A5102729517- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Yeasts and Rust Fungi Studies
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Plant Disease Management Techniques
- Forest Insect Ecology and Management
- Horticultural and Viticultural Research
- Plant Pathogenic Bacteria Studies
- Essential Oils and Antimicrobial Activity
- Plant and Fungal Interactions Research
- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Banana Cultivation and Research
- Fungal Plant Pathogen Control
- Mycotoxins in Agriculture and Food
- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Fungal Biology and Applications
- Fermentation and Sensory Analysis
- Powdery Mildew Fungal Diseases
- Plant pathogens and resistance mechanisms
- Nematode management and characterization studies
- Occupational health in dentistry
Universitat Politècnica de València
2014-2025
Mendel University in Brno
2021
Czech Academy of Sciences, Institute of Molecular Genetics
2021
Central University of Venezuela
2007-2017
Universitat de València
1999-2007
Instituto de Agroquímica y Tecnología de Alimentos
2007
Consejo Superior de Investigaciones Científicas
2007
Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar
1989-1999
Summary Oak decline has been a serious problem in Europe since the beginning of twentieth century. In south‐west Spain, Q uercus ilex and . suber are main affected species, their associated with P hytophthora cinnamomi During last 10 years, severe faginea accompanied by significant decrease production acorns affecting natural regeneration was observed eastern part Iberian Peninsula. Therefore, aim this study to investigate possible involvement Phytophthora spp. decline. A forest Natural Park...
During a survey of Phytophthora diversity in natural ecosystems Taiwan six new species were detected. Multigene phylogeny based on the nuclear ITS, ß-tubulin and HSP90 mitochondrial cox1 NADH1 gene sequences demonstrated that they belong to ITS Clade 7a with P. europaea , uniformis rubi cambivora being their closest relatives. All differed from each other related by unique combination morphological characters, breeding system, cardinal temperatures growth rates. Four homothallic species,...
In 2013 a survey of Phytophthora diversity was performed in 25 natural and seminatural forest stands rivers temperate montane subtropical lowland regions Taiwan. Using baiting assays, 10 described species 17 previously unknown taxa were isolated from 71.5% the 144 rhizosphere soil samples 33 40 tree sampled 24 stands, 19 rivers: P. capensis , citrophthora plurivora tropicalis citricola VII sp. × botryosa‐like, meadii‐like occultans‐like Clade 2; palmivora 4; castaneae heveae 5; chlamydospora...
During the winter of 2003-2004, dieback symptoms were observed on Pinus radiata and P. pinaster in pine nurseries Asturias (northern Spain). Small groups affected seedlings appeared randomly distributed throughout nurseries. The died rapidly, showing basal needle dieback, stem lesions, resin exudations, wilting. Isolations from infected material onto potato dextrose agar (PDA) supplemented with 0.5 mg/ml streptomycin sulfate Komada's medium consistently yielded Fusarium sp. cultures....
Fungal grapevine trunk diseases (GTDs) represent a threat to viticulture, being responsible for important economic losses worldwide. Nursery and vineyard experiments were set up evaluate the ability of Trichoderma atroviride SC1 reduce infections GTD pathogens in planting material during propagation process assess long-term protection provided by this biocontrol agent on plants young vineyards two growing seasons.Reductions some pathogen incidence severity found after nursery application T....
Diaporthe amygdali is the causal agent of twig canker and shoot blight disease on almond. The main objective this study was to elucidate effect weather variables D. inoculum in almond orchards Mediterranean conditions. For that purpose, a quantitative PCR (qPCR) assay for detection quantification developed. This methodology used detect quantify spore traps placed at two from different locations growing seasons (2019 2020 2021). Weather were also recorded. Two-part Hurdle models, which...
In this work, 82 Cylindrocarpon isolates associated with black foot disease of grapevines in Spain were studied by means phenotypical characterization, DNA analyses, and pathogenicity tests. Partial sequences the beta-tubulin (BT) gene, BT1, amplified using primers BT1a BT1b. A unique conserved 52-bp insertion BT1 sequence, which is a specific marker for C. macrodidymum, was found 56 isolates. The rest (26) identified as liriodendri. BT phylogeny grouped all each species into two...
Weeds were sampled in grapevine rootstock mother fields, open-root field nurseries, and commercial vineyards of Albacete, Alicante, Castellón, Murcia, Valencia provinces Spain between June 2009 2010 evaluated as potential hosts black-foot Petri disease pathogens. Isolations conducted the root system internal xylem tissues for pathogens, respectively. Cylindrocarpon macrodidymum was successfully isolated from roots 15 19 weed families 26 52 species. Regarding one isolate Phaeomoniella...
In this study, 31 almond orchards with trees showing severe decline symptoms were surveyed from 2009 to 2014 on the island of Mallorca (Spain). all, 45 Botryosphaeriaceae isolates collected and characterized based phenotypical features comparisons DNA sequence data nuclear ribosomal DNA-internal transcribed spacer region elongation factor 1-α gene. Five species identified as Diplodia olivarum, D. seriata, Neofusicoccum luteum, N. mediterraneum, parvum. Pathogenicity tests performed four...
Loquat ( Eriobotrya japonica ) is an important subtropical fruit crop in Spain and other Mediterranean countries. In recent years, characteristic symptoms of branch canker dieback have been observed the main cultivated areas loquat Spain. The goal this study was to identify characterize species Botryosphaeriaceae associated with these symptoms. For this, 36 affected orchards were surveyed between 2010 2011 six provinces southeastern Eighty‐four isolates belonging family recovered from...
The plant nursery industry has become an ideal reservoir for Phytophthora species and other soilborne pathogens. In this context, isolation from tissues soil of ornamental forest plants nurseries in four regions Spain was carried out. A high diversity confirmed. Fourteen phylotypes (P. cactorum, P. cambivora, cinnamomi, citrophthora, crassamura, gonapodyides, hedraiandra, nicotianae, niederhauserii, palmivora, plurivora, pseudocryptogea, sansomeana, sp. tropicalis-like 2) were isolated over...
Summary. During the last five years a decline of table grape plants has been noticed in nurseries, young planta- tions and vineyards Northeastern region Brazil, where management systems for grapevine production are adapted to specific environmental conditions tropical viticulture. Samples show- ing symptoms were obtained from plantations located Sao Francisco, Assu Siriji Valleys 2010, subjected fungal isolation. Grapevine trunk pathogens identified using morphological molecular methods....
Juglans regia L. (English walnut) trees with cankers and dieback symptoms were observed in two regions the Czech Republic. Isolations made from diseased branches. In total, 138 fungal isolates representing 10 species obtained wood samples identified based on morphological characteristics molecular methods: Cadophora novi-eboraci, spadicis, Cryptovalsa ampelina, Diaporthe eres, Diplodia seriata, Dothiorella omnivora, Eutypa lata, Eutypella sp., Peroneutypa scoparia, Phaeoacremonium...
Two hundred and twenty-five Diaporthe isolates were collected from 2005 to 2019 in almond orchards showing twig cankers shoot blight symptoms five different regions across Spain. Multilocus DNA sequence analysis with loci (ITS, tub, tef-1α, cal his), allowed the identification of four known species, namely: D. amygdali, eres, foeniculina phaseolorum. Moreover, a novel phylogenetic mediterranea, was described. amygdali most prevalent due largest number (85.3%) obtained all sampled regions....
Cadophora luteo-olivacea is a lesser-known fungal trunk pathogen of grapevine which has been recently isolated from vines showing decline symptoms in grape growing regions worldwide. In this study, 80 C. isolates (65 Spain and 15 South Africa) were studied. Inter-simple-sequence repeat-polymerase chain reaction (ISSR-PCR) generated 55 polymorphic loci four ISSR primers selected an initial screen 13 primers. The markers revealed 40 multilocus genotypes (MLGs) the global population. Minimum...
Abstract In this work, a collection of 94 Macrophomina isolates obtained from roots two weed species, Trianthema portulacastrum and Boerhavia diffusa, collected during surveys conducted 2015 2016 in melon production fields Northeastern Brazil, were characterized by using molecular techniques. Phylogenetic analysis the EF1‐α gene allowed identification 32 as M. phaseolina 62 pseudophaseolina . Results pathogenicity test performed on seedlings cv. “Gladial” revealed that all inoculated able to...
Four vineyards visibly affected by trunk diseases were surveyed at pruning time in 2012 and 2013 Spain, to determine whether tools are capable of spreading grapevine from vine vine. In each vineyard, shears regularly rinsed with sterile water, collecting liquid samples for analysis. Molecular detection fungal pathogens (GFTPs) was performed nested polymerase chain reaction using specific primers detect Botryosphaeriaceae spp. Eutypa lata, Cadophora luteo-olivacea, Phaeoacremonium spp.,...