A5027644421- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Immune Cell Function and Interaction
- Bacterial Genetics and Biotechnology
- Immunotherapy and Immune Responses
- Bacterial biofilms and quorum sensing
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Genomics and Phylogenetic Studies
- Helicobacter pylori-related gastroenterology studies
- Reproductive tract infections research
- Syphilis Diagnosis and Treatment
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Microbial infections and disease research
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- Virology and Viral Diseases
- RNA and protein synthesis mechanisms
- Enterobacteriaceae and Cronobacter Research
- Influenza Virus Research Studies
Peter Doherty Institute
2016-2025
The University of Melbourne
2016-2025
In-Q-Tel
2021
The Royal Melbourne Hospital
1995-2020
Monash University
1984-2017
Jouf University
2017
Wellcome Sanger Institute
2017
Mayo Hospital
2017
Government College University, Lahore
2017
The University of Queensland
2016
Klebsiella pneumoniae is now recognized as an urgent threat to human health because of the emergence multidrug-resistant strains associated with hospital outbreaks and hypervirulent severe community-acquired infections. K. ubiquitous in environment can colonize infect both plants animals. However, little known about population structure pneumoniae, so it difficult recognize or understand clinically important clones within this highly genetically diverse species. Here we present a detailed...
Klebsiella pneumoniae is an opportunistic pathogen and leading cause of hospital-associated infections. Intensive care unit (ICU) patients are particularly at risk. part the healthy human microbiome, providing a potential reservoir for infection. However, frequency gut colonization its contribution to infections not well characterized. We conducted 1-year prospective cohort study in which 498 ICU were screened rectal throat carriage K. shortly after admission. isolated from screening swabs...
Many enteropathogenic bacteria target the mammalian gut. The mechanisms protecting host from infection are poorly understood. We have studied protective functions of secretory antibodies (sIgA) and microbiota, using a mouse model for S. typhimurium diarrhea. This pathogen is common cause diarrhea in humans world-wide. (S. tmatt, sseD) causes self-limiting gut streptomycin-treated mice. After 40 days, all animals had overcome disease, developed sIgA response, most cleared lumen. limited...
Klebsiella pneumoniae causes significant morbidity and mortality worldwide, particularly amongst hospitalized individuals. The principle mechanism for pathogenesis in hospital environments involves the formation of biofilms, primarily on implanted medical devices. In this study, we constructed a transposon mutant library clinical isolate, K. AJ218, to identify genes pathways implicated biofilm formation. Three mutants severely defective contained insertions within mrkABCDF encoding main...
Mucosal associated invariant T (MAIT) cells recognise conserved microbial metabolites from riboflavin synthesis. Striking evolutionary conservation and pulmonary abundance implicate them in antibacterial host defence, yet their functions protection against clinically important pathogens are unknown. Here we show that mouse Legionella longbeachae infection induces MR1-dependent MAIT cell activation rapid accumulation of with immune detectable immunocompetent animals. is more evident mice...
Abstract Klebsiella pneumoniae is a major cause of opportunistic healthcare-associated infections, which are increasingly complicated by the presence extended-spectrum beta-lactamases (ESBLs) and carbapenem resistance. We conducted year-long prospective surveillance study K. clinical isolates in hospital patients. Whole-genome sequence (WGS) data reveals diverse pathogen population, including other species within complex (18%). Several infections were caused variicola/K. hybrids, one shows...
The production of IgA is induced in an antigen-unspecific manner by commensal flora. These secretory antibodies (SAbs) may bind multiple antigens and are thought to eliminate bacteria self-antigens avoid systemic recognition. In this study, we addressed the role “innate” SAbs, i.e., those that continuously produced normal individuals, protection against infection gastrointestinal tract. We used polymeric immunoglobulin receptor (pIgR−/−) knock-out mice, which unable actively transport...
Streptococcus pneumoniae (the pneumococcus) kills ~1.6 million people annually. Pneumococcal infections predominantly manifest as pneumonia, sepsis, meningitis, and otitis media. S. is also a member of the normal nasopharyngeal flora, colonizing up to 80% children. Infection with influenza A virus (IAV) has been associated both pneumococcal disease transmission. However, date no animal model available investigate role IAV in spread pneumoniae. Here we pneumococcal-influenza synergism...
Abstract RNA sequencing studies have identified hundreds of non‐coding s in bacteria, including regulatory small ( sRNA ). However, our understanding function has lagged behind their identification due to a lack tools for the high‐throughput analysis – interactions bacteria. Here we demonstrate that vivo mRNA duplexes can be recovered using UV ‐crosslinking, ligation and hybrids CLASH Many recruit endoribonuclease, RN ase E, facilitate processing s. We were able recover base‐paired...
Programmed cell death contributes to host defense against pathogens. To investigate the relative importance of pyroptosis, necroptosis, and apoptosis during Salmonella infection, we infected mice macrophages deficient for diverse combinations caspases-1, -11, -12, -8 receptor interacting serine/threonine kinase 3 (RIPK3). Loss caspase-8-driven apoptosis, or necroptosis had minor impact on control. However, combined deficiency these pathways caused loss bacterial control in their macrophages,...
Klebsiella pneumoniae is a leading cause of extended-spectrum β-lactamase (ESBL)-producing hospital-associated infections, for which elderly patients are at increased risk.
Abstract Motivation Many Gram-negative bacteria use type VI secretion systems (T6SS) to export effector proteins into adjacent target cells. These secreted effectors (T6SEs) play vital roles in the competitive survival bacterial populations, as well pathogenesis of bacteria. Although various computational analyses have been previously applied identify by certain species, there is no universal method available accurately predict T6SS from growing tide genome sequence data. Results We...
ICOS- and IL-23–mediated costimulation are important for driving in vivo activation of antigen-specific MAIT cells.
The β-barrel assembly machinery (BAM) complex is essential for localization of surface proteins on bacterial cells, but the mechanism by which it functions unclear. We developed a direct stochastic optical reconstruction microscopy (dSTORM) methodology to view BAM in situ. Single-cell analysis showed that discrete membrane precincts housing several complexes are distributed across E. coli surface, with nearest neighbor distance ∼200 nm. auxiliary lipoprotein subunit BamB was crucial this...
Carbapenem-resistant, hypervirulent Klebsiella pneumoniae (CR-hvKP) has recently emerged as a significant threat to public health. In this study, 29 K. isolates were isolated from eight patients admitted the intensive care unit (ICU) of comprehensive teaching hospital located in China March 2017 January 2018. Clinical information was basis for further analyses including antimicrobial susceptibility tests, identification antibiotic resistance and virulence gene determinants, multilocus...
Abstract Even in the setting of optimal resuscitation high-income countries severe sepsis and septic shock have a mortality 20–40%, with antibiotic resistance dramatically increasing this risk. To develop reference dataset enabling identification common bacterial targets for therapeutic intervention, we applied standardized genomic, transcriptomic, proteomic metabolomic technological framework to multiple clinical isolates four sepsis-causing pathogens: Escherichia coli , Klebsiella...