A5070226793- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Enzyme Structure and Function
- RNA modifications and cancer
- Redox biology and oxidative stress
- CRISPR and Genetic Engineering
- Streptococcal Infections and Treatments
- Sulfur Compounds in Biology
The University of Queensland
2020-2023
The autotransporter protein secretion system has been used previously to target the of heterologous proteins bacterial cell surface and extracellular milieu at laboratory scale. platform is particular interest for production "difficult" recombinant that might cause toxic effects when produced intracellularly. One such IrmA. IrmA a vaccine candidate in inclusion bodies requiring refolding. Here, we describe use scale-up an industrially relevant (IrmA). A plasmid expressing was constructed...
Protein acylation is critical for many cellular functions across all domains of life. In bacteria, lipoproteins have important roles in virulence and are targets the development antimicrobials vaccines. Bacterial secreted from cytosol via Sec pathway acylated on an N-terminal cysteine residue through action three enzymes. Gram-negative Lol transports to outer membrane. Here, we demonstrate that Aat secretion system a composite sharing similarity with elements type I systems pathway. During...
Abstract Small or overlapping genes are prevalent across all domains of life but often overlooked for annotation and function because challenges in their detection. The advent high-density mutagenesis data-mining studies suggest the existence further coding potential within bacterial genomes. To overcome limitations existing protein detection methods, we applied a genetics-based approach. We combined transposon insertion sequencing with translation reporter to identify translated open...
Abstract Protein acylation is critical for many cellular functions including signal transduction, cell division and development. In bacteria, such lipoproteins have important roles in virulence are therefore potential targets the development of novel antimicrobials vaccines. To date, all known bacterial secreted from cytosol via Sec pathway, acylated on an N-terminal cysteine residue through action Lgt, Lsp Lnt, then targeted to appropriate location. case Gram-negative lipoprotein...