A5024082166- Influenza Virus Research Studies
- RNA and protein synthesis mechanisms
- Respiratory viral infections research
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- interferon and immune responses
- RNA modifications and cancer
- Animal Disease Management and Epidemiology
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Plant Virus Research Studies
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Bacterial Genetics and Biotechnology
- Advanced biosensing and bioanalysis techniques
- Respiratory Support and Mechanisms
- Vector-Borne Animal Diseases
- Molecular Biology Techniques and Applications
Consejo Superior de Investigaciones Científicas
1995-2020
Centro Nacional de Biotecnología
2009-2020
Instituto de Salud Carlos III
1994-2017
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
2008-2017
Centro de Investigación Biomédica en Red
2009-2016
Fundación Juan March
2009
Unidades Centrales Científico-Técnicas
2007
Ministry of Health
2006
National Center for Infectious Diseases
2006
Centers for Disease Control and Prevention
2006
Avian influenza A H5N1 viruses continue to spread globally among birds, resulting in occasional transmission of virus from infected poultry humans. Probable human-to-human has been documented rarely, but have not yet acquired the ability transmit efficiently humans, an essential property a pandemic virus. The pandemics 1957 and 1968 were caused by avian-human reassortant that had human virus-like receptor binding properties. However, relative contribution internal protein genes or other...
The influenza viruses cause annual epidemics of respiratory disease and occasional pandemics, which constitute a major public-health issue. segmented negative-stranded RNAs are associated with the polymerase complex nucleoprotein (NP), forming ribonucleoproteins (RNPs), responsible for virus transcription replication. We describe structure native RNPs derived from virions. They show double-helical conformation in two NP strands opposite polarity each other along helix. Both connected by...
In hippocampal neurons, certain mRNAs have been found in dendrites (), and their localization translation implicated synaptic plasticity (). One attractive candidate to achieve transport of into is Staufen (Stau), a double-stranded RNA-binding protein, which plays pivotal role mRNA transport, localization, Drosophila Using antibodies raised against peptide located the domain IIa polyclonal antibody recently cloned human homolog, we identify 65 kDa rat homolog cultured neurons. agreement with...
Influenza virus NS1 protein is an RNA-binding whose expression alters several posttranscriptional regulatory processes, like polyadenylation, splicing, and nucleocytoplasmic transport of cellular mRNAs. In addition, enhances the translational rate viral, but not cellular, To characterize this effect, we looked for targets influenza among translation factors. We found that coimmunoprecipitates with eukaryotic initiation factor 4GI (eIF4GI), large subunit cap-binding complex eIF4F, either in...
The influenza viruses contain a segmented, single-stranded RNA genome of negative polarity. Each segment is encapsidated by the nucleoprotein and polymerase complex into ribonucleoprotein particles (RNPs), which are responsible for virus transcription replication. Despite their importance, information about structure these RNPs scarce. We have determined three-dimensional biologically active recombinant RNP cryo-electron microscopy. shows nonameric ring (at 12 Angstrom resolution) with two...
The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in mammalian cells-Escherichia coli shutle vector. When this construction was introduced into VERO cells it expressed relevant enzymic activity. Moreover, used as dominant marker for selection of transformed able grow presence antibiotic.
The influenza A viruses genome comprises eight single-stranded RNA segments of negative polarity. Each one is included in a ribonucleoprotein particle (vRNP) containing the polymerase complex and number nucleoprotein (NP) monomers. Viral replication proceeds by formation complementary RNP positive polarity (cRNP) that serves as intermediate to generate many progeny vRNPs. Transcription initiation takes place cap-snatching mechanism whereby steals cellular capped oligonucleotide uses it...
It has previously been shown that influenza virus NS1 protein enhances the translation of viral but not cellular mRNAs. This enhancement occurs by increasing rate initiation and requires 5'UTR sequence, common to all In agreement with these findings, we show here mRNAs, are associated during infection. We have reported interacts factor eIF4GI, next its poly(A)-binding 1 (PABP1)-interacting domain eIF4GI in virus-infected cells. Here NS1, although capable binding poly(A), does compete PABP1...
The effect of NS1 protein on the efficiency influenza virus mRNA translation was evaluated by determining accumulation nucleoprotein (NP) or M1 mRNAs in cytoplasm cells expressing either these genes alone combination with gene, as well total cell NP protein. Coexpression NS1, but not NS2 protein, led to increases range 5- 100-fold. This enhancement specific for viral mRNAs, since neither cat nor lacZ affected coexpression use chimeric containing 5'-extracistronic sequences corresponding...
In the course of a two-hybrid screen with NS1 protein influenza virus, human clone capable coding for high homology to Staufen from Drosophila melanogaster (dmStaufen) was identified. With these sequences used as probe, cDNAs were isolated λ cDNA library. The encoded (hStaufen-like) contained four double-stranded RNA (dsRNA)-binding domains 55% similarity and 38% identity those dmStaufen, including at all residues involved in binding. A recombinant containing dsRNA-binding expressed...
The gene product of open reading frame 5 (p25) porcine reproductive and respiratory syndrome (PRRS) virus has been expressed by coinfection culture cells with vaccinia expressing the T7 RNA polymerase a recombinant encoding under promoter encephalomyocarditis internal ribosome entry site. In spite reported efficiency expression system, very poor accumulation p25 protein was observed strong cytotoxicity produced in doubly infected cells. This cell toxicity shown to occur induction apoptosis,...
The domains of the PB1 subunit influenza virus polymerase involved in interaction with PB2 and PA subunits have been defined by mutational analysis protein. experimental approach included vivo competition activity, two-hybrid assays vitro binding to PBI-specific matrices. Mutants gene including N-terminal, C-terminal internal deletions single amino acid insertions were constructed. They unable support activity a reconstituted transcription-replication system tested for their when expressed...
Abstract The influenza virus polymerase is formed by the PB1, PB2 and PA subunits required for transcription replication in nucleus of infected cells. Here we present characterisation complexes intracellularly human was expressed cotransfection cDNAs, one which fused to tandem‐affinity purification (TAP) tag. intracellular were purified TAP approach, involves IgG‐Sepharose calmodulin‐agarose chromatography, under very mild conditions. contained heterotrimeric a series associated proteins...
The adenovirus type 12-specific mRNA and the stable nuclear RNA from productively infected KB cells, early postinfection, abortively BHK-21 12-transformed hamster lines T637 HA12/7 have been mapped on genome of 12. intact separated heavy (H) light (L) strands 12 DNA used to determine extent complementarity or different cell each strands. More precise map positions obtained by use H L complements fragments which were produced with EcoRI BamHI restriction endonucleases. results mapping...