Richard Bean
A5061405050- Advanced X-ray Imaging Techniques
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Structure and Function
- X-ray Spectroscopy and Fluorescence Analysis
- Particle Accelerators and Free-Electron Lasers
- Photosynthetic Processes and Mechanisms
- Laser-Plasma Interactions and Diagnostics
- Crystallography and Radiation Phenomena
- Photoreceptor and optogenetics research
- X-ray Diffraction in Crystallography
- Nuclear Physics and Applications
- bioluminescence and chemiluminescence research
- Insect Resistance and Genetics
- Electron and X-Ray Spectroscopy Techniques
- Entomopathogenic Microorganisms in Pest Control
- Light effects on plants
- Medical Imaging Techniques and Applications
- Multiferroics and related materials
- Biochemical and Molecular Research
- Protein Structure and Dynamics
- Insect and Pesticide Research
- Microbial Community Ecology and Physiology
- Geophysical and Geoelectrical Methods
- Porphyrin Metabolism and Disorders
- Ion-surface interactions and analysis
European X-Ray Free-Electron Laser
2016-2025
La Trobe University
2022
London Centre for Nanotechnology
2009-2018
University College London
2009-2018
Center for Free-Electron Laser Science
2014-2016
Universität Hamburg
2014-2016
Australian National University
2015
Max Planck Institute for the Structure and Dynamics of Matter
2015
Arizona State University
2015
Deutsches Elektronen-Synchrotron DESY
2013-2015
Accurate knowledge of translation positions is essential in ptychography to achieve a good image quality and the diffraction limited resolution. We propose method retrieve correct position errors during reconstruction iterations. Sub-pixel accuracy after refinement shown be achievable within several tens Simulation experimental results for both optical X-ray wavelengths are given. The improves retrieved object relaxes requirement while acquiring patterns.
Abstract The new European X-ray Free-Electron Laser is the first free-electron laser capable of delivering pulses with a megahertz inter-pulse spacing, more than four orders magnitude higher previously possible. However, to date, it has been unclear whether would indeed be possible measure high-quality diffraction data at pulse repetition rates. Here, we show that structures can obtained using currently available operating conditions XFEL. We present two complete sets, one from well-known...
The European X-ray Free-Electron Laser (FEL) became the first operational high-repetition-rate hard FEL with lasing in May 2017. Biological structure determination has already benefitted from unique properties and capabilities of FELs, predominantly through development application serial crystallography. possibility now performing such experiments at data rates more than an order magnitude greater previous FELs enables not only a higher rate discovery but also new classes previously feasible...
Abstract X-ray free-electron lasers (XFELs) enable novel experiments because of their high peak brilliance and femtosecond pulse duration. However, non-superconducting XFELs offer repetition rates only 10–120 Hz, placing significant demands on beam time sample consumption. We describe serial crystallography performed at the European XFEL, first MHz rate delivering 1.128 trains 10 Hz. Given short spacing between pulses, damage caused by shock waves launched one XFEL probed subsequent pulses...
Abstract The emergence of high repetition-rate X-ray free-electron lasers (XFELs) powered by superconducting accelerator technology enables the measurement significantly more experimental data per day than was previously possible. European XFEL is expected to provide 27,000 pulses second, over two orders magnitude any other XFEL. increased pulse rate a key enabling factor for single-particle diffractive imaging, which relies on averaging weak diffraction signal from single biological...
Here, we illustrate what happens inside the catalytic cleft of an enzyme when substrate or ligand binds on single-millisecond timescales. The initial phase enzymatic cycle is observed with near-atomic resolution using most advanced X-ray source currently available: European XFEL (EuXFEL). high repetition rate EuXFEL combined our mix-and-inject technology enables ceftriaxone binding to
The Sample Environment and Characterization (SEC) group of the European X-ray Free-Electron Laser (EuXFEL) develops sample delivery systems for various scientific instruments, including injection liquid samples that enable serial femtosecond crystallography (SFX) single-particle imaging (SPI) experiments, among others. For rapid prototyping device types materials, sub-micrometre precision 3D printers are used to address specific experimental conditions SFX SPI by providing a large number...
Magnetic domains at the surface of a ferroelectric monodomain BiFeO(3) single crystal have been imaged by hard x-ray magnetic scattering. up to several hundred microns in size observed, corresponding cycloidal modulations magnetization along wave vector k=(δ,δ,0) and symmetry equivalent directions. The rotation direction all domains, determined diffraction circularly polarized light, was found be unique agreement with predictions combined approach based on spin-model complemented...
Abstract Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at Linac Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, aerosolized injected into approximately 0.1 μm diameter focused hard beam CXI LCLS. Diffraction patterns with signal to...
Single particle imaging at x-ray free electron lasers (XFELs) has the potential to determine structure and dynamics of single biomolecules room temperature. Two major hurdles have prevented this from being reached, namely, collection sufficient high-quality diffraction patterns robust computational purification overcome structural heterogeneity. We report breaking both these barriers using gold nanoparticle test samples, recording around 10 million European XFEL structurally orientationally...
Abstract The world’s first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulses. With its rapid rate, XFEL may alleviate some of increasing demand for beamtime, particularly membrane protein serial femtosecond crystallography (SFX), leveraging orders-of-magnitude faster data collection. Here, we report SFX experiment, where determined 2.9 Å-resolution...
The understanding of signal transduction mechanisms in photoreceptor proteins is essential for elucidating how living organisms respond to light as environmental stimuli. In this study, we investigated the ATP binding, photoactivation and process photoactivatable adenylate cyclase from Oscillatoria acuminata (OaPAC) upon blue excitation. Structural models with bound active site native OaPAC at cryogenic well room temperature are presented. found one conformation cryogenic- two conformations...
Dynamics and kinetics in soft matter physics, biology, nanoscience frequently occur on fast (sub)microsecond but not ultrafast timescales which are difficult to probe experimentally. The European X-ray Free-Electron Laser (European XFEL), a megahertz hard source, enables such experiments via taking series of diffraction patterns at repetition rates up 4.5 MHz. Here, we demonstrate photon correlation spectroscopy (XPCS) with submicrosecond time resolution samples the XFEL. We show that XFEL...
The high degree of spatial coherence and extreme pulse energies available at x-ray free electron laser (XFEL) sources naturally support coherent diffractive imaging applications. In order to optimally exploit these unique properties, the optical systems XFELs must be highly transmissive, focus appropriate sizes matched scale samples investigated minimally perturb wavefront XFEL beam. We present design simulated performance two state-of-the-art Kirkpatrik–Baez mirror that form primary foci...
Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into path XFEL, wasting a vast amount due to pulsed nature all current XFEL sources. The European (EuXFEL) delivers (fs) pulses in trains spaced 100 ms apart whereas within are currently separated by 889 ns. Therefore, continuous via fast wastes...
Abstract Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis jegathesan , respectively. The naturally crystallize within host; however, crystals too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. was determined de novo using single-wavelength anomalous dispersion method, which turn...
Pump-probe experiments at X-ray free-electron laser (XFEL) facilities are a powerful tool for studying dynamics ultrafast and longer timescales. Observing the in diverse scientific cases requires optical systems with wide range of wavelength, flexible pulse sequences different durations, especially pump source. Here, pump-probe instrumentation available measurements Single Particles, Clusters, Biomolecules Serial Femtosecond Crystallography (SPB/SFX) instrument European XFEL is reported. The...
X-ray free-electron lasers (XFELs) can probe chemical and biological reactions as they unfold with unprecedented spatial temporal resolution. A principal challenge in this pursuit involves the delivery of samples to interaction point such a way that produces data highest possible quality maximal efficiency. This is hampered by intrinsic constraints posed light source operation within beamline environment. For liquid samples, solution typically some form high-speed jet, capable keeping up...
Free-electron lasers (FEL) are revolutionizing X-ray-based structural biology methods. While protein crystallography is already routinely performed at FELs, Small Angle X-ray Scattering (SAXS) studies of biological macromolecules not as prevalent. SAXS allows the study shape and overall structure proteins nucleic acids in solution, a quasi-native environment. In chemical biophysical parameters that have an influence on dynamics molecules can be varied their effect conformational changes...
The main protease (Mpro) of SARS-CoV-2 is critical for viral function and a key drug target. Mpro only active when reduced; turnover ceases upon oxidation but restored by re-reduction. This suggests the system has evolved to survive periods in an oxidative environment, mechanism this protection not been confirmed. Here, we report crystal structure oxidized showing disulfide bond between site cysteine, C145, distal C117. Previous work proposed provides from irreversible oxidation. forms...
With the use of highly coherent femtosecond X-ray pulses from a free-electron laser, it is possible to record protein nanocrystal diffraction patterns with far more information than present in conventional crystallographic data. It has been suggested that phases may be retrieved such data via iterative algorithms, without priori and restrictions on resolution. Here, we investigate extension this approach nanocrystals edge terminations produce partial unit cells, hence cannot described by...
A major challenge in high-resolution x-ray free-electron laser-based coherent diffractive imaging is the development of aerosol injectors that can efficiently deliver particles to peak intensity focused X-ray beam. Here, we consider use a simple convergent-orifice nozzle for producing tightly beams particles. Through optical show 0.5 μm be full-width at half maximum diameter 4.2 μm, and demonstrate such injecting viruses into micro-focused soft-X-ray FEL
The Lysinibacillus sphaericus proteins Tpp49Aa1 and Cry48Aa1 can together act as a toxin toward the mosquito Culex quinquefasciatus have potential use in biocontrol. Given that with sequence homology to individual activity alone against other insect species, structure of was solved order understand this protein more fully inform design improved biopesticides. is naturally expressed crystalline inclusion within host bacterium, MHz serial femtosecond crystallography using novel nanofocus...
Hydrodynamic cavitation is useful in many processing applications, for example, chemical reactors, water treatment and biochemical engineering. An important type of hydrodynamic that occurs a Venturi tube vortex known to cause luminescence whose intensity closely related the size number events. However, mechanistic origins bubbles constituting remains unclear, although it has been concluded pressure fields generated by collapse strongly depends on bubble geometry. The common view consists...