A5069398982- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Genomics and Chromatin Dynamics
- Plant Pathogens and Resistance
- Mycorrhizal Fungi and Plant Interactions
- DNA Repair Mechanisms
- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Genomics and Phylogenetic Studies
- Genome Rearrangement Algorithms
- Plant and fungal interactions
- Nail Diseases and Treatments
- DNA and Nucleic Acid Chemistry
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
Université de Montréal
2020-2025
Jawaharlal Nehru Centre for Advanced Scientific Research
2018-2021
Institute of Bioinformatics and Applied Biotechnology
2018
Biotech Park
2018
Significance The “centromere paradox” refers to rapidly evolving and highly diverse centromere DNA sequences even in closely related eukaryotes. However, factors contributing this rapid divergence are largely unknown. Here, we identified large regional, LTR retrotransposon-rich centromeres a group of human fungal pathogens belonging the Cryptococcus species complex. We provide evidence that loss-of-functional RNAi machinery possibly cytosine methylation trigger instability genome by...
Genomic rearrangements associated with speciation often result in variation chromosome number among closely related species. Malassezia species show variable karyotypes ranging between six and nine chromosomes. Here, we experimentally identified all eight centromeres M. sympodialis as 3–5-kb long kinetochore-bound regions that span an AT-rich core are depleted of the canonical histone H3. Centromeres similar sequence features were CENP-A-rich furfur, which has seven chromosomes, H3 slooffiae...
ABSTRACT We report the assembly and annotation of nuclear genome Diaporthe vaccinii , a pathogenic fungus isolated from diseased cranberries in Quebec, Canada. The was sequenced with Illumina paired-end sequencing technology, assembled into 67 Mbp across 588 contigs, an N50 386 Kbp 97.5% BUSCO completeness.
Centromeres are chromosomal regions that serve as platforms for kinetochore assembly and spindle attachments, ensuring accurate chromosome segregation during cell division. Despite functional conservation, centromere DNA sequences diverse often repetitive, making them challenging to assemble identify. Here, we describe centromeres in an oomycete Phytophthora sojae by combining long-read sequencing-based genome chromatin immunoprecipitation the centromeric histone CENP-A followed...
Centromeres of Candida albicans form on unique and different DNA sequences but a closely related species, tropicalis, possesses homogenized inverted repeat (HIR)-associated centromeres. To investigate the mechanism centromere type transition, we improved fragmented genome assembly constructed chromosome-level C. tropicalis by employing PacBio sequencing, chromosome conformation capture sequencing (3C-seq), chromoblot, genetic analysis engineered aneuploid strains. Further, analyzed 3D...
Abstract The molecular cues for CENPA positioning in epigenetically regulated centromeres is elusive. Candida albicans has small regional non-repetitive that do not harbor conventional heterochromatin. Deletion of native centromere leads to activation a neocentromere... diploid budding yeast harbors unique CENPA-rich 3- 5-kb regions form the (CEN) core on each its eight chromosomes. epigenetic nature these CENs does permit stabilization functional kinetochore an exogenously introduced CEN...
The establishment of centromeric chromatin and its propagation by the centromere-specific histone CENPA is mediated epigenetic mechanisms in most eukaryotes. DNA replication origins, origin binding proteins, timing centromere are important determinants function. epigenetically regulated regional centromeres budding yeast Candida albicans have unique sequences that replicate earliest every chromosome clustered throughout cell cycle. In this study, genome-wide occupancy initiation protein Orc4...
Abstract Spatiotemporal regulation in DNA replication maintains kinetochore stability. The epigenetically regulated centromeres (CENs) the budding yeast Candida albicans have unique sequences, replicate early and are clustered throughout cell cycle. In this study, genome-wide occupancy of initiation protein Orc4 reveals its abundance at all CENs C. . associates with four different motifs, one which coincides tRNA genes. Hi-C combined timing analyses identify enriched interactions among or...
Ericaceae thrive in poor soil, which we postulate is facilitated by microbes living inside those plants. Here, investigate the growth stimulation of American cranberry (Vaccinium macrocarpon) one its fungal endosymbionts, EC4. We show that symbiont resides epidermal root cells host but extends into rhizosphere via hyphae. Morphological classification this fungus ambiguous, phylogenetic inference based on 28S rRNA identifies EC4 as a Codinaeella species (Chaetosphaeriaceae, Sordariomycetes,...
ESBL-producing isolates of the Enterobacteriaceae occur throughout world. The objectives this study were to characterize uropathogenic Escherichia coli isolated at a tertiary care hospital in southern India, and shed light on blaCTX-M sequences Indian origin. A cohort 13 urinary E. (obtained from patients Sri Sathya Sai Institute Higher Medical Sciences, Prasanthigram, Andhra Pradesh, India) characterized found be resistant multiple antibiotics, including extended-spectrum cephalosporins....
Fungi colonizing plants are gaining attention because of their ability to promote plant growth and suppress pathogens. While most studies focus on endosymbionts from grasses legumes, the large diverse group ericaceous has been much neglected. We recently described one very few fungal endophytes promoting Ericaceae Vaccinium macrocarpon (American cranberry), notably Codinaeella isolate EC4. Here, we show that EC4 also suppresses pathogens, which makes it a promising endophyte for sustainable...
Abstract Centromeres are chromosomal regions that serve as platforms for kinetochore assembly and spindle attachments, ensuring accurate chromosome segregation during cell division. Despite functional conservation, centromere DNA sequences diverse often repetitive, making them challenging to assemble identify. Here, we describe centromeres in an oomycete Phytophthora sojae by combining long-read sequencing-based genome chromatin immunoprecipitation the centromeric histone CENP-A followed...
Abstract Centromeres of Candida albicans form on unique and different DNA sequences but a closely related species, tropicalis , possesses homogenized inverted repeat (HIR)-associated centromeres. To investigate the mechanism centromere-type transition, we improved fragmented genome assembly constructed chromosome-level C. by employing PacBio sequencing, chromosome conformation capture sequencing (3C-seq), chromoblot, genetic analysis engineered aneuploid strains. Further, analyzed 3D...
Abstract Intra-chromosomal or inter-chromosomal genomic rearrangements often lead to speciation (1). Loss gain of a centromere leads alterations in chromosome number closely related species. Thus, centromeres can enable tracing the path evolution from ancestral derived state (2). The Malassezia species complex phylum Basiodiomycota shows remarkable diversity ranging between six and nine chromosomes (3–5). To understand these transitions, we experimentally identified all eight as binding...