A5053221368- Enzyme Structure and Function
- Protein Structure and Dynamics
- Nuclear Structure and Function
- Plant-Microbe Interactions and Immunity
- Immune Response and Inflammation
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Biochemical and Molecular Research
- Computational Drug Discovery Methods
- interferon and immune responses
- Legume Nitrogen Fixing Symbiosis
- Bioinformatics and Genomic Networks
- Trace Elements in Health
- Plant Pathogenic Bacteria Studies
- Peptidase Inhibition and Analysis
- Fungal Infections and Studies
- Monoclonal and Polyclonal Antibodies Research
- Transgenic Plants and Applications
- Pneumonia and Respiratory Infections
- Microbial Natural Products and Biosynthesis
- Influenza Virus Research Studies
- Advanced Proteomics Techniques and Applications
- Fungal and yeast genetics research
- Cytokine Signaling Pathways and Interactions
- Glycosylation and Glycoproteins Research
The University of Queensland
2016-2025
Griffith University
2023
Stockholm University
2023
AgriBio
2015-2022
Queens University
2021
Research Institute for Bioscience and Biotechnology
2014-2018
Translational Research Institute
2014
Brisbane School of Theology
2013
Australian e-Health Research Centre
2012
Microsoft (United States)
2011
Plant resistance proteins (R proteins) recognize corresponding pathogen avirulence (Avr) either indirectly through detection of changes in their host protein targets or direct R-Avr interaction. Although indirect recognition imposes selection against Avr effector function, molecules recognized interaction may overcome sequence diversification rather than loss function. Here we show that the flax rust fungus AvrL567 genes, whose products are by L5, L6, and L7 R flax, highly diverse, with 12...
Transition row metal ions are both essential and toxic to microorganisms. Zinc in excess has significant toxicity bacteria, host release of Zn(II) at mucosal surfaces is an important innate defence mechanism. However, the molecular mechanisms by which affords protection have not been defined. We show that Streptococcus pneumoniae extracellular inhibits acquisition Mn(II) competing for binding solute protein PsaA. that, although high-affinity substrate PsaA, can still bind, albeit with a...
NAD depletion as pathogen response One way that plants respond to infection is by sacrificing the infected cells. The nucleotide-binding leucine-rich repeat immune receptors responsible for this hypersensitive carry Toll/interleukin-1 receptor (TIR) domains. In two papers, Horsefield et al. and Wan report these TIR domains cleave metabolic cofactor nicotinamide adenine dinucleotide (NAD + ) part of their cell-death signaling in pathogens. Similar links mammalian TIR-containing proteins...
BIOLOGY IS ENCODED IN MOLECULAR SEQUENCES: deciphering this encoding remains a grand scientific challenge. Functional regions of DNA, RNA, and protein sequences often exhibit characteristic but subtle motifs; thus, computational discovery motifs in is fundamental much-studied problem. However, most current algorithms do not allow for insertions or deletions (indels) within motifs, the few that have other limitations. We present method, GLAM2 (Gapped Local Alignment Motifs), discovering...
Cytoplasmic plant immune receptors recognize specific pathogen effector proteins and initiate effector-triggered immunity. In Arabidopsis, the RPS4 RRS1 are both required to activate defense three different pathogens. We show that physically associate. Crystal structures of N-terminal Toll-interleukin-1 receptor/resistance (TIR) domains RRS1, individually as a heterodimeric complex (respectively at 2.05, 1.75, 2.65 angstrom resolution), reveal conserved TIR/TIR interaction interface. TIR...
Abstract The new European X-ray Free-Electron Laser is the first free-electron laser capable of delivering pulses with a megahertz inter-pulse spacing, more than four orders magnitude higher previously possible. However, to date, it has been unclear whether would indeed be possible measure high-quality diffraction data at pulse repetition rates. Here, we show that structures can obtained using currently available operating conditions XFEL. We present two complete sets, one from well-known...
Cyclic adenosine diphosphate (ADP)–ribose (cADPR) isomers are signaling molecules produced by bacterial and plant Toll/interleukin-1 receptor (TIR) domains via nicotinamide adenine dinucleotide (oxidized form) (NAD + ) hydrolysis. We show that v-cADPR (2′cADPR) v2-cADPR (3′cADPR) cyclized O-glycosidic bond formation between the ribose moieties in ADPR. Structures of 2′cADPR-producing TIR reveal conformational changes lead to an active assembly resembles those Toll-like adaptor domains....
The NADase SARM1 (sterile alpha and TIR motif containing 1) is a key executioner of axon degeneration therapeutic target for several neurodegenerative conditions. We show that potent inhibitor undergoes base exchange with the nicotinamide moiety adenine dinucleotide (NAD
Toll/interleukin-1 receptor (TIR) domain proteins function in cell death and immunity. In plants bacteria, TIR domains are often enzymes that produce isomers of cyclic adenosine 5′-diphosphate–ribose (cADPR) as putative immune signaling molecules. The identity functional conservation cADPR isomer signals is unclear. A previous report found a plant could cross-activate the prokaryotic Thoeris TIR–immune system, suggesting TIR-immune signals. Here, we generate autoactive TIRs test converse...