A5041791640- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Electrospun Nanofibers in Biomedical Applications
- Viral Infections and Vectors
- Actinomycetales infections and treatment
- Influenza Virus Research Studies
- Per- and polyfluoroalkyl substances research
- Complex Network Analysis Techniques
- Gastrointestinal motility and disorders
- Plant Pathogenic Bacteria Studies
- Ocular Infections and Treatments
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Clostridium difficile and Clostridium perfringens research
- Phagocytosis and Immune Regulation
- Pluripotent Stem Cells Research
- Microbial metabolism and enzyme function
- Bacterial Identification and Susceptibility Testing
- Escherichia coli research studies
- Gut microbiota and health
- Vagus Nerve Stimulation Research
- Invertebrate Immune Response Mechanisms
- Aquaculture disease management and microbiota
Tiangong University
2025
Xuzhou No.1 People's Hospital
2025
Xuzhou Medical College
2025
The University of Melbourne
2018-2023
Peter Doherty Institute
2018-2023
Zhejiang University
2017-2021
ORCID
2020
University of Hong Kong
2018
Institute of Pharmacology
2017
University of Chinese Academy of Sciences
2011
Abstract Fecal microbiota transplantation (FMT) by manual preparation has been applied to treat diseases for thousands of years. However, this method still endures safety risks and challenges the psychological endurance acceptance doctors, patients donors. Population evidence showed washed with microfiltration based on an automatic purification system followed repeated centrifugation plus suspension three times significantly reduced FMT-related adverse events. This washing makes delivering a...
Mucosal associated invariant T (MAIT) cells are evolutionarily-conserved, innate-like lymphocytes which abundant in human lungs and can contribute to protection against pulmonary bacterial infection. MAIT also activated during viral infections, yet it remains unknown whether play a significant protective or even detrimental role infections vivo. Using murine experimental challenge with two strains of influenza A virus, we show that accumulate early infection, upregulation CD25, CD69 Granzyme...
ICOS- and IL-23–mediated costimulation are important for driving in vivo activation of antigen-specific MAIT cells.
Abstract Mucosal-associated invariant T (MAIT) cells express an TRAV1/TRAJ33 TCR-α chain and are restricted to the MHC-I-like molecule, MR1. Whether MAIT cell development depends on this is unclear. Here we generate Traj33 -deficient mice show that they highly depleted of cells; however, a residual population remains can respond exogenous antigen in vitro or pulmonary Legionella challenge vivo. These include some Trav1 + TCRs with conservative Traj -gene substitutions, others - broad range...
Abstract Mucosal-associated Invariant T (MAIT) cells are recognized for their antibacterial functions. The protective capacity of MAIT has been demonstrated in murine models local infection, including the lungs. Here we show that during systemic infection mice with Francisella tularensis live vaccine strain results evident cell expansion liver, lungs, kidney and spleen peripheral blood. responding manifest a polarised Th1-like MAIT-1 phenotype, transcription factor cytokine profile, confer...
Mucosal-associated invariant T (MAIT) cells detect microbial infection via recognition of riboflavin-based antigens presented by the major histocompatibility complex class I (MHC-I)–related protein 1 (MR1). Most MAIT in human peripheral blood express CD8αα or CD8αβ coreceptors, and binding site for CD8 on MHC-I molecules is relatively conserved MR1. Yet, there no direct evidence interacting with MR1 functional consequences thereof. Similarly, role lymphocyte function remains ill-defined....
Although Tsukamurella infections have been increasingly reported in Europe, Asia, America, and Africa, indicating that diseases caused by this group of bacteria are emerging a global scale, species identification within genus is difficult most clinical microbiology laboratories. Recently, we showed groEL gene sequencing useful for all existing species. Nevertheless, PCR still considered expensive, time-consuming, technically demanding, therefore yet to be incorporated as routine method Using...
Abstract Mucosal‐associated invariant T (MAIT) cells are a major subset of innate‐like mediating protection against bacterial infection through recognition microbial metabolites derived from riboflavin biosynthesis. Mouse MAIT egress the thymus as two main subpopulations with distinct functions, namely, T‐bet‐expressing MAIT1 and RORγt‐expressing MAIT17 cells. Previously, we reported that inducible T‐cell costimulator interleukin (IL)‐23 provide essential signals for optimal MHC‐related...
Abstract Cell death mechanisms in T lymphocytes vary according to their developmental stage, cell subset and activation status. The control of mucosal-associated invariant (MAIT) cells, a specialized population, are largely unknown. Here we report that MAIT cells express key necroptotic machinery; receptor-interacting protein kinase 3 (RIPK3) mixed lineage domain-like (MLKL) protein, abundance. Despite this, discovered the loss RIPK3, but not effector MLKL or apoptotic caspase-8,...
Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong. The strains aerobic, Gram-stain-positive, catalase-positive, non-sporulating non-motile bacilli exhibited unique biochemical profiles distinguishable closely related Tsukamurella species. 16S rRNA gene sequence analysis revealed that shared identical sequences each other, being most to tyrosinosolvens pulmonis, sharing 99.9 % identity. Sequence...
Abstract This unit describes the utility of various mouse models infection and immunization for studying mucosal‐associated invariant T (MAIT) cell immunity: MAIT cells can be isolated from lungs (or other tissues/organs) then identified characterized by flow cytometry using MR1 tetramers in combination with a range antibodies. The response kinetics, cytokine profiles, functional differentiation lung are studied following bacterial pathogen Legionella longbeachae or Salmonella enterica...
Rictor is a key regulatory/structural subunit of the mammalian target rapamycin complex 2 (mTORC2) and required for phosphorylation Akt at serine 473. It plays an important role in cell survival, actin cytoskeleton organization other processes embryogenesis. However, Rictor/mTORC2 embryonic cardiac differentiation has been uncovered. In present study, we examined possible link between expression cardiomyocyte mouse stem (mES) cells. Knockdown by shRNA significantly reduced 473 followed...
Abstract Mucosal associated invariant T (MAIT) cells are evolutionarily-conserved, innate-like lymphocytes which abundant in human lungs and can contribute to protection against pulmonary bacterial infection. MAIT also activated during viral infections, yet it remains unknown whether play a significant protective or even detrimental role infections vivo . Using murine experimental challenge with two strains of influenza A virus, we show that accumulated were early infection, upregulation...
<b>Background:</b> Mucosal associated invariant T (MAIT) cells are evolutionarily-conserved, innate-like lymphocytes which abundant in human lungs and can contribute to protection against pulmonary bacterial infection. However, whilst they also activated during viral infections, it is unknown whether MAIT play a significant protective or even detrimental role infections vivo. <b>Aims objectives:</b> To determine – either influenza A infection <b>Methods:</b> We used major histocompatibility...
为探讨分子伴侣Hfq对溶藻弧菌（Vibrio alginolyticus）毒力的调控作用，本文分析了溶藻弧菌ZJ-T野生株、hfq突变株及回补株各项与毒力相关的生理生化指标。研究结果显示：在半固体和固体LBS平板中，缺失株游动和涌动能力显著降低（P〈0．001）；hfq缺失株生物膜合成速度及合成量降低，但解离速度增加；在限铁环境下，缺失株生长有所减弱；缺失株胞外蛋白酶分泌增强（P〈0．01）；同野生株和突变株相比，缺失株对小鼠成肌细胞系C2C12细胞几乎不致死，且其对石斑鱼的半数致死量提高3个数量级。实验结果表明：Hfq对溶藻弧菌毒力具有重要调控作用，并通过调控其运动、生物膜形成、铁代谢、胞外蛋白酶分泌等生理生化过程从而调控其毒力。本研究可为溶藻弧菌病害暴发的防治提供重要理论依据。
<h3>Background</h3> Mucosal associated invariant T (MAIT) cells are evolutionarily-conserved, innate-like lymphocytes which abundant in human lungs and can contribute to protection against pulmonary bacterial infection. However, whilst they also activated during viral infections, it is unknown whether MAIT play a significant protective or even detrimental role infections<i> vivo</i>. <h3>Aims objectives</h3> To determine – either influenza A infection <i>in <h3>Methods</h3> We used major...
Abstract Mucosal-Associated Invariant T (MAIT) cells have potent antibacterial functions. Their protective capacity, in vivo, has been demonstrated mouse models, particularly of respiratory infections. We now show that during systemic infection mice with Francisella tularensis Live Vaccine Strain (LVS), MAIT cell expansion was evident the liver, lungs, kidney, spleen and blood. manifested a polarised Th1-like (termed “MAIT-1”) phenotype cytokine profile conferred critical role controlling...