A5035157190- Cervical Cancer and HPV Research
- Hepatitis B Virus Studies
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Toxin Mechanisms and Immunotoxins
- RNA and protein synthesis mechanisms
- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
- Genetics and Neurodevelopmental Disorders
- Animal Virus Infections Studies
- Genital Health and Disease
- Metabolism and Genetic Disorders
- Cancer-related Molecular Pathways
- Polyomavirus and related diseases
- Monoclonal and Polyclonal Antibodies Research
- PARP inhibition in cancer therapy
- Renal function and acid-base balance
- RNA modifications and cancer
- Clostridium difficile and Clostridium perfringens research
- Nonmelanoma Skin Cancer Studies
- Poxvirus research and outbreaks
- interferon and immune responses
- vaccines and immunoinformatics approaches
Heidelberg University
2009-2024
European Molecular Biology Laboratory
2014-2024
European Molecular Biology Organization
2012-2021
German Cancer Research Center
2001-2020
European Molecular Biology Laboratory
2010-2016
European Bioinformatics Institute
2007-2014
University of Konstanz
2007
DKFZ-ZMBH Alliance
2001-2006
University Hospital Heidelberg
2006
Istituto Nazionale Tumori IRCCS "Fondazione G. Pascale"
2006
More than 100 different human papillomaviruses (HPVs) can cause proliferative diseases, many of which are malignant, such as cervical cancer. HPV serology is complex because infection and disease lead to distinct type-specific antibody responses. Using bead-based technology, we have developed an assay platform that allows the simultaneous detection antibodies against up in situ affinity-purified recombinant proteins.Twenty-seven proteins were expressed glutathione S-transferase fusion one...
An increase in worldwide HPV vaccination could be facilitated if fewer than three doses of vaccine are as effective doses. We originally aimed to compare the immunogenicity and frequency persistent infection cervical precancerous lesions caused by vaccine-targeted after with two quadrivalent on days 1 180 or later, 1, 60, a cluster-randomised trial. Suspension recruitment due events unrelated our study meant that some enrolled girls not vaccinated received planned number vaccinations...
Background: Although infection with human papillomaviruses (HPVs) is a major risk factor for several epithelial cancers, an etiologic relationship between HPV and keratinocyte such as squamous cell carcinomas (SCCs) basal (BCCs), remains unclear. Methods: In population-based case–control study of 252 SCC case patients, 525 BCC 461 control subjects, we used multiplex serology to detect antibodies in plasma samples against 16 types from phylogenetic genera alpha, beta, mu. Multiplex new method...
The hydrogen ion activity within isolated chromaffin granules can be estimated from the distribution of weak base methylamine and phosphorus-31 nuclear magnetic resonance spectra ATP contained in granules. Following addition to external medium, internal pH drops by 0.2 0.5 unit. This change occurs only medium containing a permeant anion such as chloride is abolished an uncoupler oxidative phosphorylation. These results indicate that granule membrane possess electrogenic proton pump directed inward.
We studied the effects of glucosylation RhoA, Rac1, and Cdc42 at threonine-35 -37 by Clostridium difficile toxin B on nucleotide binding, GTPase activity, effector coupling compared these results with ADP ribosylation RhoA asparagine-41 catalyzed botulinum C3 transferase. Whereas had no major GDP release from Cdc42, rate GTPgammaS Rho proteins was increased 3-6-fold glucosylation. decreased about 50%. Glucosylation reduced intrinsic activities GTPases 3-7-fold completely blocked stimulation...
The natural history of infections with many human papillomavirus (HPV) types is poorly understood. Here, we describe for the first time age- and sex-dependent antibody prevalence 29 cutaneous five mucosal HPV from 15 species within phylogenetic genera (alpha, beta, gamma, mu, nu) in a general population. Sera 1,797 German adults children (758 males 1,039 females) between 1 82 years (median 37 years) were analysed antibodies to major capsid protein L1 by Luminex-based multiplex serology....
Human papillomavirus (HPV) vaccination is a major strategy for preventing cervical and other ano-genital cancers. Worldwide HPV introduction coverage will be facilitated if single dose of vaccine as effective two or three doses demonstrates significant protective effect compared to ‘no vaccination’. In multi-centre cluster randomized trial vs quadrivalent (Gardasil™) in India, suspension the due events unrelated study led per protocol partial unmarried 10–18 year old girls leading four...
The discovery of isozyme-selective histone deacetylase (HDAC) inhibitors is critical for understanding the biological functions individual HDACs and validating as drug targets. isozyme HDAC10 contributes to chemotherapy resistance has recently been described be a polyamine deacetylase, but no studies toward selective have published. Using two complementary assays, we found Tubastatin A, an HDAC6 inhibitor, potently bind HDAC10. We synthesized A derivatives that basic amine in cap group was...
The recent World Health Organization recommendation supporting single-dose of HPV vaccine will significantly reduce programmatic cost, mitigate the supply shortage, and simplify logistics, thus allowing more low- middle-income countries to introduce vaccine. From a perspective durability protection offered by be key consideration. primary objectives present study were determine whether recipients quadrivalent had sustained immune response against targeted types (HPV 6,11,16,18) at 10 years...
The purpose is to study the immunogenicity of heterologous prime-boost human papillomavirus (HPV) oncogene vaccination in patients with anogenital intraepithelial neoplasia (AGIN).Twenty-nine women high-grade AGIN received three i.m. doses TA-CIN (HPV-16 L2/E6/E7 protein) at four weekly intervals followed by a single dermal scarification vaccinia HPV-16/18 E6/E7 and were up for 12 weeks. Immunity HPV-16 was assessed lymphoproliferation, IFN-gamma enzyme-linked immunospot (ELISPOT), ELISA.The...
Chromaffin granules suspended in C1-containing media release catecholamine and protein when ATP is added. This phenomenon inhibited hyperosmotic the presence of uncouplers oxidative phosphorylation. Release requires a permeant anion medium, but independent cation. The process appears to be driven by an inwardly directed proton-translocating adenosine triphosphatase. resulting proton-anion influx causes osmotic lysis chromaffin granules.
Abstract Background Muscle ring finger 1 (MuRF1) is a muscle‐specific ubiquitin E3 ligase activated during clinical conditions associated with skeletal muscle wasting. Yet, there remains paucity of therapeutic interventions that directly inhibit MuRF1 function, particularly in vivo . The current study, therefore, developed novel compound targeting the central coiled coil domain to wasting cardiac cachexia. Methods We identified small molecules interfere MuRF1–titin interaction from 130 000...
The human papillomavirus (HPV) minor capsid protein L2 is a promising candidate for broadly protective HPV vaccine yet the titers obtained in most experimental systems are rather low. Here we examine potential of empty AAV2 particles (AAVLPs), assembled from VP3 alone, display epitopes to enhance their immunogenicity. Insertion neutralizing epitope (amino acids 17–36) HPV16 and HPV31 into at positions 587 453, respectively, permitted assembly AAV (AAVLP(HPV16/31L2)). Intramuscularly...
Background. We compared the immunogenicity and reactogenicity of Cervarix or Gardasil human papillomavirus (HPV) vaccines in adults infected with immunodeficiency virus (HIV). Methods. This was a double-blind, controlled trial randomizing HIV-positive to receive 3 doses at 0, 1.5, 6 months. Immunogenicity evaluated for up 12 Neutralizing anti–HPV-16/18 antibodies were measured by pseudovirion-based neutralization assay. Laboratory tests diary cards used safety assessment. The HPV-DNA status...
The heterotrimeric influenza virus polymerase performs replication and transcription of viral RNA in the nucleus infected cells. Transcription by "cap-snatching" requires that host-cell pre-mRNAs are bound via their 5' cap to PB2 subunit. Thus, cap-binding site is potentially a good target for new antiviral drugs will directly inhibit replication. Docking studies using structure domain suggested 7-alkylguanine derivatives substituted at position N-9 N-2 could be candidates. Four series...