A5026453326- Helicobacter pylori-related gastroenterology studies
- Inflammatory mediators and NSAID effects
- Gastroesophageal reflux and treatments
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Protein Structure and Dynamics
- RNA modifications and cancer
- Advanced NMR Techniques and Applications
- Eosinophilic Esophagitis
- Potassium and Related Disorders
- Drug Transport and Resistance Mechanisms
- Neuropeptides and Animal Physiology
- Gastrointestinal motility and disorders
- Enzyme Structure and Function
- Gastric Cancer Management and Outcomes
- Listeria monocytogenes in Food Safety
- Molecular spectroscopy and chirality
- Ion Transport and Channel Regulation
- Diabetes Treatment and Management
- Eicosanoids and Hypertension Pharmacology
- Adipose Tissue and Metabolism
- RNA regulation and disease
- Receptor Mechanisms and Signaling
- NMR spectroscopy and applications
- Mast cells and histamine
European Molecular Biology Laboratory
2016-2025
European Molecular Biology Laboratory
2008-2025
UConn Health
2023-2024
Heidelberg University
1980-2023
Weizmann Institute of Science
2023
DKFZ-ZMBH Alliance
2023
University of Geneva
2023
Institute of Structural and Molecular Biology
2020
European Molecular Biology Organization
2003-2013
European Bioinformatics Institute
2005-2011
This article develops a social psychological model of politicized collective identity that revolves around 3 conceptual triads. The 1st triad consists identity, the struggle between groups for power, and wider societal context. It is proposed people evince to extent they engage as self-conscious group members in power on behalf their knowing it more inclusive context which this has be fought out. Next, antecedent stages leading are distinguished: awareness shared grievances, adversarial...
Background— Arrhythmogenic right ventricular cardiomyopathy (ARVC) is an inherited genetic myocardial disease characterized by fibrofatty replacement of the myocardium and a predisposition to cardiac arrhythmias sudden death. We evaluated gene titin ( TTN ) as candidate ARVC because its proximity locus at position 2q32 connection protein transitional junction intercalated disks. Methods Results— All 312 exons known be expressed in human complete 3′ untranslated region were sequenced 38...
Abstract Bromodomains are critical components of many chromatin modifying/remodelling proteins and emerging therapeutic targets, yet how they interact with nucleosomes, rather than acetylated peptides, remains unclear. Using BRDT as a model, we characterized the BET family bromodomains interacts site-specifically nucleosomes. Here report that nucleosomes through its first (BD1), but not second (BD2) bromodomain, histone recognition by BD1 is complemented bromodomain–DNA interaction....
Abstract Post-transcriptional mechanisms play a predominant role in the control of microRNA (miRNA) production. Recognition terminal loop precursor miRNAs by RNA-binding proteins (RBPs) influences their processing; however, mechanistic basis for how levels individual or subsets are regulated is mostly unexplored. We previously showed that hnRNP A1, an RBP implicated many aspects RNA processing, acts as auxiliary factor promotes Microprocessor-mediated processing pri-mir-18a. Here, using...
The condensin protein complex plays a key role in the structural organization of genomes. How ATPase activity its SMC subunits drives large-scale changes chromosome topology has remained unknown. Here we reconstruct, at near-atomic resolution, sequence events that take place during cycle. We show ATP binding induces conformational switch Smc4 head domain releases hitherto undescribed interaction with Ycs4 HEAT-repeat subunit and promotes engagement Smc2 into an asymmetric heterodimer....
Abstract Dynein and kinesin motors mediate long-range intracellular transport, translocating towards microtubule minus plus ends, respectively. Cargoes often undergo bidirectional transport by binding to both simultaneously. However, it is not known how motor activities are coordinated in such circumstances. In the Drosophila female germline, sequential of dynein–dynactin–BicD–Egalitarian (DDBE) complex kinesin-1 deliver oskar messenger RNA from nurse cells oocyte, within oocyte posterior...
Putting the pieces together: An efficient, generally applicable approach for structural analysis of protein complexes and multidomain proteins in solution based on NMR spectroscopy is presented. Starting from available high-resolution structures individual domains or subunits, overall domain arrangement calculated data that can be obtained high-molecular-weight complexes. Detailed facts importance to specialist readers are published as ”Supporting Information”. Such documents peer-reviewed,...
Abstract Knowledge of the RNA three-dimensional structure, either in isolation or as part RNP complexes, is fundamental to understand mechanism numerous cellular processes. Because its flexibility, represents a challenge for crystallization, while large size complexes brings solution-state NMR limits. Here, we demonstrate an alternative approach on basis solid-state spectroscopy. We develop suite experiments and labeling schemes first time that ssNMR can yield structure at high-resolution....
Striated muscle undergoes remodelling in response to mechanical and physiological stress, but little is known about the integration of such varied signals myofibril.The interaction elastic kinase region from sarcomeric titin (A168-M1) with autophagy receptors Nbr1/p62 MuRF E3 ubiquitin ligases well suited link mechanosensing trophic myofibril.To investigate mechanisms signal cross-talk at this node, we elucidated its 3D structure, analysed stretch using steered molecular dynamics simulations...
During mRNA elongation, the SRI domain of histone H3 methyltransferase Set2 binds to phosphorylated carboxyl-terminal (CTD) RNA polymerase II. The solution structure yeast reveals a novel CTD-binding fold consisting left-handed three-helix bundle. NMR titration shows that an Ser2/Ser5-phosphorylated CTD peptide comprising two heptapeptide repeats and three flanking NH2-terminal residues, whereas single repeat is insufficient for binding. Residues show strong chemical shift perturbations upon...
Article5 February 2009free access Structural basis for competitive interactions of Pex14 with the import receptors Pex5 and Pex19 Christian Neufeld EMBL Heidelberg, Germany Hamburg Outstation, c/o DESY, Hamburg, Search more papers by this author Fabian V Filipp GermanyPresent address: Department Chemistry Biochemistry, University California, San Diego, La Jolla, CA 92093-0307, USA Bernd Simon Alexander Neuhaus Institute Physiological Chemistry, Systems Biology, Faculty Medicine, Ruhr Bochum,...