A5047682706- Plant Pathogens and Resistance
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Fungal Diseases
- Genetic Mapping and Diversity in Plants and Animals
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Fungal Infections and Studies
- Autophagy in Disease and Therapy
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Plant Reproductive Biology
- Epigenetics and DNA Methylation
- Insect-Plant Interactions and Control
- Smoking Behavior and Cessation
- Plant Pathogenic Bacteria Studies
- COVID-19 epidemiological studies
- Genomics and Chromatin Dynamics
- Reproductive biology and impacts on aquatic species
- Genomics, phytochemicals, and oxidative stress
- Energy Load and Power Forecasting
- Forecasting Techniques and Applications
- School Health and Nursing Education
GreenLight Biosciences (United States)
2022-2025
Research Triangle Park Foundation
2024-2025
Université Bourgogne Franche-Comté
2025
Duke Medical Center
2017-2024
Duke University Hospital
2017-2024
Australian National University
2021
Duke University
2019
Oregon State University
2015-2017
Virginia Tech
2015-2017
Computational Physics (United States)
2015
Phytophthora sojae is an oomycete pathogen of soybean. As a result its economic importance, P. has become model for the study genetics, physiology and pathology. The lack efficient techniques targeted mutagenesis gene replacement have long hampered genetic studies pathogenicity in species. Here, we describe CRISPR/Cas9 system enabling rapid genome editing sojae. Using RXLR effector Avr4/6 as target, observed that, absence homologous template, repair Cas9-induced DNA double-strand breaks...
The extracellular space (apoplast) of plant tissue represents a critical battleground between plants and attacking microbes. Here we show that pathogen-secreted apoplastic xyloglucan-specific endoglucanase, PsXEG1, is focus this struggle in the Phytophthora sojae-soybean interaction. We soybean produces an glucanase inhibitor protein, GmGIP1, binds to PsXEG1 block its contribution virulence. P. sojae, however, secretes paralogous PsXEG1-like PsXLP1, has lost enzyme activity but GmGIP1 more...
Abstract Phytophthora is a filamentous fungus‐like microorganism, but belongs to the oomycetes, in kingdom Stramenopila. species are notorious as plant destroyers, causing multibillion‐dollar damage agriculture and natural ecosystems worldwide annually. For long time, genome editing has been unattainable because of their extremely low rate homologous recombination. The recent implementation CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR‐associated) system...
Oomycete protists share phenotypic similarities with fungi, including the ability to cause plant diseases, but branch in a distant region of tree life. It has been suggested that multiple horizontal gene transfers (HGTs) from fungi-to-oomycetes contributed evolution plant-pathogenic traits. These HGTs are predicted include secreted proteins degrade cell walls, barrier pathogen invasion and rich source carbohydrates. Using combination phylogenomics functional assays, we investigate...
Smoking prevalence is high in China and even higher among rural residents. The aims of this study were: 1) to gain insights into the motivations tobacco use barriers smoking cessation village residents; 2) understand current control measures villages encountered or perceived for implementation. Qualitative semi-structured face-to-face interviews focus group discussions were conducted 59 villagers including 37 residents, 10 leaders 12 doctors three counties Shandong Province, China....
Centromeres are chromosomal regions that serve as platforms for kinetochore assembly and spindle attachments, ensuring accurate chromosome segregation during cell division. Despite functional conservation, centromere DNA sequences diverse often repetitive, making them challenging to assemble identify. Here, we describe centromeres in an oomycete Phytophthora sojae by combining long-read sequencing-based genome chromatin immunoprecipitation the centromeric histone CENP-A followed...
Phytophthora is a long-established, well known and globally important genus of plant pathogens. Phylogenetic evidence has shown that the biologically distinct, obligate biotrophic downy mildews evolved from at least twice. Since, cladistically, this renders ‘paraphyletic’, it been proposed evolutionary clades be split into multiple genera (Runge et al. 2011; Crous 2021; Thines 2023; 2024). In letter, we review arguments for retention generic name with broad circumscription made by Brasier...
To date, nuclear localization signals (NLSs) that target proteins to nuclei in oomycetes have not been defined, but assumed be the same as higher eukaryotes. Here, we use soybean pathogen Phytophthora sojae a model investigate these sequences oomycetes. By establishing reliable vivo NLS assay based on confocal microscopy, found many canonical monopartite and bipartite classical NLSs (cNLSs) mediated import poorly P. sojae. We efficient of by cNLSs requires additional basic amino acids at...
Gene regulation by small RNA pathways is ubiquitous among eukaryotes, but little known about in the Stramenopile kingdom. Phytophthora, a genus of filamentous oomycetes, contains many devastating plant pathogens, causing multibillion-dollar damage to crops, ornamental plants, and natural environments. The genomes several oomycetes including Phytophthora species such as soybean pathogen P. sojae, have been sequenced, allowing evolutionary analysis RNA-processing enzymes. This study examined...
Histone deacetylase 6 is a multifunctional lysine that recently emerging as central facilitator of response to stress and may play an important role in cancer cell proliferation. The histone 6-inhibitor tubacin has been shown slow the growth metastatic prostate cells sensitize chemotherapeutic agents. However, proteins interacts with, thus its cells, remains poorly characterized. activity be required for efficient basal autophagic flux. Autophagy often dysregulated confer resistance allow...
Abstract This study demonstrated that the ribosomal proteins Rpl22 and Rpl39 encoded by MAT locus of Cryptococcus neoformans are essential. Focusing on RPL22a RPL22α alleles, Ianiri et al. found differential expression two RPL22... The has a bipolar organization characterized an unusually large structure, spanning over 100 kb. genes have been functional genetics as being involved in sexual reproduction virulence. However, classical gene replacement failed to achieve mutants for five (RPL22,...
The phytochemical sulforaphane (SF) has been shown to decrease prostate cancer metastases in a genetic mouse model of carcinogenesis, though the mechanism action is not fully known. SF reported stimulate autophagy, and modulation autophagy proposed influence cytotoxicity; however, no conclusions about can be drawn without assessing autophagic flux, which characterized cells following treatment.We conducted an investigation assess impact on flux two metastatic cell lines at concentration...
Oomycetes are fungal-like eukaryotic microbes in the kingdom Stramenopila. We recently found that oomycete plant pathogen Phytophthora sojae uses nuclear localization signals (NLSs) for translocation of proteins into nucleus differ from conventional well-characterized NLSs mammals and yeast. Here, we have characterized depth a P. basic leucine zipper transcription factor, PsbZIP1. Nuclear PsbZIP1 was determined by central conserved region overlapping DNA binding domain. Mutational analysis...
SUMMARY Phytophthora sojae is a pathogenic oomycete that infects soybean seedlings as well stems and roots of established plants, costing growers $1–2 billion per year. Due to its economic importance, P. has become model for the study genetics, physiology pathology. Despite availability several genome sequences, lack efficient techniques targeted mutagenesis gene replacement have long hampered genetic studies pathogenicity in species. Here, we describe CRISPR/Cas9 system enabling rapid...
Abstract Phytophthora infestans , the causal agent of potato late blight, is a devastating plant disease that leads to Irish famine and threatens world-wide food security. Despite genome P. has provided fundamental resource for studying aggressiveness this pandemic pathogen, epigenomes remain poorly understood. Here, utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS), we demonstrate post-translational modifications (PTM) at core histone H3. The PTMs not only include these...
After the manuscript was accepted, inconsistencies in analyses were detected. These affected general conclusion of manuscript. This article retracted on 27 March 2024. A peer-reviewed revised version subsequently accepted: https://doi.org/10.1094/PHYTO-05-24-0172-R. Exserohilum turcicum is a devastating fungal pathogen that infects both maize and sorghum, leading to severe leaf diseases two crops. According host specificity, pathogenic isolates E. are divided into formae speciales, namely f....
Exserohilum turcicum is a devastating fungal pathogen that infects both maize and sorghum, leading to severe leaf diseases of the two crops. According host specificity, pathogenic isolates E. are divided into formae speciales, namely f. sp. zeae sorghi. To date, molecular mechanism underlying specificity marginally known. In this study, whole genomes 60 collected from sorghum were resequenced, which enabled identification 233,022 single-nucleotide polymorphisms (SNPs) in total. Phylogenetic...
Abstract Centromeres are chromosomal regions that serve as platforms for kinetochore assembly and spindle attachments, ensuring accurate chromosome segregation during cell division. Despite functional conservation, centromere DNA sequences diverse often repetitive, making them challenging to assemble identify. Here, we describe centromeres in an oomycete Phytophthora sojae by combining long-read sequencing-based genome chromatin immunoprecipitation the centromeric histone CENP-A followed...
Abstract Oomycetes are heterotrophic protists that share phenotypic similarities with fungi, including the ability to cause plant diseases, but branch in a separate and distant region of eukaryotic tree life. It has been suggested multiple horizontal gene transfers (HGTs) from fungi-to-oomycetes contributed evolution plant-pathogenic traits. These HGTs predicted include secreted proteins degrade cell walls. This is key trait pathology many oomycetes, as wall represents primary barrier...
Abstract Exserohilum turcicum is the causative agent of northern leaf blight maize and sorghum. Although host specificity E . has been observed from isolates collected sorghum, limited molecular evidence presented so far for formation formae speciales. In this study, 15 simple‐sequence repeat (SSR) markers were used to study genetic diversity population structure 60 sorghum in Henan province China. A total 54 haplotypes identified all isolates, which 29 unique 25 population. The phylogenetic...
COVID-19 disease become the most influential public health event in 2020. It has affected more than two hundred countries and regions. The prediction analysis of epidemic is extremely important. can help governments international organizations control development epidemic. In our study, we use three different models, namely, autoregressive integrated moving average (ARIMA), multilayer perceptron (MLP) long short-term memory (LSTM). We made predictions based on data from 20 countries, which...