A5015592178- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Yeasts and Rust Fungi Studies
- Mycorrhizal Fungi and Plant Interactions
- Plant pathogens and resistance mechanisms
- Fungal and yeast genetics research
- Plant Virus Research Studies
- Plant and fungal interactions
- Wheat and Barley Genetics and Pathology
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Plant and Fungal Interactions Research
- Lichen and fungal ecology
- Genetic diversity and population structure
- Fungal Biology and Applications
- Powdery Mildew Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Antifungal resistance and susceptibility
- Turfgrass Adaptation and Management
- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Parasitism and Resistance
- Genetic and phenotypic traits in livestock
North Carolina State University
2016-2025
North Central State College
2019-2025
Università Cattolica del Sacro Cuore
2018
Palacký University Olomouc
2017
Bar-Ilan University
2016
Hampton Roads Sanitation District
2011
Plant (United States)
2007
University of Toronto
1990-2001
Aurora College
1990-1999
A simple method is described for designing primer sets that can amplify specific protein-encoding sequences in a wide variety of filamentous ascomycetes. Using this technique, we successfully designed primers amplified the intergenic spacer region nuclear ribosomal DNA repeat, portions translation elongation factor 1 alpha, calmodulin, and chitin synthase genes, two other genes encoding actin ras protein. All amplicons were sequenced determined to target gene. Regions Sclerotinia...
A simple method is described for designing primer sets that can amplify specific protein-encoding sequences in a wide variety of filamentous ascomycetes. Using this technique, we successfully designed primers amplified the intergenic spacer region nuclear ribosomal DNA repeat, portions translation elongation factor 1 alpha, calmodulin, and chitin synthase genes, two other genes encoding actin ras protein. All amplicons were sequenced determined to target gene. Regions Sclerotinia...
Magnaporthe grisea is the most destructive pathogen of rice worldwide and principal model organism for elucidating molecular basis fungal disease plants. Here, we report draft sequence M. genome. Analysis gene set provides an insight into adaptations required by a fungus to cause disease. The genome encodes large diverse secreted proteins, including those defined unusual carbohydrate-binding domains. This also possesses expanded family G-protein-coupled receptors, several new...
Aspergillus flavus is the major producer of carcinogenic aflatoxins in crops worldwide and also an important opportunistic human pathogen aspergillosis. The sexual state this heterothallic fungus described from crosses between strains opposite mating type. Sexual reproduction occurred sexually compatible belonging to different vegetative compatibility groups. Multiple, indehiscent ascocarps containing asci ascospores formed within pseudoparenchymatous matrix stromata, which places genus...
Phytophthora infestans (Mont.) de Bary caused the 19th century Irish Potato Famine. We assessed genealogical history of P. using sequences from portions two nuclear genes (β- tubulin and Ras ) several mitochondrial loci P3, ( rpl 14, 5, tRNA) P4 Cox1 94 isolates South, Central, North America, as well Ireland. Summary statistics, migration analyses genealogy current populations for both are consistent with an “out South America” origin . Mexican putative center in Toluca Mexico harbored less...
To ensure future food security, it is crucial to understand how potential climate change scenarios will affect agriculture. One key area of interest climatic factors, both in the near- and long-term future, could fungal infection crops mycotoxin production by these fungi. The objective this paper review impact on three important mycotoxins that contaminate maize United States, highlight research questions approaches for understanding impact. Recent analyses pertain agriculture particular...
We have identified variants present in high-coverage complete sequences of 36 diverse human Y chromosomes from Africa, Europe, South Asia, East and the Americas, representing eight major haplogroups. After restricting our analysis to 8.97 Mb unique male-specific sequence, we 6662 high-confidence variants, including single-nucleotide polymorphisms (SNPs), multi-nucleotide (MNPs), indels. constructed phylogenetic trees using these or subsets them, recapitulated known structure tree. Assuming a...
Next-generation sequencing technologies have provided unprecedented insights into fungal diversity and ecology. However, intrinsic biases insufficient quality control in next-generation methods can lead to difficult-to-detect errors estimating community richness, distributions composition. The aim of this study was examine how tissue storage prior DNA extraction, primer design various quality-control approaches commonly used 454 amplicon pyrosequencing might influence ecological inferences...
Dollar spot is one of the most destructive and economically important fungal diseases amenity turfgrasses. The causal agent was first described in 1937 as ascomycete Sclerotinia homoeocarpa. However, genus-level taxonomic placement this fungus has been subject an ongoing debate for over 75 y. Existing morphological rDNA sequence evidence indicates that organism more appropriately placed family Rutstroemiaceae rather than Sclerotiniaceae. Here we use DNA data from samples dollar other members...
Abstract Understanding how species-rich communities persist is a foundational question in ecology. In tropical forests, tree diversity structured by edaphic factors, climate, and biotic interactions, with seasonality playing an essential role at landscape scales: wetter less seasonal forests typically harbor higher than more forests. We posited that the abiotic factors shaping extend to hyperdiverse symbionts leaves—fungal endophytes—that influence plant health, function, resilience stress....
Clonal lineages in the filamentous ascomycete (fungi) Sclerotinia sclerotiorum were determined by analysis of genealogies four loci: intergenic spacer nuclear ribosomal repeat (IGS; approximately 4 kb), translation elongation factor (EF-1α; 300 bp), an anonymous region (44.11; 700 and calmodulin gene (CAL; 400 bp). Three loci are physically unlinked. The combined provided best estimate phylogeny, which is consistent with a pattern some recombination among clonal against background...
Abstract Summary: The reconstruction of population processes from DNA sequence variation requires the coordinated implementation several coalescent-based methods, each bound by specific assumptions and limitations. In practice, application these methods for parameter estimation is difficult because they make strict that must be verified a priori their parameter-rich nature makes all model parameters very complex computationally intensive. A further complication distribution as console...
ABSTRACTBased on morphological and immunological studies, we hypothesize that there are two lineages within the Sclerotiniaceae, a family of plant-infecting ascomycetes in order Helotiales: 1) genera producing sclerotia, which tuberlike, melanized masses hyphae, 2) substratal stromata, mats compact hyphae incorporate plant tissues. We sequenced Internal Transcribed Spacer (ITS 1), defined by primers ITS 1 2, 43 isolates: 29 sclerotial isolates (19 species 9 genera), 11 (8 4 3 outgroup...
Abstract The genus Phytophthora includes some of the most destructive plant pathogens affecting agricultural and native ecosystems is responsible for a number recent emerging re‐emerging infectious diseases plants. Sudden oak death, caused by exotic pathogen P. ramorum , has extensive mortality oaks tanoaks in Northern California, brought economic losses to US European nurseries as well due its infection common ornamental In known range, occurs three distinct clonal lineages. We inferred...
Abstract Summary: We have added two software tools to our Suite of Nucleotide Analysis Programs (SNAP) for working with DNA sequences sampled from populations. SNAP Map collapses sequence data into unique haplotypes, extracts variable sites and manipulates output multiple formats input existing packages evolutionary analyses. includes novel features such as recoding insertions or deletions, including excluding that violate an infinite-sites model the option collapsing corresponding...
Little is known about the population structure of wheat powdery mildew in eastern United States, and most recent report on virulence this involved isolates collected 1993-94. In present study, leaves naturally infected with were from 10 locations southeastern States 2003 2005 a collection 207 was derived single ascospores. Frequencies to 16 resistance (Pm) genes determined by inoculating individually replicated plates detached differential lines. These frequencies used infer local...
Most of the species fungi that cause disease in mammals, including Cryptococcus neoformans var. grubii (serotype A), are exogenous and non-contagious. is associated worldwide with avian arboreal habitats. This airborne, opportunistic pathogen profoundly neurotropic leading fungal meningitis. Patients HIV/AIDS have been ravaged by cryptococcosis – an estimated one million new cases occur each year, mortality approaches 50%. Using phylogenetic population genetic analyses, we present evidence...
Abstract Aspergillus flavus is the major producer of carcinogenic aflatoxins (AFs) in crops worldwide. Natural populations A. show tremendous variation AF production, some which can be attributed to environmental conditions, differential regulation biosynthetic pathway and deletions or loss‐of‐function mutations gene cluster. Understanding evolutionary processes that generate genetic diversity may also explain quantitative differences aflatoxigenicity. Several population studies using...
Aflatoxins produced by Aspergillus flavus are potent carcinogens that contaminate agricultural crops. Recent efforts to reduce aflatoxin concentrations in crops have focused on biological control using nonaflatoxigenic A. strains AF36 (=NRRL 18543) and NRRL 21882 (the active component of afla-guard. However, the evolutionary potential these remain nature is unknown. To elucidate underlying population processes influence aflatoxigenicity, we examined patterns linkage disequilibrium (LD)...
Aspergillus flavus is the major producer of carcinogenic aflatoxins worldwide in crops. Populations A. are characterized by high genetic variation and source this likely sexual reproduction. The fungus heterothallic laboratory crosses produce ascospore-bearing ascocarps embedded within sclerotia. However, capacity for reproduction sclerotia naturally formed crops has not been examined. Corn was grown 3 years under different levels drought stress at Shellman, GA, were recovered from 146 ears...
High-quality phylogenetic placement of sequence data has the potential to greatly accelerate studies diversity, systematics, ecology and functional biology diverse groups. We developed Tree-Based Alignment Selector (T-BAS) toolkit allow evolutionary visualization DNA sequences representing unknown taxa within a robust context, permit downloading highly curated, single- multi-locus alignments for specific clades.In its initial form, T-BAS v1.0 uses core phylogeny 979 (including 23 outgroup...