Andrew V. Martin
A5033549470- Advanced X-ray Imaging Techniques
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Structure and Function
- X-ray Spectroscopy and Fluorescence Analysis
- Crystallography and Radiation Phenomena
- X-ray Diffraction in Crystallography
- Digital Holography and Microscopy
- Laser-Plasma Interactions and Diagnostics
- Photosynthetic Processes and Mechanisms
- Particle Accelerators and Free-Electron Lasers
- nanoparticles nucleation surface interactions
- Spectroscopy and Quantum Chemical Studies
- Archaeology and Natural History
- Electron and X-Ray Spectroscopy Techniques
- Archaeology and ancient environmental studies
- Quantum Information and Cryptography
- Material Dynamics and Properties
- Protein Structure and Dynamics
- Astrophysical Phenomena and Observations
- Lipid Membrane Structure and Behavior
- Ionic liquids properties and applications
- Historical and Cultural Archaeology Studies
- Liquid Crystal Research Advancements
- Nuclear Physics and Applications
- Cold Atom Physics and Bose-Einstein Condensates
RMIT University
2018-2025
MIT University
2021-2023
Lawrence Berkeley National Laboratory
2022
ARC Centre of Excellence in Advanced Molecular Imaging
2015-2020
The University of Melbourne
2009-2020
Uppsala University
2019
Universität Hamburg
2010-2016
Center for Free-Electron Laser Science
2010-2016
ARC Centre of Excellence for Coherent X-ray Science
2012-2015
Deutsches Elektronen-Synchrotron DESY
2011-2014
Size Matters Less X-ray crystallography is a central research tool for uncovering the structures of proteins and other macromolecules. However, its applicability typically requires growth large crystals, in part because sufficient number molecules must be present lattice sample to withstand x-ray—induced damage. Boutet et al. (p. 362 , published online 31 May) now demonstrate that intense x-ray pulses emitted by free-electron laser source can yield data few enough exposures uncover...
In order to address the specific needs of emerging technique `serial femtosecond crystallography', in which structural information is obtained from small crystals illuminated by an X-ray free-electron laser, a new software suite has been created. The constituent programs deal with viewing, indexing, integrating, merging and evaluating quality data, also simulating patterns. challenges addressed chiefly concern indexing integration large numbers diffraction patterns automated manner, so...
The Trypanosoma brucei cysteine protease cathepsin B (TbCatB), which is involved in host protein degradation, a promising target to develop new treatments against sleeping sickness, fatal disease caused by this protozoan parasite. structure of the mature, active form TbCatB has so far not provided sufficient information for design safe and specific drug T. brucei. By combining two recent innovations, vivo crystallization serial femtosecond crystallography, we obtained room-temperature 2.1...
We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns an x-ray free-electron laser. Three-dimensional imaging requires assembly many two-dimensional into internally consistent Fourier volume. Since each is randomly oriented when exposed to pulse, relative orientations have be retrieved data alone. achieve this with modified version expand, maximize and compress algorithm validate our result using new...
We demonstrate the use of an X-ray free electron laser synchronized with optical pump to obtain diffraction snapshots from photoactivated states large membrane protein complexes in form nanocrystals flowing a liquid jet.Light-induced changes Photosystem I-Ferredoxin co-crystals were observed at time delays 5 10 µs after excitation.The result correlates microsecond kinetics transfer I ferredoxin.The undocking process that follows leads rearrangements crystals will terminally lead...
We report experimental results on x-ray diffraction of quantum-state-selected and strongly aligned ensembles the prototypical asymmetric rotor molecule 2,5-diiodobenzonitrile using Linac Coherent Light Source. The experiments demonstrate first steps toward a new approach to diffractive imaging distinct structures individual, isolated gas-phase molecules. confirm several key ingredients single experiments: abilities detect count individual scattered photons in shot data, deliver...
There exists a conspicuous gap of knowledge about the organization life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that introduce beam aerosolised cyanobacteria into focus Linac Coherent Light Source record diffraction patterns from individual cells very low noise levels high hit ratios. We obtain two-dimensional...
X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction sample by radiation damage. Diffraction intensities lysozyme collected at Linac Coherent Light Source using 2 keV photons were used for structure determination molecular replacement and analyzed damage as a function pulse length fluence. Signatures are observed short 70 fs. Parametric scaling in conventional crystallography does not...
A complete set of structure factors has been extracted from hundreds thousands femtosecond single-shot X-ray microdiffraction patterns taken randomly oriented nanocrystals. The method Monte Carlo integration over crystallite size and orientation was applied to experimental data Photosystem I This arrives at many partial reflections without prior knowledge the particle-size distribution. were collected Linac Coherent Light Source (the first hard-X-ray laser user facility), which fitted a...
We propose a many-body quantum-mechanical model for multiple thermal scattering of fast electrons due to phonon excitation in condensed matter. The key approximation upon which our approach is based the spirit Born-Oppenheimer approximation. compare conceptual underpinnings this well-known frozen scattering. Using it possible calculate probability distribution both elastically and inelastically scattered electrons. also extended encompass core-shell ionization.
We present an analysis of ice nucleation kinetics from near-ambient pressure water as temperature decreases below the homogeneous limit TH by cooling micrometer-sized droplets (microdroplets) evaporatively at 103-104 K/s and probing structure ultrafast using femtosecond pulses Linac Coherent Light Source (LCLS) free-electron X-ray laser. Below 232 K, we observed a slower rate increase with decreasing than anticipated previous measurements, which suggest is due to rapid decrease in water's...
X-ray free-electron lasers (XFELs) enable crystallographic structure determination beyond the limitations imposed upon synchrotron measurements by radiation damage. The need for very short XFEL pulses is relieved through gating of Bragg diffraction loss crystalline order as damage progresses, but not if ionization events are spatially non-uniform due to underlying elemental distributions, in biological samples. Indeed, correlated movements iron and sulfur ions were observed XFEL-irradiated...
Single-particle experiments using X-ray Free Electron Lasers produce more than 10(5) snapshots per hour, consisting of an admixture blank shots (no particle intercepted), and exposures one or particles. Experimental data sets also often contain unintentional contamination with different species. We present unsupervised method able to sort experimental without recourse templates, specific noise models, user-directed learning. The results show 90% agreement manual classification.
The resolution of single-shot coherent diffractive imaging at X-ray free-electron laser facilities is limited by the low signal-to-noise level diffraction data high scattering angles.The iterative reconstruction methods, which phase a continuous pattern to produce an image, must be able extract information from these weak signals obtain best quality images.Here we show how modify methods improve tolerance noise.The method demonstrated with hybrid input-output on both simulated and patterns...