A5059191462- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Environmental DNA in Biodiversity Studies
- Microbial Community Ecology and Physiology
- Plant-Microbe Interactions and Immunity
- Plant and Fungal Interactions Research
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Insect symbiosis and bacterial influences
- Studies on Chitinases and Chitosanases
- Identification and Quantification in Food
- Microplastics and Plastic Pollution
- Genetic and phenotypic traits in livestock
- Wheat and Barley Genetics and Pathology
- Plant tissue culture and regeneration
- Recycling and Waste Management Techniques
- Genetic Mapping and Diversity in Plants and Animals
- Gut microbiota and health
- Turfgrass Adaptation and Management
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Resistance
- Plant Parasitism and Resistance
- Plant and animal studies
Instituut voor Landbouw en Visserijonderzoek
2020-2024
Vlaams Instituut voor Biotechnologie
2006-2024
Planta
2019-2021
Ghent University Hospital
2013-2021
Ghent University
2006-2018
University of Liège
2018
Gembloux Agro-Bio Tech
2018
Institute of Plant Genetics, Polish Academy of Sciences
2006
Bacterial colonization of marine plastic litter (MPL) is known for over four decades. Still, only a few studies on the process and its influencing factors are reported. In this study, seafloor MPL was sampled at different locations across Belgian part North Sea to study bacterial community structure using 16S metabarcoding. These communities were compared with those sediment seawater, resin pellets beach, investigate origin uniqueness communities. Plastics display great variation...
Despite growing evidence that biofilm formation on plastic debris in the marine environment may be essential for its biodegradation, underlying processes have yet to fully understood. Thus, far, bacterial had only been studied after short-term exposure or floating plastic, a prominent share of litter accumulates seafloor. In this study, we explored taxonomic composition and fungal communities polyethylene sheets dolly ropes during long-term seafloor, both at harbor an offshore location...
Summary Rice is one of the most important staple crops worldwide, but its yield compromised by different pathogens, including plant‐parasitic nematodes. In this study we have characterized specific and general responses rice ( O ryza sativa ) roots challenged with two endoparasitic nematodes very modes action. Local transcriptional changes in upon root knot M eloidogyne graminicola rot nematode (RRN, H irschmanniella oryzae infection were studied at time points (3 7 d after infection, dai),...
One of the reasons for progressive yield decline observed in aerobic rice production is rapid build-up populations root knot nematode Meloidogyne graminicola. These nematodes induce specialized feeding cells inside tissue, called giant cells. By injecting effectors and sipping metabolites out these cells, they reprogramme normal cell development deprive plant its nutrients. In this research we have studied transcriptome rice, after isolation by laser-capture microdissection. The expression...
Recent developments in high-throughput sequencing (HTS), also called next-generation (NGS), technologies and bioinformatics have drastically changed research on viral pathogens spurred growing interest the field of virus diagnostics. However, reliability HTS-based detection protocols must be evaluated before adopting them for Many different algorithms aimed at detecting viruses HTS data been reported but little attention has paid thus far to their sensitivity diagnostic purposes. Therefore,...
Abstract Background When maize ( Zea mays L.) is grown in the Northern hemisphere, its development heavily arrested by chilling temperatures, especially at juvenile phase. As some endophytes are beneficial for plants under stress conditions, we analyzed impact of temperatures on root microbiome and examined whether microbiome-based analysis might help to identify bacterial strains that could promote growth these temperatures. Results We investigated how composition changed means 16S rRNA...
Abstract Background Microorganisms are not only indispensable to ecosystem functioning, they also keystones for emerging technologies. In the last 15 years, number of studies on environmental microbial communities has increased exponentially due advances in sequencing technologies, but large amount data generated remains difficult analyze and interpret. Recently, metabarcoding analysis shifted from clustering reads using Operational Taxonomical Units (OTUs) Amplicon Sequence Variants (ASVs)....
Chitin is a promising soil amendment for improving quality, plant growth, and resilience. The objectives of this study were twofold. First, to the effect chitin mixed in potting on lettuce growth survival two zoonotic bacterial pathogens, Escherichia coli O157:H7 Salmonella enterica leaves. Second, assess related changes microbial rhizosphere, using phospholipid fatty acid (PLFA) analysis amplicon sequencing 16S rRNA gene fragment fungal ITS2. As result addition, fresh yield weight was...
High-throughput sequencing (HTS) technologies have the potential to become one of most significant advances in molecular diagnostics. Their use by researchers detect and characterize plant pathogens pests has been growing steadily for more than a decade they are now envisioned as routine diagnostic test be deployed pest diagnostics laboratories. Nevertheless, HTS downstream bioinformatics analysis generated datasets represent complex process including many steps whose reliability must...
The rumen microbiome occupies a central role in animal health and productivity. A better understanding of the ecosystem is essential to increase productivity or decrease methane production. Samples were collected from three main environments: solid-adherent fraction, liquid fraction epithelium. For solid two alternative sample processing protocols compared, resulting total five types: crude solids (S), eluted (Ad), free-living species (CRL), strained samples (Lq) epimural scrapings (Ep)....
Adding biochar, the solid coproduct of biofuel production, to peat can enhance strawberry growth, and disease resistance against airborne fungal pathogen Botrytis cinerea. Additionally, biochar induce shifts in rhizosphere microbiome. However, moment that this biochar-mediated shift occurs is not known. Further, effect an above-ground infection on microbiome unknown. In present study we established two experiments which transplants (cv. Elsanta) were planted either or amended with 3%...
Plant-parasitic root-knot nematodes induce the formation of giant cells within plant root, and it has been recognized that auxin accumulates in these feeding sites. Here, we studied role transport system governed by AUX1/LAX3 influx proteins different PIN efflux during site development Arabidopsis thaliana roots. Data generated via promoter-reporter line protein localization analyses evoke a model which is being imported at basipetal side concerted action AUX1 LAX3, PIN3. Mutants LAX3 bear...
Nematodes are ideal biological indicators to monitor soil biodiversity and ecosystem functioning. For this reason, they have been receiving increasing attention from a broad range of scientists. The main method characterize nematode communities until at least genus level is still based on microscopic observations morphology. Such an approach time-consuming, labor-intensive, requires specialized personnel. first studies the potential use DNA-metabarcoding showed some shortcomings: under- or...
Abstract To effectively monitor, manage and protect aquatic species understand their interactions, knowledge of spatiotemporal distribution is needed. In this study, we used a fine‐scale water sampling design, followed by environmental DNA (eDNA) 12S metabarcoding, to investigate occupancy patterns natural community fish amphibian in lentic system. the same system, experimentally estimated spatial temporal dispersion eDNA placing different cages at one side pond, creating controlled point...
On invasion of roots, plant-parasitic nematodes secrete effectors to manipulate the cellular regulation host promote parasitism. The root-knot nematode Meloidogyne graminicola is one most damaging rice. Here, we identified a novel effector this nematode, named Mg16820, expressed in subventral glands. We localized Mg16820 apoplast during migration phase second-stage juvenile rice roots. In addition, early development feeding site, was giant cells, where it accumulated cytoplasm and nucleus....
Abstract Chitin is a valuable peat substrate amendment by increasing lettuce growth and reducing the survival of zoonotic pathogen Salmonella enterica on leaves. The production chitin-catabolic enzymes (chitinases) play crucial role are mediated through microbial community. A higher abundance plant-growth promoting microorganisms genera involved in N chitin metabolism present chitin-enriched substrate. In this study, we hypothesize that addition to stimulates chitinase production....
Abstract High‐throughput sequencing (HTS) is a powerful tool that enables the simultaneous detection and potential identification of any organisms present in sample. The growing interest application HTS technologies for routine diagnostics plant health laboratories triggering development guidelines on how to prepare performing testing. This paper describes general technical recommendations guide through complex process preparing laboratory tests within existing quality assurance systems....
Horizon scans have emerged as a valuable tool to anticipate the incoming invasive alien species (IAS) by judging on their potential impacts. However, little research has been conducted quantifying actual impacts and assessing causes of species-specific vulnerabilities particular IAS due persistent methodological challenges. The underlying interspecific mechanisms driving therefore remain poorly understood, even though they can substantially improve accuracy risk assessments. Given that...