A5101494330- Advanced X-ray Imaging Techniques
- Enzyme Structure and Function
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Electron Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Plasmonic and Surface Plasmon Research
- Advanced Glycation End Products research
- Copper-based nanomaterials and applications
- Transition Metal Oxide Nanomaterials
- Advanced biosensing and bioanalysis techniques
- X-ray Spectroscopy and Fluorescence Analysis
- Alzheimer's disease research and treatments
- Gene expression and cancer classification
- Galectins and Cancer Biology
- Genomic variations and chromosomal abnormalities
- Consumer Perception and Purchasing Behavior
- Fungal and yeast genetics research
- Nanocluster Synthesis and Applications
- Magnetic Properties and Synthesis of Ferrites
- Advanced Optical Imaging Technologies
- Biochemical and Molecular Research
- scientometrics and bibliometrics research
- Microfluidic and Bio-sensing Technologies
- Advanced Neuroimaging Techniques and Applications
- Biochemical effects in animals
Seoul National University
2006-2024
European X-Ray Free-Electron Laser
2018-2023
University of Seoul
2021
Daegu-Gyeongbuk Medical Innovation Foundation
2021
National Institutes of Health
2007
National Human Genome Research Institute
2007
Objective Cerebral amyloidosis and severe tauopathy in the brain are key pathological features of Alzheimer’s disease (AD). Despite a strong influence intestinal microbiota on AD, causal relationship between gut AD pathophysiology is still elusive. Design Using recently developed AD-like pathology with amyloid neurofibrillary tangles (ADLP APT ) transgenic mouse model which shows plaques, reactive gliosis their brains along memory deficits, we examined impact pathogenesis. Results...
Abstract X-ray free-electron lasers (XFELs) enable novel experiments because of their high peak brilliance and femtosecond pulse duration. However, non-superconducting XFELs offer repetition rates only 10–120 Hz, placing significant demands on beam time sample consumption. We describe serial crystallography performed at the European XFEL, first MHz rate delivering 1.128 trains 10 Hz. Given short spacing between pulses, damage caused by shock waves launched one XFEL probed subsequent pulses...
Abstract The world’s first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulses. With its rapid rate, XFEL may alleviate some of increasing demand for beamtime, particularly membrane protein serial femtosecond crystallography (SFX), leveraging orders-of-magnitude faster data collection. Here, we report SFX experiment, where determined 2.9 Å-resolution...
Ag nanoparticles have garnered significant attention for their excellent plasmonic properties and potential use as cavities, primarily because of intrinsically low ohmic losses optical in the visible range. These are particularly crucial systems involving quantum dots that absorb light at wavelengths, where need a high threshold energy interband transitions necessitates incorporation nanostructures. However, synthesis still encounters challenges achieving structural uniformity...
ConspectusPlasmonic metal nanostructures have been extensively developed over the past few decades because of their ability to confine light within surfaces and manipulate strong light-matter interactions. The energy stored by plasmonic nanomaterials in form surface plasmons can be utilized initiate chemical reactions, so-called plasmon-induced catalysis, which stresses importance understanding chemistry materials. Nevertheless, only physical interpretation behaviors has a dominant theme,...
Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into path XFEL, wasting a vast amount due to pulsed nature all current XFEL sources. The European (EuXFEL) delivers (fs) pulses in trains spaced 100 ms apart whereas within are currently separated by 889 ns. Therefore, continuous via fast wastes...
Free-electron lasers (FEL) are revolutionizing X-ray-based structural biology methods. While protein crystallography is already routinely performed at FELs, Small Angle X-ray Scattering (SAXS) studies of biological macromolecules not as prevalent. SAXS allows the study shape and overall structure proteins nucleic acids in solution, a quasi-native environment. In chemical biophysical parameters that have an influence on dynamics molecules can be varied their effect conformational changes...
The receptor for advanced glycation end products (RAGE) has been found to interact with amyloid β (Aβ). Although RAGE does not have any kinase motifs in its cytosolic domain, the interaction between and Aβ triggers multiple cellular signaling involved Alzheimer’s disease (AD). However, mechanism of signal transduction by remains still unknown. Therefore, identifying binding proteins may provide novel therapeutic targets AD. In this study, we identified p38-regulated/activated protein (PRAK)...
Abstract The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive pulses collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure XFEL obtain two complete datasets same lysozyme crystal, first hit second hit, before exits...
Here, we reviewed the electrochromic behaviour and underlying mechanisms of plasmonic metal nanoparticles in visible spectral range, discussed recent key progresses understanding, controlling utilizing electrochromism.
Plasmonic electrochromism, a change in the localized surface plasmon resonance (LSPR) with an applied electric potential, has been attracting increasing attention for development of spectroscopic tools or optoelectronic systems. There is consensus on mechanism plasmonic electrochromism based classical capacitor and Drude model. However, electrochromic behaviors metallic nanoparticles narrow optical windows have demonstrated only small monotonic LSPR shifts, which limits use electrochromism....
Abstract "Genetical genomics", the study of natural genetic variation combining data from marker-based studies with gene expression analyses, has exploded recent development advanced microarray technologies. To account for systematic known to exist in data, it is critical properly normalize traits before performing linkage analyses. However, imposing equal means and variances across pedigrees can over-correct true biological by ignoring familial correlations values. We applied robust...
Copy number variation (CNV) is a primary source of structural in the human genome, leading to several disorders. Therefore, analyzing neonatal CNVs crucial for managing CNV-related chromosomal disabilities. However, genomic waves can hinder accurate CNV analysis. To mitigate influences waves, we adopted machine learning approach and developed new method that uses modified log R ratio instead commonly used ratio. Validation results using samples with known demonstrated superior performance...
We provide a detailed description of serial femtosecond crystallography (SFX) dataset collected at the European X-ray free-electron laser facility (EuXFEL). The EuXFEL is first high repetition rate XFEL delivering MHz pulse trains 10 Hz. short spacing (<1 µs) between pulses requires fast flowing microjets for sample injection and frame detectors. A data set was recorded microcrystalline mixture least three different jack bean proteins (urease, concanavalin A, B). one megapixel Adaptive Gain...
Nanoparticles, exhibiting functionally relevant structural heterogeneity, are at the forefront of cutting-edge research. Now, high-throughput single-particle imaging (SPI) with x-ray free-electron lasers (XFELs) creates unprecedented opportunities for recovering shape distributions millions particles that exhibit heterogeneity. To realize this potential, three challenges have to be overcome: (1) simultaneous parametrization variability in real and reciprocal spaces; (2) efficiently inferring...
Blood cell counting typically requires complex machinery. Flow cytometers used for this purpose involve precise optical alignment, costly detectors, and pretreatment with fluorescent labels. Coulter countertype devices, which monitor ion current, are simpler. However, conventional counters provide only information about size, making it impossible to distinguish similarly sized lymphocytes from red blood cells (RBCs). Inspired by the fact that RBCs have an exceptionally high propensity absorb...
One of the outstanding analytical problems in X-ray single particle imaging (SPI) is classification structural heterogeneity, which especially difficult given low signal-to-noise ratios individual patterns and that even identical objects can yield vary greatly when orientation taken into consideration. We propose two methods explicitly account for this orientation-induced variation robustly determine landscape a sample ensemble. The first, termed common-line principal component analysis...