A5009919125- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Engineering Education and Curriculum Development
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Eosinophilic Esophagitis
- Inflammatory Bowel Disease
- Immune Response and Inflammation
- Higher Education Learning Practices
- Chemokine receptors and signaling
- Sex and Gender in Healthcare
- Innovative Teaching Methods
- Education and Critical Thinking Development
- Parasites and Host Interactions
- Higher Education and Employability
- Advanced Glycation End Products research
- Single-cell and spatial transcriptomics
- Animal testing and alternatives
- Educational Strategies and Epistemologies
- Retinoids in leukemia and cellular processes
- Evaluation of Teaching Practices
- Escherichia coli research studies
- Cancer Immunotherapy and Biomarkers
- Stress Responses and Cortisol
Peter Doherty Institute
2020-2024
The University of Melbourne
2020-2024
Australian Regenerative Medicine Institute
2016-2023
Monash University
2016-2021
Sheffield Hallam University
2012-2020
Institute of Infection and Immunity
2020
University of Manchester
2014-2019
Discovery Institute
2019
Manchester Academic Health Science Centre
2019
In animal-based biomedical research, both the sex and age of animals studied affect disease phenotypes by modifying their susceptibility, presentation response to treatment. The accurate reporting experimental methods materials, including animals, is essential so that other researchers can build on results such studies. Here we use text mining study 15,311 research papers in which mice were focus study. We find percentage has increased over past two decades: however, only about 50% published...
Abstract Mucosal-associated Invariant T (MAIT) cells are recognized for their antibacterial functions. The protective capacity of MAIT has been demonstrated in murine models local infection, including the lungs. Here we show that during systemic infection mice with Francisella tularensis live vaccine strain results evident cell expansion liver, lungs, kidney and spleen peripheral blood. responding manifest a polarised Th1-like MAIT-1 phenotype, transcription factor cytokine profile, confer...
Programmed cell death 1 (PD1) and cytotoxic T lymphocyte-associated protein 4 (CTLA4) suppress CD4+ activation may promote latent HIV infection. By performing leukapheresis (n = 21) lymph node biopsies 8) in people with on antiretroviral therapy (ART) sorting memory cells into subsets based PD1/CTLA4 expression, we investigate the role of PD1 CTLA persistence. We show that double-positive (PD1+CTLA4+) blood contain more DNA compared double-negative (PD1-CTLA4-) but still have a lower...
The intestine is a unique immune environment that must respond to infectious organisms but remain tolerant commensal microbes and food antigens. However, the molecular mechanisms regulate cell function in unclear. Here we identify POK/ZBTB family transcription factor hypermethylated cancer 1 (HIC1, ZBTB29) as central component of immunity inflammation intestine. HIC1 specifically expressed cells intestinal lamina propria (LP) steady state mice with T-cell-specific deletion have reduced...
CD4+ T helper (Th) cell differentiation is controlled by lineage-specific expression of transcription factors and effector proteins, as well silencing lineage-promiscuous genes. Lysine methyltransferases (KMTs) comprise a major class epigenetic enzymes that are emerging important regulators Th biology. Here, we show the KMT DOT1L regulates function lineage integrity. DOT1L-dependent dimethylation lysine 79 histone H3 (H3K79me2) associated with gene expression. However, DOT1L-deficient cells...
Eosinophils are innate immune cells present in the intestine during steady state conditions. An intestinal eosinophilia is a hallmark of many infections and an accumulation eosinophils also observed inflammatory disorders. Classically function has been associated with tissue destruction, due to release cytotoxic granule contents. However, recent evidence demonstrated that eosinophil plays more diverse role system than previously acknowledged, including shaping adaptive responses providing...
The intestinal immune system must be able to respond a wide variety of infectious organisms while maintaining tolerance non-pathogenic microbes and food antigens. Vitamin A metabolite all-trans-retinoic acid (atRA) has been implicated in the regulation this balance, partially by regulating innate lymphoid cell (ILC) responses intestine. However, molecular mechanisms atRA-dependent immunity homeostasis remain elusive. Here we define role for transcriptional repressor Hypermethylated cancer 1...
Abstract Children in malaria-endemic regions can experience repeated Plasmodium infections over short periods of time. Effects re-infection on multiple co-existing CD4 + T cell subsets remain unresolved. Here, we examine antigen-experienced cells during mice, using scRNA-seq/TCR-seq and spatial transcriptomics. TCR transgenic EM initiate rapid Th1/Tr1 recall responses prior to proliferating, while GC Tfh counterparts are refractory, with CM /Tfh-like exhibiting modest non-proliferative...
Abstract Background Identifying the factors that contribute to chronicity in inflamed colitic tissue is not trivial. However, mouse models of colitis, we can investigate at preclinical timepoints. We sought validate murine Trichuris muris infection as a model for identification promote development chronic colitis. Methods compared changes mice with resolving immune response T. (resistant) vs fail expel worms and develop colitis (susceptible). Findings were then validated healthy controls...
T follicular helper (Tfh) cells are an important component of germinal center (GC)-mediated humoral immunity. Yet, how a chronic type 1 versus protective 2 helminth infection modulates Tfh-GC responses remains poorly understood. Here, we employ the Trichuris muris model and demonstrate that Tfh cell phenotypes GC differentially regulated in acute infection. The latter failed to induce B responses, with expressing Τ-bet interferon-γ. In contrast, interleukin-4-producing dominate acute,...
UK educators often express concerns that students from some cultural backgrounds frequently seem unwilling or are unable to apply critical thinking skills within their academic programmes. This may be due not a lack of ability confidence but rather the way they have been previously taught and assessed. Often, design courses implicitly requires skills, use these in taken into account conceptualization across diverse global group students. paper reports on results study Master's engineering...
ABSTRACT The intestine is a unique immune environment that must respond to infectious organisms but remain tolerant commensal microbes and food antigens. However, the molecular mechanisms regulate cell function in unclear. Here we identify POK/ZBTB family transcription factor Hypermethylated cancer 1 (HIC1, ZBTB29) as central component of immunity inflammation intestine. HIC1 specifically expressed cells intestinal lamina propria (LP) steady state mice with T cell-specific deletion have...
Abstract The interaction between micronutrients and the immune system is emerging as a key control point in cell function homeostasis disease. For example, vitamin A metabolite all-trans retinoic acid (atRA) has established roles migration effector function. Vitamin deficiency results significant reduction intestinal cells, which thought to be associated with lack of homing receptor expression reduced intestine. However, precise molecular pathways controlling remains unclear. Here, we show...
Abstract NOTE: The first page of text has been automatically extracted and included below in lieu an abstract ENGINEERING: THE UK CHALLENGE This paper reviews experiences the development teamwork leadership skills, personal reflection, planning other transferable skills within engineering bachelors degrees over last 15 years, illustrated by examples from one particular University. It provides a picture current state education, reflects upon strengths weaknesses. Comparisons are drawn with...
Abstract NOTE: The first page of text has been automatically extracted and included below in lieu an abstract Business Management the Engineering Curriculum rate change nature employment UK engineering industry commerce greatly increased recent years. large ‘bureaucratic’ organisations public utilities 1970s have replaced with small, dynamic flexible which might only exist for several This clearly requires that we produce graduates who are equipped with, not technical skills, but also skills...
Abstract NOTE: The first page of text has been automatically extracted and included below in lieu an abstract Promoting Learner Autonomy Engineering This paper will report on a current project that is being conducted within one the UK Centres for Excellence Teaching Learning (CETLs): ‘The Centre Autonomy’, at Sheffield Hallam University. outline role CETLs UK, then go to discuss learning, teaching assessment methods used year engineering undergraduate module, order promote learner autonomy...