- Plant Pathogens and Fungal Diseases
- Plant Pathogens and Resistance
- Yeasts and Rust Fungi Studies
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Mycotoxins in Agriculture and Food
- Mycorrhizal Fungi and Plant Interactions
- Powdery Mildew Fungal Diseases
- Nematode management and characterization studies
- Plant Disease Management Techniques
- Plant pathogens and resistance mechanisms
- Legume Nitrogen Fixing Symbiosis
- Banana Cultivation and Research
- Entomopathogenic Microorganisms in Pest Control
- Forest Insect Ecology and Management
- Postharvest Quality and Shelf Life Management
- Fungal Plant Pathogen Control
- Agronomic Practices and Intercropping Systems
- Turfgrass Adaptation and Management
- Wheat and Barley Genetics and Pathology
- Biological Control of Invasive Species
- Plant Parasitism and Resistance
- Plant Pathogenic Bacteria Studies
- Insect symbiosis and bacterial influences
- Plant Physiology and Cultivation Studies
Planta
2019-2021
Instituut voor Landbouw en Visserijonderzoek
2003-2019
Vlaams Instituut voor Biotechnologie
2019
Forest Research
2012-2013
Ghent University
2011
Horticultural Crops Research Laboratory
2010
United States Department of Agriculture
2010
Agricultural Research Service
2010
Julius Kühn-Institut
2010
Research Institute for Fisheries and Aquaculture
2009
The plant growth-promoting rhizobacterium Pseudomonas aeruginosa 7NSK2 produces three siderophores when iron is limited: the yellow-green fluorescent pyoverdin, salicylate derivative pyochelin, and salicylic acid. This strain was shown to be an efficient antagonist of Pythium-induced damping-off. role pyoverdin pyochelin in suppression Pythium splendens investigated by using various siderophore-deficient mutants derived from P. a bioassay with tomato (Lycopersicon esculentum). To provide...
The bacterium Xenorhabdus nematophila is a mutualist of the entomopathogenic nematode Steinernema carpocapsae. During its life cycle, exists both separately from and as an intestinal resident nonfeeding form, infective juvenile (IJ). progression X. ex vivo existence to specific persistent colonization IJs model understand mechanisms mediating initiation maintenance benign host-microbe interactions. To help characterize this process, we constructed strain that constitutively expresses green...
A multi-mycotoxin method was developed for the simultaneous determination of trichothecenes (nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, neosolaniol, fusarenon-X, diacetoxyscirpenol, HT-2 toxin, T-2 toxin), aflatoxins (aflatoxin-B(1), aflatoxin-B(2), aflatoxin-G(1) and aflatoxin-G(2)), Alternaria toxins (alternariol, alternariol methyl ether altenuene), fumonisins (fumonisin-B(1), fumonisin-B(2) fumonisin-B(3)), ochratoxin A, zearalenone, beauvericin...
Phytophthora ramorum, the cause of sudden oak death on and ramorum blight woody ornamentals, has been reported in ornamental nurseries West Coast North America from British Columbia to California. Long-distance migration P. occurred via nursery trade, shipments host plants are known have crossed U.S.-Canadian border. We investigated genotypic diversity Canadian compared population with U.S. European isolates for evidence among populations. All three clonal lineages were found Canada but,...
During a survey of Phytophthora diversity in natural ecosystems Taiwan six new species were detected. Multigene phylogeny based on the nuclear ITS, ß-tubulin and HSP90 mitochondrial cox1 NADH1 gene sequences demonstrated that they belong to ITS Clade 7a with P. europaea , uniformis rubi cambivora being their closest relatives. All differed from each other related by unique combination morphological characters, breeding system, cardinal temperatures growth rates. Four homothallic species,...
Calonectria pseudonaviculata , the causal agent of disease Buxus spp. known as ‘box blight’, was first detected in mid‐1990s UK and New Zealand. Since then, geographic range box blight has rapidly expanded to at least 21 countries throughout temperate regions world, causing significant losses nurseries, gardens wild boxwood populations. This study determined genetic diversity a collection 234 isolates from diseased plants, originating 15 four continents. Two clades, G1 G2, were identified...
ABSTRACT The production of an ant-deterrent factor(s) (ADF) by Xenorhabdus nematophila and Photorhabdus luminescens , the symbiotic bacteria nematodes Steinernema carpocapsae Heterorhabditis bacteriophora respectively, was examined. In addition to in vivo assay which were tested for their ability produce ADF within insect cadavers (M.E. Baur, H. K. Kaya, D. R. Strong, Biol. Control 12:231-236, 1998), vitro microtiter dish developed monitor activity produced grown cultures. Using these...
Summary One stage in the symbiotic interaction between bacterium Xenorhabdus nematophila and its nematode host, Steinernema carpocapsae , involves species‐specific colonization of intestinal vesicle by bacterium. To characterize bacterial molecular determinants that are essential for colonization, we adapted applied a signature‐tagged mutagenesis (STM) screen to this system. We identified 15 out 3000 transposon mutants X. with at least 15‐fold reduction average colonization. These harbour...
Real‐time PCR assays for Colletotrichum acutatum , one of the most important pathogens strawberry worldwide, were developed using primers designed to ribosomal DNA internal transcribed spacer 1 (rDNA ITS1) and β‐tubulin 2 gene. Using TaqMan technology, ITS‐based assay could reliably detect as little 50 fg genomic DNA, 100 copies target or 25 conidia. The β‐tubulin‐based was c . 66 times less sensitive, therefore suitable detection purposes. TaqMan‐ITS recognized all C. isolates tested from...
Phytophthora pseudosyringae is a self-fertile pathogen of woody plants, particularly associated with tree species from the genera Fagus, Notholithocarpus, Nothofagus and Quercus, which found across Europe in parts North America Chile. It can behave as soil infecting roots stem collar region, well an aerial leaves, twigs barks, causing particular damage United Kingdom western America. The population structure, migration potential outcrossing worldwide collection isolates were investigated...
Phytophthora is a long-established, well known and globally important genus of plant pathogens. Phylogenetic evidence has shown that the biologically distinct, obligate biotrophic downy mildews evolved from at least twice. Since, cladistically, this renders ‘paraphyletic’, it been proposed evolutionary clades be split into multiple genera (Runge et al. 2011; Crous 2021; Thines 2023; 2024). In letter, we review arguments for retention generic name with broad circumscription made by Brasier...
Co-existence of both mating types A1 and A2 within the EU1 lineage Phytophthora ramorum has only been observed in Belgium, which begs question whether sexual reproduction is occurring. A collection 411 Belgian P. isolates was established during a 7-year survey. Our main objectives were genetic characterization this population to test for reproduction, determination structure, evolution spread, evaluation effectiveness impact control measures. Novel, polymorphic simple sequence repeat (SSR)...
Abstract The genus Phytophthora comprises many economically and ecologically important plant pathogens. Hybrid species have previously been identified in at least six of the 12 phylogenetic clades. These hybrids can potentially infect a wider host range display enhanced vigour compared to their progenitors. therefore pose serious threat agriculture as well natural ecosystems. Early correct identification is essential for adequate protection but this hampered by limitations morphological...
ABSTRACT Burkholderia cepacia AMMDR1 is a biocontrol agent that protects pea and sweet corn seeds from Pythium damping-off in field experiments. The goal of this work was to understand the effect B. on aphanidermatum Aphanomyces euteiches zoospore homing events infection or roots. In vitro, caused lysis, prevented cyst germination, inhibited germ tube growth both oomycetes. also reduced attractiveness seed exudates zoospores nondetectable levels. However, when present at high levels seeds,...
Cylindrocladium buxicola (syn. C. pseudonaviculatum; teleomorph Calonectria pseudonaviculata) is an important fungal pathogen of Buxus spp. Although widespread in Western Europe, this has only recently been introduced into North America, where it represents a significant threat to the U.S. and Canadian boxwood industries. Trade latently infected nursery stock mode long-distance dissemination introduction but no methods for detection material are available. Also, pathways short-distance...
Wet sieving of soil samples, followed by plating on semi-selective medium and microscopic analysis, is the most commonly used technique to quantify microsclerotia-forming Verticillium species in soil. However, method restricted small does not allow easy differentiation between species, takes several weeks complete. This study describes an alternative test 100-g samples for three (V. tricorpus, V. dahliae, longisporum) using density flotation-based extraction microsclerotia new real-time...
Invasive oomycete pathogens have been causing significant damage to native ecosystems worldwide for over a century. A recent well‐known example is P hytophthora ramorum , the causal agent of sudden oak death, which emerged in 1990s E urope and N orth merica. In urope, this pathogen mainly restricted woody ornamentals nurseries public greens, while severe outbreaks wild only reported UK . This study presents results survey conducted Swiss between 2003 2011. all 120 subjected plant passport...
Trichoderma asperellum Samuels, Lieckf. & Nirenberg is one of several species comprising effective biocontrol strains. One these, T34, shows activity against soilborne pathogens such as Rhizoctonia, Fusarium, and Pythium. The strains T34 depends on their establishment in the rhizosphere. Monitoring population other rhizosphere growing medium therefore important for evaluating potential different crops systems. Until now, strain-specific monitoring has been very difficult main quantification...
Automatic detection of foliar diseases in potato fields, such as early blight caused by Alternaria solani, could allow farmers to reduce the application plant protection products while minimizing production losses. UAV-based, high resolution, NIR-sensitive cameras offer advantage a detailed top-down perspective, with high-contrast images ideally suited for detecting solani lesions. A field experiment was conducted 8 plots housing 256 infected plants which were monitored 6 times over 16-day...