A5058794433- Photosynthetic Processes and Mechanisms
- Metalloenzymes and iron-sulfur proteins
- Trace Elements in Health
- Microbial Fuel Cells and Bioremediation
- Bacterial Infections and Vaccines
- Metal-Catalyzed Oxygenation Mechanisms
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Pneumonia and Respiratory Infections
- Redox biology and oxidative stress
- Electrocatalysts for Energy Conversion
- Streptococcal Infections and Treatments
- Photoreceptor and optogenetics research
- Wastewater Treatment and Nitrogen Removal
- Radioactive element chemistry and processing
- Algal biology and biofuel production
- Antimicrobial Resistance in Staphylococcus
- Bacterial Genetics and Biotechnology
- Porphyrin Metabolism and Disorders
- Genomics and Phylogenetic Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial biofilms and quorum sensing
- Marine and coastal ecosystems
- Reproductive tract infections research
- Heme Oxygenase-1 and Carbon Monoxide
The University of Queensland
2014-2024
AgriBio
2024
Griffith University
2015
Princess Alexandra Hospital
2006
University of East Anglia
1991-2002
Stony Brook University
2000
Daresbury Laboratory
1999
University of Sussex
1999
University of Oxford
1990-1993
John Innes Centre
1993
Transition row metal ions are both essential and toxic to microorganisms. Zinc in excess has significant toxicity bacteria, host release of Zn(II) at mucosal surfaces is an important innate defence mechanism. However, the molecular mechanisms by which affords protection have not been defined. We show that Streptococcus pneumoniae extracellular inhibits acquisition Mn(II) competing for binding solute protein PsaA. that, although high-affinity substrate PsaA, can still bind, albeit with a...
Short-chain fatty acids are known modulators of host-microbe interactions and can affect human health, inflammation, outcomes microbial infections. Acetate is the most abundant but least well-studied these modulators, with studies focusing on propionate butyrate, which considered to be more potent. In this mini-review, we summarize current knowledge acetate as an important anti-inflammatory modulator between hosts microorganisms. This includes a summary pathways by metabolized bacteria...
Recent discoveries relating to pathways of anaerobic electron transport in the Rhodospirillaceae are reviewed. The main emphasis is on organism Rhodobacter capsulatus** but comparisons made with sphaeroides** f. sp. denitrificans and Rhodopseudomonas palustris. known acceptors for respiration capsulatus trimethylamine-N-oxide (TMAO), dimethyl sulphoxide (DMSO), nitrate nitrous oxide. In each case generates a proton electrochemical gradient some cases can support growth non-fermentable carbon...
The movement of key transition metal ions is recognized to be critical importance in the interaction between macrophages and intracellular pathogens. present study investigated role copper mouse macrophage responses Salmonella enterica sv. Typhimurium. chelator BCS (bathocuproinedisulfonic acid, disodium salt) increased survival S. Typhimurium within primary BMM (bone-marrow-derived macrophages) at 24 h post-infection, implying that contributed effective host defence against this pathogen....
Zinc plays an important role in human immunity, and it is known that zinc deficiency the host linked to increased susceptibility bacterial infection. In this study, we investigate of efflux pathogenesis Streptococcus pyogenes (group A [GAS]), a pathogen responsible for superficial infections, such as pharyngitis impetigo, severe invasive infections.The clinically M1T1 wild-type strain was used isogenic mutants were constructed with deletions czcD gene (Spy0653; which encodes putative pump)...
Streptococcus pneumoniae requires manganese for colonization of the human host, but underlying molecular basis this requirement has not been elucidated. Recently, it was shown that zinc could compromise uptake and levels increased during infection by S. in all niches colonized. Here we show, quantitative means, extracellular acts a dose dependent manner to competitively inhibit pneumoniae, with an EC50 30.2 µM cation-defined media. By exploiting ability directly manipulate accumulation...
Abstract Cadmium is a transition metal ion that highly toxic in biological systems. Although relatively rare the Earth’s crust, anthropogenic release of cadmium since industrialization has increased biogeochemical cycling and abundance biosphere. Despite this, molecular basis its toxicity remains unclear. Here we combine metal-accumulation assays, high-resolution structural data biochemical analyses to show toxicity, Streptococcus pneumoniae , occurs via perturbation first row homeostasis....
Abstract Non-typeable Haemophilus influenzae contains an N 6 -adenine DNA-methyltransferase (ModA) that is subject to phase-variable expression (random ON/OFF switching). Five modA alleles, modA2 , modA4, modA5 modA9 and modA10 account for over two-thirds of clinical otitis media isolates surveyed. Here, we use single molecule, real-time (SMRT) methylome analysis identify the DNA-recognition motifs all five these alleles. Phase variation alleles regulates multiple proteins including vaccine...
Abstract Neutrophils release free zinc to eliminate the phagocytosed bacterial pathogen Streptococcus pyogenes (Group A ; GAS). In this study, we investigated mechanisms underpinning toxicity towards human pathogen, responsible for diseases ranging from pharyngitis and impetigo, severe invasive infections. Using globally-disseminated M1T1 GAS strain, demonstrate that stress impairs glucose metabolism through inhibition of glycolytic enzymes phosphofructokinase glyceraldehyde-3-phosphate...
ABSTRACT Streptococcus pyogenes (group A [GAS]) is an obligate human pathogen responsible for a spectrum of disease states. Metallobiology pathogens revealing the fundamental role metals in both nutritional immunity leading to starvation and metal poisoning by innate immune cells. Spy0980 (MntE) paralog GAS zinc efflux pump CzcD. Through use isogenic mntE deletion mutant serotype M1T1 strain 5448, we have elucidated that MntE manganese-specific required virulence. The 5448Δ had significantly...
Direct oxidation of sulfite to sulfate occurs in various photo- and chemotrophic sulfur oxidizing microorganisms as the final step reduced compounds is catalyzed by sulfite:cytochrome c oxidoreductase (EC ). Here we show that enzyme from Thiobacillus novellus a periplasmically located alphabeta heterodimer, consisting 40.6-kDa subunit containing molybdenum cofactor an 8.8-kDa mono-heme cytochrome c(552) (midpoint redox potential, E(m8.0) = +280 mV). The organic component was identified...
As a facultative aerobe with high iron requirement and highly active aerobic respiratory chain, Neisseria gonorrhoeae requires defence systems to respond toxic oxygen species such as superoxide. It has been shown that supplementation of media 100 µM Mn(II) considerably enhanced the resistance this bacterium oxidative killing by This protection was not associated superoxide dismutase enzymes N. . In contrast previous studies, which suggested some strains might contain dismutase, we identified...
ABSTRACT psaA encodes a 37-kDa pneumococcal lipoprotein which is part of an ABC Mn(II) transport complex. Streptococcus pneumoniae D39 mutants have previously been shown to be significantly less virulent than wild-type D39, but the mechanism underlying attenuation has not resolved. In this study, we that and psaD are highly sensitive oxidative stress, i.e., superoxide hydrogen peroxide, might explain why they strain. Our investigations revealed altered expression key oxidative-stress...
The Rhodobacter sphaeroides cytochrome c2 functions as a mobile electron carrier in both aerobic and photosynthetic transport chains. Synthetic deoxyoligonucleotide probes, based on the known amino acid sequence of this protein (Mr 14,000), were used to identify clone structural gene (cycA). DNA analysis cycA indicated presence typical procaryotic 21-residue signal sequence, suggesting that periplasmic is synthesized vivo precursor. Synthesis an immunoreactive precursor 15,500) was observed...
Summary The psaBCA locus of Streptococcus pneumoniae encodes a putative ABC Mn 2+ ‐permease complex. Downstream the operon is psaD , which may be co‐transcribed and thiol peroxidase. Previously, there has been discordance concerning phenotypic impact mutations in psa locus, resolution complicated by differences mutant construction possibility polar effects. Here, we constructed unmarked, frame deletion mutants Δ psaB psaC psaA psaBC psaBCAD S. D39 to examine role each gene within uptake,...
Pedomicrobium sp. ACM 3067 is a budding-hyphal bacterium belonging to the alpha-Proteobacteria which able oxidize soluble Mn2+ insoluble manganese oxide. A cosmid, from whole-genome library, containing putative genes responsible for oxidation was identified and primer-walking approach yielded 4350 bp of novel sequence. Analysis this sequence showed presence predicted three-gene operon, moxCBA. The moxA gene product homology multicopper oxidases (MCOs) contained characteristic four...
Catheter-associated urinary tract infection (CAUTI) is the most common nosocomial in United States. Uropathogenic Escherichia coli (UPEC), cause of CAUTI, can form biofilms on indwelling catheters. Here, we identify and characterize novel factors that affect biofilm formation by UPEC strains CAUTI. Sixty-five CAUTI isolates were characterized for phenotypic markers urovirulence, including agglutination formation. One isolate, E. MS2027, was uniquely proficient at growth despite absence...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhenotypic and genetic characterization of cytochrome c2-deficient mutants Rhodobacter sphaeroidesTimothy J. Donohue, Alastair G. McEwan, Steven Van Doren, Antony R. Crofts, Samuel KaplanCite this: Biochemistry 1988, 27, 6, 1918–1925Publication Date (Print):March 22, 1988Publication History Published online1 May 2002Published inissue 22 March 1988https://pubs.acs.org/doi/10.1021/bi00406a018https://doi.org/10.1021/bi00406a018research-articleACS...