is an essential component insertion machinery, and we show that members family also found eukaryotes ranging from plants to humans. eukaryotes, present membrane. The gene encoding viability yeast, conditional omp85 mutants have...
The yeast cytosol contains multiple homologs of the DnaK and DnaJ chaperone family. Our current understanding which functionally interact is incomplete. Zuotin a homolog bound to ribosome. We have now identified Ssz1p/Pdr13p as zuotin's partner chaperone. Ssz1p form ribosome-associated complex (RAC) that ribosome via zuotin subunit. RAC unique among eukaryotic DnaK-DnaJ systems, 1:1 stable, even in presence ATP or ADP. In vitro , stimulates translocation ribosome-bound mitochondrial...
Inhibitors of apoptosis (IAPs) are a family proteins that bear baculoviral IAP repeats (BIRs) and regulate in vertebrates Drosophila melanogaster . The yeasts Saccharomyces cerevisiae Schizosaccharomyces pombe both encode single IAP, designated BIR1 bir1 , respectively, each which bears two BIRs. In rich medium, mutant S. underwent normal vegetative growth mitosis. Under starvation conditions, however, diploids formed spores inefficiently, instead undergoing pseudohyphal differentiation....
A class of integral membrane proteins, referred to as ‘tail‐anchored proteins’, are inserted into phospholipid bilayers via a single segment hydrophobic amino acids at the C‐terminus, thereby displaying large functional domain in cytosol. This attachment strategy allows eukaryotic cells position wide range cytoplasmic activities close surface an intracellular membrane. Tail‐anchored proteins often, but not always, demonstrate selective distribution specific organelles. membrane‐specific is...